Cloning, expression and characterization of the 36 KDal Salmonella typhi porin gene in Escherichia coli

J. Agüero; G. Mora; M. J. Mroczenski-Wildey; M. E. Fernandez-Beros; L. Aron; F. C. Cabello

doi:10.1016/0882-4010(87)90010-6

Cloning, expression and characterization of the 36 KDal Salmonella typhi porin gene in Escherichia coli

J. Agüero, G. Mora, M. J. Mroczenski-Wildey, M. E. Fernandez-Beros, L. Aron, F. C. Cabello

Facultad Medicina

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Resumen

A recombinant plasmid containing the gene for the 36 KDal porin of Salmonella typhi has been identified in a cosmid library of S. typhi propagated in Escherichia coli. The recombinant clone was identified by its ability to endow E. coli with susceptibility to porin specific phages, and by the appearance in the outer membrane of E. coli containing the clone of a new protein of 36 KDal. While the porin confers upon a porinless mutant of E. coli an increased susceptibility to β-lactam antibiotics, it does not react with serum from patients with typhoid fever in immunoblotting assays.

Idioma original	Inglés
Páginas (desde-hasta)	399-407
Número de páginas	9
Publicación	Microbial Pathogenesis
Volumen	3
N.º	6
DOI	https://doi.org/10.1016/0882-4010(87)90010-6
Estado	Publicada - 1 ene. 1987

Áreas temáticas de ASJC Scopus

Microbiología
Enfermedades infecciosas

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title = "Cloning, expression and characterization of the 36 KDal Salmonella typhi porin gene in Escherichia coli",

abstract = "A recombinant plasmid containing the gene for the 36 KDal porin of Salmonella typhi has been identified in a cosmid library of S. typhi propagated in Escherichia coli. The recombinant clone was identified by its ability to endow E. coli with susceptibility to porin specific phages, and by the appearance in the outer membrane of E. coli containing the clone of a new protein of 36 KDal. While the porin confers upon a porinless mutant of E. coli an increased susceptibility to β-lactam antibiotics, it does not react with serum from patients with typhoid fever in immunoblotting assays.",

keywords = "36 KDal porin, cloning in E. coli, immunoblotting, Salmonella typhi, typhoid fever",

author = "J. Ag{\"u}ero and G. Mora and Mroczenski-Wildey, {M. J.} and Fernandez-Beros, {M. E.} and L. Aron and Cabello, {F. C.}",

year = "1987",

month = jan,

day = "1",

doi = "10.1016/0882-4010(87)90010-6",

language = "English",

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pages = "399--407",

journal = "Microbial Pathogenesis",

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publisher = "Academic Press Inc.",

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TY - JOUR

T1 - Cloning, expression and characterization of the 36 KDal Salmonella typhi porin gene in Escherichia coli

AU - Agüero, J.

AU - Mora, G.

AU - Mroczenski-Wildey, M. J.

AU - Fernandez-Beros, M. E.

AU - Aron, L.

AU - Cabello, F. C.

PY - 1987/1/1

Y1 - 1987/1/1

N2 - A recombinant plasmid containing the gene for the 36 KDal porin of Salmonella typhi has been identified in a cosmid library of S. typhi propagated in Escherichia coli. The recombinant clone was identified by its ability to endow E. coli with susceptibility to porin specific phages, and by the appearance in the outer membrane of E. coli containing the clone of a new protein of 36 KDal. While the porin confers upon a porinless mutant of E. coli an increased susceptibility to β-lactam antibiotics, it does not react with serum from patients with typhoid fever in immunoblotting assays.

AB - A recombinant plasmid containing the gene for the 36 KDal porin of Salmonella typhi has been identified in a cosmid library of S. typhi propagated in Escherichia coli. The recombinant clone was identified by its ability to endow E. coli with susceptibility to porin specific phages, and by the appearance in the outer membrane of E. coli containing the clone of a new protein of 36 KDal. While the porin confers upon a porinless mutant of E. coli an increased susceptibility to β-lactam antibiotics, it does not react with serum from patients with typhoid fever in immunoblotting assays.

KW - 36 KDal porin

KW - cloning in E. coli

KW - immunoblotting

KW - Salmonella typhi

KW - typhoid fever

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U2 - 10.1016/0882-4010(87)90010-6

DO - 10.1016/0882-4010(87)90010-6

M3 - Article

C2 - 2462154

AN - SCOPUS:0023589349

SN - 0882-4010

VL - 3

SP - 399

EP - 407

JO - Microbial Pathogenesis

JF - Microbial Pathogenesis

IS - 6

ER -

Cloning, expression and characterization of the 36 KDal Salmonella typhi porin gene in Escherichia coli

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