Cloning and expression analysis of an inducible HSP70 gene from tilapia fish

Alfredo Molina, Frédéric Biemar, Ferenc Müller, Arati Iyengar, Patrick Prunet, Norman MacLean, Joseph A. Martial, Marc Muller

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

81 Citas (Scopus)

Resumen

We isolated and characterized the tilapia (Oreochromis mossambicus) HSP70 gene, highly homologous to other HSP70 genes. A dramatic increase of tilapia HSP70 mRNA levels was observed after heat shock of whole animals in all organs tested. Reporter constructs were tested for transient expression in carp cells and in microinjected zebrafish embryos. The entire isolated regulatory region (-851/+157) was able to mediate heat shock inducible expression of the reporter gene, with no preference for a particular tissue. Our studies represent the first transcriptional analysis of a HSP70 promoter from fish, revealing a powerful tool to direct controlled, tissue-independent gene expression in fish. Copyright (C) 2000 Federation of European Biochemical Societies.

Idioma originalInglés
Páginas (desde-hasta)5-10
Número de páginas6
PublicaciónFEBS Letters
Volumen474
N.º1
DOI
EstadoPublicada - 26 may 2000

Áreas temáticas de ASJC Scopus

  • Biofísica
  • Biología estructural
  • Bioquímica
  • Biología molecular
  • Genética
  • Biología celular

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