Co-synthesis of medium-chain-length polyhydroxyalkanoates and CdS quantum dots nanoparticles in Pseudomonas putida KT2440

Barbara Oliva-Arancibia, Nicolás Órdenes-Aenishanslins, Nicolas Bruna, Paula S. Ibarra, Flavia C. Zacconi, José M. Pérez-Donoso, Ignacio Poblete-Castro

Resultado de la investigación: Article

7 Citas (Scopus)

Resumen

Microbial polymers and nanomaterials production is a promising alternative for sustainable bioeconomics. To this end, we used Pseudomonas putida KT2440 as a cell factory in batch cultures to coproduce two important nanotechnology materials– medium-chain-length (MCL)-polyhydroxyalkanoates (PHAs) and CdS fluorescent nanoparticles (i.e. quantum dots [QDots]). Due to high cadmium resistance, biomass and PHA yields were almost unaffected by coproduction conditions. Fluorescent nanocrystal biosynthesis was possible only in presence of cysteine. Furthermore, this process took place exclusively in the cell, displaying the classical emission spectra of CdS QDots under UV-light exposure. Cell fluorescence, zeta potential values, and particles size of QDots depended on cadmium concentration and exposure time. Using standard PHA-extraction procedures, the biosynthesized QDots remained associated with the biomass, and the resulting PHAs presented no traces of CdS QDots. Transmission electron microscopy located the synthesized PHAs in the cell cytoplasm, whereas CdS nanocrystals were most likely located within the periplasmic space, exhibiting no apparent interaction. This is the first report presenting the microbial coproduction of MCL-PHAs and CdS QDots in P. putida KT2440, thus constituting a foundation for further bioprocess developments and strain engineering towards the efficient synthesis of these highly relevant bioproducts for nanotechnology.

Idioma originalEnglish
Páginas (desde-hasta)29-37
Número de páginas9
PublicaciónJournal of Biotechnology
Volumen264
DOI
EstadoPublished - 20 dic 2017

Huella dactilar

Polyhydroxyalkanoates
Quantum Dots
Pseudomonas putida
Chain length
Nanoparticles
Semiconductor quantum dots
Nanotechnology
Cadmium
Biomass
Nanocrystals
Periplasm
Batch Cell Culture Techniques
Nanostructures
Biosynthesis
Zeta potential
Ultraviolet Rays
Transmission Electron Microscopy
Particle Size
Nanostructured materials
Ultraviolet radiation

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Citar esto

Oliva-Arancibia, Barbara ; Órdenes-Aenishanslins, Nicolás ; Bruna, Nicolas ; Ibarra, Paula S. ; Zacconi, Flavia C. ; Pérez-Donoso, José M. ; Poblete-Castro, Ignacio. / Co-synthesis of medium-chain-length polyhydroxyalkanoates and CdS quantum dots nanoparticles in Pseudomonas putida KT2440. En: Journal of Biotechnology. 2017 ; Vol. 264. pp. 29-37.
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title = "Co-synthesis of medium-chain-length polyhydroxyalkanoates and CdS quantum dots nanoparticles in Pseudomonas putida KT2440",
abstract = "Microbial polymers and nanomaterials production is a promising alternative for sustainable bioeconomics. To this end, we used Pseudomonas putida KT2440 as a cell factory in batch cultures to coproduce two important nanotechnology materials– medium-chain-length (MCL)-polyhydroxyalkanoates (PHAs) and CdS fluorescent nanoparticles (i.e. quantum dots [QDots]). Due to high cadmium resistance, biomass and PHA yields were almost unaffected by coproduction conditions. Fluorescent nanocrystal biosynthesis was possible only in presence of cysteine. Furthermore, this process took place exclusively in the cell, displaying the classical emission spectra of CdS QDots under UV-light exposure. Cell fluorescence, zeta potential values, and particles size of QDots depended on cadmium concentration and exposure time. Using standard PHA-extraction procedures, the biosynthesized QDots remained associated with the biomass, and the resulting PHAs presented no traces of CdS QDots. Transmission electron microscopy located the synthesized PHAs in the cell cytoplasm, whereas CdS nanocrystals were most likely located within the periplasmic space, exhibiting no apparent interaction. This is the first report presenting the microbial coproduction of MCL-PHAs and CdS QDots in P. putida KT2440, thus constituting a foundation for further bioprocess developments and strain engineering towards the efficient synthesis of these highly relevant bioproducts for nanotechnology.",
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year = "2017",
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Co-synthesis of medium-chain-length polyhydroxyalkanoates and CdS quantum dots nanoparticles in Pseudomonas putida KT2440. / Oliva-Arancibia, Barbara; Órdenes-Aenishanslins, Nicolás; Bruna, Nicolas; Ibarra, Paula S.; Zacconi, Flavia C.; Pérez-Donoso, José M.; Poblete-Castro, Ignacio.

En: Journal of Biotechnology, Vol. 264, 20.12.2017, p. 29-37.

Resultado de la investigación: Article

TY - JOUR

T1 - Co-synthesis of medium-chain-length polyhydroxyalkanoates and CdS quantum dots nanoparticles in Pseudomonas putida KT2440

AU - Oliva-Arancibia, Barbara

AU - Órdenes-Aenishanslins, Nicolás

AU - Bruna, Nicolas

AU - Ibarra, Paula S.

AU - Zacconi, Flavia C.

AU - Pérez-Donoso, José M.

AU - Poblete-Castro, Ignacio

PY - 2017/12/20

Y1 - 2017/12/20

N2 - Microbial polymers and nanomaterials production is a promising alternative for sustainable bioeconomics. To this end, we used Pseudomonas putida KT2440 as a cell factory in batch cultures to coproduce two important nanotechnology materials– medium-chain-length (MCL)-polyhydroxyalkanoates (PHAs) and CdS fluorescent nanoparticles (i.e. quantum dots [QDots]). Due to high cadmium resistance, biomass and PHA yields were almost unaffected by coproduction conditions. Fluorescent nanocrystal biosynthesis was possible only in presence of cysteine. Furthermore, this process took place exclusively in the cell, displaying the classical emission spectra of CdS QDots under UV-light exposure. Cell fluorescence, zeta potential values, and particles size of QDots depended on cadmium concentration and exposure time. Using standard PHA-extraction procedures, the biosynthesized QDots remained associated with the biomass, and the resulting PHAs presented no traces of CdS QDots. Transmission electron microscopy located the synthesized PHAs in the cell cytoplasm, whereas CdS nanocrystals were most likely located within the periplasmic space, exhibiting no apparent interaction. This is the first report presenting the microbial coproduction of MCL-PHAs and CdS QDots in P. putida KT2440, thus constituting a foundation for further bioprocess developments and strain engineering towards the efficient synthesis of these highly relevant bioproducts for nanotechnology.

AB - Microbial polymers and nanomaterials production is a promising alternative for sustainable bioeconomics. To this end, we used Pseudomonas putida KT2440 as a cell factory in batch cultures to coproduce two important nanotechnology materials– medium-chain-length (MCL)-polyhydroxyalkanoates (PHAs) and CdS fluorescent nanoparticles (i.e. quantum dots [QDots]). Due to high cadmium resistance, biomass and PHA yields were almost unaffected by coproduction conditions. Fluorescent nanocrystal biosynthesis was possible only in presence of cysteine. Furthermore, this process took place exclusively in the cell, displaying the classical emission spectra of CdS QDots under UV-light exposure. Cell fluorescence, zeta potential values, and particles size of QDots depended on cadmium concentration and exposure time. Using standard PHA-extraction procedures, the biosynthesized QDots remained associated with the biomass, and the resulting PHAs presented no traces of CdS QDots. Transmission electron microscopy located the synthesized PHAs in the cell cytoplasm, whereas CdS nanocrystals were most likely located within the periplasmic space, exhibiting no apparent interaction. This is the first report presenting the microbial coproduction of MCL-PHAs and CdS QDots in P. putida KT2440, thus constituting a foundation for further bioprocess developments and strain engineering towards the efficient synthesis of these highly relevant bioproducts for nanotechnology.

KW - CdS quantum dots

KW - Coproduction

KW - MCL-Polyhydroxyalkanoates

KW - Pseudomonas putida

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DO - 10.1016/j.jbiotec.2017.10.013

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AN - SCOPUS:85032273750

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SP - 29

EP - 37

JO - Journal of Biotechnology

JF - Journal of Biotechnology

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