Cloning, physical mapping and genome organization of mitochondrial DNA from Cyprinus carpio oocytes

Alejandro Araya, Rodolfo Amthauer, Gloria Leon, Manuel Krauskopf

Resultado de la investigación: Article

12 Citas (Scopus)

Resumen

The mitochondrial genome from Cyprinus carpio oocytes is a 10.5 megadalton, circular DNA molecule. The carp mitochondrial DNA was cloned in pBR325. Three recombinant plasmids accounted for the entire genome. Mapping of this DNA using 11 different restriction endonucleases is reported here. Both the large and small rRNA genes were then localized using Southern blot analysis. The subunit I of the cytochrome oxidase, the cytochrome b, the tRNAGlu and the URF 4 genes were localized by nucleotide sequence analysis and homology studies with human mtDNA. Our results suggest that a similar gene order has been maintained in the mitochondrial genomes of Chordata and support the hypothesis of a common ancestor for all vertebrate organelle genomes. This study constitutes the first report on the genome organization of a fish mtDNA and provides information for further investigation in connection with sequence determination, replication, and gene expression in carp mitochondria.

Idioma originalEnglish
Páginas (desde-hasta)43-52
Número de páginas10
PublicaciónMGG Molecular & General Genetics
Volumen196
N.º1
DOI
EstadoPublished - ago 1984

Huella dactilar

Carps
Chromosome Mapping
Mitochondrial DNA
Oocytes
Organism Cloning
Mitochondrial Genome
Genome
Sequence Analysis
RNA, Transfer, Glu
Chordata
Circular DNA
Cytochromes b
Gene Order
DNA Restriction Enzymes
Electron Transport Complex IV
Sequence Homology
Southern Blotting
rRNA Genes
Organelles
Vertebrates

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Citar esto

Araya, Alejandro ; Amthauer, Rodolfo ; Leon, Gloria ; Krauskopf, Manuel. / Cloning, physical mapping and genome organization of mitochondrial DNA from Cyprinus carpio oocytes. En: MGG Molecular & General Genetics. 1984 ; Vol. 196, N.º 1. pp. 43-52.
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Cloning, physical mapping and genome organization of mitochondrial DNA from Cyprinus carpio oocytes. / Araya, Alejandro; Amthauer, Rodolfo; Leon, Gloria; Krauskopf, Manuel.

En: MGG Molecular & General Genetics, Vol. 196, N.º 1, 08.1984, p. 43-52.

Resultado de la investigación: Article

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AU - Krauskopf, Manuel

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