Comparative genomic and morphological analyses of Listeria phages isolated from farm environments

Thomas Denes, Kitiya Vongkamjan, Hans Wolfgang Ackermann, Andrea I. Moreno Switt, Martin Wiedmann, Henk C. den Bakker

Resultado de la investigación: Article

25 Citas (Scopus)

Resumen

The genus Listeria is ubiquitous in the environment and includes the globally important food-borne pathogen Listeria monocytogenes. While the genomic diversity of Listeria has been well studied, considerably less is known about the genomic and morphological diversity of Listeria bacteriophages. In this study, we sequenced and analyzed the genomes of 14 Listeria phages isolated mostly from New York dairy farm environments as well as one related Enterococcus faecalis phage to obtain information on genome characteristics and diversity. We also examined 12 of the phages by electron microscopy to characterize their morphology. These Listeria phages, based on gene orthology and morphology, together with previously sequenced Listeria phages could be classified into five orthoclusters, including one novel orthocluster. One orthocluster (orthocluster I) consists of largegenome (̃135-kb) myoviruses belonging to the genus "Twort-like viruses," three orthoclusters (orthoclusters II to IV) contain small-genome (36- to 43-kb) siphoviruses with icosahedral heads, and the novel orthocluster V contains medium-sized-genome (̃66-kb) siphoviruses with elongated heads. A novel orthocluster (orthocluster VI) of E. faecalis phages, with medium-sized genomes (̃56 kb), was identified, which grouped together and shares morphological features with the novel Listeria phage orthocluster V. This new group of phages (i.e., orthoclusters V and VI) is composed of putative lytic phages that may prove to be useful in phage-based applications for biocontrol, detection, and therapeutic purposes.

Idioma originalEnglish
Páginas (desde-hasta)4616-4625
Número de páginas10
PublicaciónApplied and Environmental Microbiology
Volumen80
N.º15
DOI
EstadoPublished - 2014

Huella dactilar

Listeria
bacteriophages
Bacteriophages
genomics
genome
farm
farms
Genome
bacteriophage
Enterococcus faecalis
electron microscopy
virus
pathogen
Farms
Head
food
gene
Listeria monocytogenes
food pathogens
dairy farming

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology
  • Medicine(all)

Citar esto

Denes, Thomas ; Vongkamjan, Kitiya ; Ackermann, Hans Wolfgang ; Moreno Switt, Andrea I. ; Wiedmann, Martin ; den Bakker, Henk C. / Comparative genomic and morphological analyses of Listeria phages isolated from farm environments. En: Applied and Environmental Microbiology. 2014 ; Vol. 80, N.º 15. pp. 4616-4625.
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Comparative genomic and morphological analyses of Listeria phages isolated from farm environments. / Denes, Thomas; Vongkamjan, Kitiya; Ackermann, Hans Wolfgang; Moreno Switt, Andrea I.; Wiedmann, Martin; den Bakker, Henk C.

En: Applied and Environmental Microbiology, Vol. 80, N.º 15, 2014, p. 4616-4625.

Resultado de la investigación: Article

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AU - Denes, Thomas

AU - Vongkamjan, Kitiya

AU - Ackermann, Hans Wolfgang

AU - Moreno Switt, Andrea I.

AU - Wiedmann, Martin

AU - den Bakker, Henk C.

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