Comparação de métodos para a observação de fungos micorrízicos arbusculares e endofíticgs do tipo dark septate em espécies nativas de cerrado

Kelly Da Silva Coutinho Detmann, Marina Meves Delgado, Vitor Paiva Alcoforado Rebello, Tiago De Souza Leite, Aristéa Alves Azevedo, Maria Catarina Megumi Kasuya, Andrea Miyasaka De Almeida

Resultado de la investigación: Article

9 Citas (Scopus)

Resumen

Plant species in sensu stricto Cerrado have adaptive strategies to soil and climatic adversities that require high investment of nutrients, water and photoassimilates. The mutualistic fungi - plant root symbiosis is an important adaptation by which plants can improve soil nutrients and water acquisition and it can he determinant for plant survival in Cerrado conditions. The aim of this study was to establish a method for studying arbuscular mycorrhizal fungi (AMF) and. dark septate endophytic fungi. (DSEF) in. roots of some tree and. shrub species in. stricto sensu Cerrado. The best method for root clarification was obtained after autoclaving root samples with. 2 % KOH for 20 min followed, by incubation in a new 2 % KOH solution for 24 hat room temperature. This procedure was repeated once and subsequently the samples were immersed in 2 % H 2O2for 2 h. The arbuscules were thoroughly examined after resin inclusion, sectioned and stained with toluidine blue. All plant species evaluated were associated to AMF and all but Xylopia aromatica were associated to DSEF as well. The mycorrhizal frequency in shrubs was higher than in tree species. The widespread occurrence of AMF and DSEF symbioses suggests that they represent an important adaptation, to environmental factors in the Cerrado region.

Idioma originalPortuguese
Páginas (desde-hasta)1883-1890
Número de páginas8
PublicaciónRevista Brasileira de Ciencia do Solo
Volumen32
N.º5
EstadoPublished - sep 2008

Huella dactilar

cerrado
fungus
fungi
mycorrhizal fungi
symbiosis
shrub
shrubs
Xylopia
toluidine blue
methodology
autoclaving
soil nutrient
soil nutrients
resins
resin
ambient temperature
environmental factor
soil water
incubation
method

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Soil Science

Citar esto

Da Silva Coutinho Detmann, K., Delgado, M. M., Rebello, V. P. A., De Souza Leite, T., Azevedo, A. A., Kasuya, M. C. M., & De Almeida, A. M. (2008). Comparação de métodos para a observação de fungos micorrízicos arbusculares e endofíticgs do tipo dark septate em espécies nativas de cerrado. Revista Brasileira de Ciencia do Solo, 32(5), 1883-1890.
Da Silva Coutinho Detmann, Kelly ; Delgado, Marina Meves ; Rebello, Vitor Paiva Alcoforado ; De Souza Leite, Tiago ; Azevedo, Aristéa Alves ; Kasuya, Maria Catarina Megumi ; De Almeida, Andrea Miyasaka. / Comparação de métodos para a observação de fungos micorrízicos arbusculares e endofíticgs do tipo dark septate em espécies nativas de cerrado. En: Revista Brasileira de Ciencia do Solo. 2008 ; Vol. 32, N.º 5. pp. 1883-1890.
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title = "Compara{\cc}{\~a}o de m{\'e}todos para a observa{\cc}{\~a}o de fungos micorr{\'i}zicos arbusculares e endof{\'i}ticgs do tipo dark septate em esp{\'e}cies nativas de cerrado",
abstract = "Plant species in sensu stricto Cerrado have adaptive strategies to soil and climatic adversities that require high investment of nutrients, water and photoassimilates. The mutualistic fungi - plant root symbiosis is an important adaptation by which plants can improve soil nutrients and water acquisition and it can he determinant for plant survival in Cerrado conditions. The aim of this study was to establish a method for studying arbuscular mycorrhizal fungi (AMF) and. dark septate endophytic fungi. (DSEF) in. roots of some tree and. shrub species in. stricto sensu Cerrado. The best method for root clarification was obtained after autoclaving root samples with. 2 {\%} KOH for 20 min followed, by incubation in a new 2 {\%} KOH solution for 24 hat room temperature. This procedure was repeated once and subsequently the samples were immersed in 2 {\%} H 2O2for 2 h. The arbuscules were thoroughly examined after resin inclusion, sectioned and stained with toluidine blue. All plant species evaluated were associated to AMF and all but Xylopia aromatica were associated to DSEF as well. The mycorrhizal frequency in shrubs was higher than in tree species. The widespread occurrence of AMF and DSEF symbioses suggests that they represent an important adaptation, to environmental factors in the Cerrado region.",
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author = "{Da Silva Coutinho Detmann}, Kelly and Delgado, {Marina Meves} and Rebello, {Vitor Paiva Alcoforado} and {De Souza Leite}, Tiago and Azevedo, {Arist{\'e}a Alves} and Kasuya, {Maria Catarina Megumi} and {De Almeida}, {Andrea Miyasaka}",
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Da Silva Coutinho Detmann, K, Delgado, MM, Rebello, VPA, De Souza Leite, T, Azevedo, AA, Kasuya, MCM & De Almeida, AM 2008, 'Comparação de métodos para a observação de fungos micorrízicos arbusculares e endofíticgs do tipo dark septate em espécies nativas de cerrado', Revista Brasileira de Ciencia do Solo, vol. 32, n.º 5, pp. 1883-1890.

Comparação de métodos para a observação de fungos micorrízicos arbusculares e endofíticgs do tipo dark septate em espécies nativas de cerrado. / Da Silva Coutinho Detmann, Kelly; Delgado, Marina Meves; Rebello, Vitor Paiva Alcoforado; De Souza Leite, Tiago; Azevedo, Aristéa Alves; Kasuya, Maria Catarina Megumi; De Almeida, Andrea Miyasaka.

En: Revista Brasileira de Ciencia do Solo, Vol. 32, N.º 5, 09.2008, p. 1883-1890.

Resultado de la investigación: Article

TY - JOUR

T1 - Comparação de métodos para a observação de fungos micorrízicos arbusculares e endofíticgs do tipo dark septate em espécies nativas de cerrado

AU - Da Silva Coutinho Detmann, Kelly

AU - Delgado, Marina Meves

AU - Rebello, Vitor Paiva Alcoforado

AU - De Souza Leite, Tiago

AU - Azevedo, Aristéa Alves

AU - Kasuya, Maria Catarina Megumi

AU - De Almeida, Andrea Miyasaka

PY - 2008/9

Y1 - 2008/9

N2 - Plant species in sensu stricto Cerrado have adaptive strategies to soil and climatic adversities that require high investment of nutrients, water and photoassimilates. The mutualistic fungi - plant root symbiosis is an important adaptation by which plants can improve soil nutrients and water acquisition and it can he determinant for plant survival in Cerrado conditions. The aim of this study was to establish a method for studying arbuscular mycorrhizal fungi (AMF) and. dark septate endophytic fungi. (DSEF) in. roots of some tree and. shrub species in. stricto sensu Cerrado. The best method for root clarification was obtained after autoclaving root samples with. 2 % KOH for 20 min followed, by incubation in a new 2 % KOH solution for 24 hat room temperature. This procedure was repeated once and subsequently the samples were immersed in 2 % H 2O2for 2 h. The arbuscules were thoroughly examined after resin inclusion, sectioned and stained with toluidine blue. All plant species evaluated were associated to AMF and all but Xylopia aromatica were associated to DSEF as well. The mycorrhizal frequency in shrubs was higher than in tree species. The widespread occurrence of AMF and DSEF symbioses suggests that they represent an important adaptation, to environmental factors in the Cerrado region.

AB - Plant species in sensu stricto Cerrado have adaptive strategies to soil and climatic adversities that require high investment of nutrients, water and photoassimilates. The mutualistic fungi - plant root symbiosis is an important adaptation by which plants can improve soil nutrients and water acquisition and it can he determinant for plant survival in Cerrado conditions. The aim of this study was to establish a method for studying arbuscular mycorrhizal fungi (AMF) and. dark septate endophytic fungi. (DSEF) in. roots of some tree and. shrub species in. stricto sensu Cerrado. The best method for root clarification was obtained after autoclaving root samples with. 2 % KOH for 20 min followed, by incubation in a new 2 % KOH solution for 24 hat room temperature. This procedure was repeated once and subsequently the samples were immersed in 2 % H 2O2for 2 h. The arbuscules were thoroughly examined after resin inclusion, sectioned and stained with toluidine blue. All plant species evaluated were associated to AMF and all but Xylopia aromatica were associated to DSEF as well. The mycorrhizal frequency in shrubs was higher than in tree species. The widespread occurrence of AMF and DSEF symbioses suggests that they represent an important adaptation, to environmental factors in the Cerrado region.

KW - AMF

KW - DSEF

KW - Mycorrhizal colonization

KW - Shrub

KW - Tree

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M3 - Article

AN - SCOPUS:64249148845

VL - 32

SP - 1883

EP - 1890

JO - Revista Brasileira de Ciencia do Solo

JF - Revista Brasileira de Ciencia do Solo

SN - 0100-0683

IS - 5

ER -

Da Silva Coutinho Detmann K, Delgado MM, Rebello VPA, De Souza Leite T, Azevedo AA, Kasuya MCM y otros. Comparação de métodos para a observação de fungos micorrízicos arbusculares e endofíticgs do tipo dark septate em espécies nativas de cerrado. Revista Brasileira de Ciencia do Solo. 2008 sep;32(5):1883-1890.