Comparação de métodos para a observação de fungos micorrízicos arbusculares e endofíticgs do tipo dark septate em espécies nativas de cerrado

Kelly Da Silva Coutinho Detmann, Marina Meves Delgado, Vitor Paiva Alcoforado Rebello, Tiago De Souza Leite, Aristéa Alves Azevedo, Maria Catarina Megumi Kasuya, Andrea Miyasaka De Almeida

Resultado de la investigación: Contribución a una revistaArtículo

10 Citas (Scopus)

Resumen

Plant species in sensu stricto Cerrado have adaptive strategies to soil and climatic adversities that require high investment of nutrients, water and photoassimilates. The mutualistic fungi - plant root symbiosis is an important adaptation by which plants can improve soil nutrients and water acquisition and it can he determinant for plant survival in Cerrado conditions. The aim of this study was to establish a method for studying arbuscular mycorrhizal fungi (AMF) and. dark septate endophytic fungi. (DSEF) in. roots of some tree and. shrub species in. stricto sensu Cerrado. The best method for root clarification was obtained after autoclaving root samples with. 2 % KOH for 20 min followed, by incubation in a new 2 % KOH solution for 24 hat room temperature. This procedure was repeated once and subsequently the samples were immersed in 2 % H 2O2for 2 h. The arbuscules were thoroughly examined after resin inclusion, sectioned and stained with toluidine blue. All plant species evaluated were associated to AMF and all but Xylopia aromatica were associated to DSEF as well. The mycorrhizal frequency in shrubs was higher than in tree species. The widespread occurrence of AMF and DSEF symbioses suggests that they represent an important adaptation, to environmental factors in the Cerrado region.

Título traducido de la contribuciónComparision of methods to visualise arbuscular mycorrhizal and dark septate endophytic fungi in native cerrado species
Idioma originalPortugués
Páginas (desde-hasta)1883-1890
Número de páginas8
PublicaciónRevista Brasileira de Ciencia do Solo
Volumen32
N.º5
EstadoPublicada - sep 2008

Palabras clave

  • AMF
  • DSEF
  • Mycorrhizal colonization
  • Shrub
  • Tree

Áreas temáticas de ASJC Scopus

  • Agronomía y cultivos
  • Edafología

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  • Citar esto

    Da Silva Coutinho Detmann, K., Delgado, M. M., Rebello, V. P. A., De Souza Leite, T., Azevedo, A. A., Kasuya, M. C. M., & De Almeida, A. M. (2008). Comparação de métodos para a observação de fungos micorrízicos arbusculares e endofíticgs do tipo dark septate em espécies nativas de cerrado. Revista Brasileira de Ciencia do Solo, 32(5), 1883-1890.