Comparative genomics analysis of a new Exiguobacterium strain from salar de huasco reveals a repertoire of stress-related genes and arsenic resistance

Juan Castro-Severyn, Francisco Remonsellez, Sandro L. Valenzuela, Cesar Salinas, Jonathan Fortt, Pablo Aguilar, Coral Pardo-Esté, Cristina Dorador, Raquel Quatrini, Franck Molina, Daniel Aguayo, Eduardo Castro-Nallar, Claudia P. Saavedra

Resultado de la investigación: Article

5 Citas (Scopus)

Resumen

The Atacama Desert hosts diverse ecosystems including salt flats and shallow Andean lakes. Several heavy metals are found in the Atacama Desert, and microorganisms growing in this environment show varying levels of resistance/tolerance to copper, tellurium, and arsenic, among others. Herein, we report the genome sequence and comparative genomic analysis of a new Exiguobacterium strain, sp. SH31, isolated from an altiplanic shallow athalassohaline lake. Exiguobacterium sp. SH31 belongs to the phylogenetic Group II and its closest relative is Exiguobacterium sp. S17, isolated from the Argentinian Altiplano (95% average nucleotide identity). Strain SH31 encodes a wide repertoire of proteins required for cadmium, copper, mercury, tellurium, chromium, and arsenic resistance. Of the 34 Exiguobacterium genomes that were inspected, only isolates SH31 and S17 encode the arsenic efflux pump Acr3. Strain SH31 was able to grow in up to 10 mM arsenite and 100 mM arsenate, indicating that it is arsenic resistant. Further, expression of the ars operon and acr3 was strongly induced in response to both toxics, suggesting that the arsenic efflux pump Acr3 mediates arsenic resistance in Exiguobacterium sp. SH31.

Idioma originalEnglish
Número de artículo456
PublicaciónFrontiers in Microbiology
Volumen8
N.ºMAR
DOI
EstadoPublished - 21 mar 2017

Huella dactilar

Arsenic
Genomics
Tellurium
Genes
Lakes
Copper
Genome
Poisons
Chromium
Operon
Heavy Metals
Mercury
Cadmium
Ecosystem
Nucleotides
Salts
Proteins

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Citar esto

Castro-Severyn, Juan ; Remonsellez, Francisco ; Valenzuela, Sandro L. ; Salinas, Cesar ; Fortt, Jonathan ; Aguilar, Pablo ; Pardo-Esté, Coral ; Dorador, Cristina ; Quatrini, Raquel ; Molina, Franck ; Aguayo, Daniel ; Castro-Nallar, Eduardo ; Saavedra, Claudia P. / Comparative genomics analysis of a new Exiguobacterium strain from salar de huasco reveals a repertoire of stress-related genes and arsenic resistance. En: Frontiers in Microbiology. 2017 ; Vol. 8, N.º MAR.
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abstract = "The Atacama Desert hosts diverse ecosystems including salt flats and shallow Andean lakes. Several heavy metals are found in the Atacama Desert, and microorganisms growing in this environment show varying levels of resistance/tolerance to copper, tellurium, and arsenic, among others. Herein, we report the genome sequence and comparative genomic analysis of a new Exiguobacterium strain, sp. SH31, isolated from an altiplanic shallow athalassohaline lake. Exiguobacterium sp. SH31 belongs to the phylogenetic Group II and its closest relative is Exiguobacterium sp. S17, isolated from the Argentinian Altiplano (95{\%} average nucleotide identity). Strain SH31 encodes a wide repertoire of proteins required for cadmium, copper, mercury, tellurium, chromium, and arsenic resistance. Of the 34 Exiguobacterium genomes that were inspected, only isolates SH31 and S17 encode the arsenic efflux pump Acr3. Strain SH31 was able to grow in up to 10 mM arsenite and 100 mM arsenate, indicating that it is arsenic resistant. Further, expression of the ars operon and acr3 was strongly induced in response to both toxics, suggesting that the arsenic efflux pump Acr3 mediates arsenic resistance in Exiguobacterium sp. SH31.",
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Comparative genomics analysis of a new Exiguobacterium strain from salar de huasco reveals a repertoire of stress-related genes and arsenic resistance. / Castro-Severyn, Juan; Remonsellez, Francisco; Valenzuela, Sandro L.; Salinas, Cesar; Fortt, Jonathan; Aguilar, Pablo; Pardo-Esté, Coral; Dorador, Cristina; Quatrini, Raquel; Molina, Franck; Aguayo, Daniel; Castro-Nallar, Eduardo; Saavedra, Claudia P.

En: Frontiers in Microbiology, Vol. 8, N.º MAR, 456, 21.03.2017.

Resultado de la investigación: Article

TY - JOUR

T1 - Comparative genomics analysis of a new Exiguobacterium strain from salar de huasco reveals a repertoire of stress-related genes and arsenic resistance

AU - Castro-Severyn, Juan

AU - Remonsellez, Francisco

AU - Valenzuela, Sandro L.

AU - Salinas, Cesar

AU - Fortt, Jonathan

AU - Aguilar, Pablo

AU - Pardo-Esté, Coral

AU - Dorador, Cristina

AU - Quatrini, Raquel

AU - Molina, Franck

AU - Aguayo, Daniel

AU - Castro-Nallar, Eduardo

AU - Saavedra, Claudia P.

PY - 2017/3/21

Y1 - 2017/3/21

N2 - The Atacama Desert hosts diverse ecosystems including salt flats and shallow Andean lakes. Several heavy metals are found in the Atacama Desert, and microorganisms growing in this environment show varying levels of resistance/tolerance to copper, tellurium, and arsenic, among others. Herein, we report the genome sequence and comparative genomic analysis of a new Exiguobacterium strain, sp. SH31, isolated from an altiplanic shallow athalassohaline lake. Exiguobacterium sp. SH31 belongs to the phylogenetic Group II and its closest relative is Exiguobacterium sp. S17, isolated from the Argentinian Altiplano (95% average nucleotide identity). Strain SH31 encodes a wide repertoire of proteins required for cadmium, copper, mercury, tellurium, chromium, and arsenic resistance. Of the 34 Exiguobacterium genomes that were inspected, only isolates SH31 and S17 encode the arsenic efflux pump Acr3. Strain SH31 was able to grow in up to 10 mM arsenite and 100 mM arsenate, indicating that it is arsenic resistant. Further, expression of the ars operon and acr3 was strongly induced in response to both toxics, suggesting that the arsenic efflux pump Acr3 mediates arsenic resistance in Exiguobacterium sp. SH31.

AB - The Atacama Desert hosts diverse ecosystems including salt flats and shallow Andean lakes. Several heavy metals are found in the Atacama Desert, and microorganisms growing in this environment show varying levels of resistance/tolerance to copper, tellurium, and arsenic, among others. Herein, we report the genome sequence and comparative genomic analysis of a new Exiguobacterium strain, sp. SH31, isolated from an altiplanic shallow athalassohaline lake. Exiguobacterium sp. SH31 belongs to the phylogenetic Group II and its closest relative is Exiguobacterium sp. S17, isolated from the Argentinian Altiplano (95% average nucleotide identity). Strain SH31 encodes a wide repertoire of proteins required for cadmium, copper, mercury, tellurium, chromium, and arsenic resistance. Of the 34 Exiguobacterium genomes that were inspected, only isolates SH31 and S17 encode the arsenic efflux pump Acr3. Strain SH31 was able to grow in up to 10 mM arsenite and 100 mM arsenate, indicating that it is arsenic resistant. Further, expression of the ars operon and acr3 was strongly induced in response to both toxics, suggesting that the arsenic efflux pump Acr3 mediates arsenic resistance in Exiguobacterium sp. SH31.

KW - Chilean Altiplano

KW - Comparative genomics

KW - Exiguobacterium

KW - Polyextremophile

KW - Stress

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M3 - Article

VL - 8

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

IS - MAR

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ER -