Plant species in sensu stricto Cerrado have adaptive strategies to soil and climatic adversities that require high investment of nutrients, water and photoassimilates. The mutualistic fungi - plant root symbiosis is an important adaptation by which plants can improve soil nutrients and water acquisition and it can he determinant for plant survival in Cerrado conditions. The aim of this study was to establish a method for studying arbuscular mycorrhizal fungi (AMF) and. dark septate endophytic fungi. (DSEF) in. roots of some tree and. shrub species in. stricto sensu Cerrado. The best method for root clarification was obtained after autoclaving root samples with. 2 % KOH for 20 min followed, by incubation in a new 2 % KOH solution for 24 hat room temperature. This procedure was repeated once and subsequently the samples were immersed in 2 % H 2O2for 2 h. The arbuscules were thoroughly examined after resin inclusion, sectioned and stained with toluidine blue. All plant species evaluated were associated to AMF and all but Xylopia aromatica were associated to DSEF as well. The mycorrhizal frequency in shrubs was higher than in tree species. The widespread occurrence of AMF and DSEF symbioses suggests that they represent an important adaptation, to environmental factors in the Cerrado region.
|Título traducido de la contribución||Comparision of methods to visualise arbuscular mycorrhizal and dark septate endophytic fungi in native cerrado species|
|Número de páginas||8|
|Publicación||Revista Brasileira de Ciencia do Solo|
|Estado||Publicada - sep 2008|
- Mycorrhizal colonization
Áreas temáticas de ASJC Scopus
- Agronomía y cultivos