@article{cb832bfa1c1e443b9f431cac71a7950d,
title = "Cloning and expression analysis of an inducible HSP70 gene from tilapia fish",
abstract = "We isolated and characterized the tilapia (Oreochromis mossambicus) HSP70 gene, highly homologous to other HSP70 genes. A dramatic increase of tilapia HSP70 mRNA levels was observed after heat shock of whole animals in all organs tested. Reporter constructs were tested for transient expression in carp cells and in microinjected zebrafish embryos. The entire isolated regulatory region (-851/+157) was able to mediate heat shock inducible expression of the reporter gene, with no preference for a particular tissue. Our studies represent the first transcriptional analysis of a HSP70 promoter from fish, revealing a powerful tool to direct controlled, tissue-independent gene expression in fish. Copyright (C) 2000 Federation of European Biochemical Societies.",
keywords = "Expression, HSP70, Heat shock, Tilapia, Zebrafish",
author = "Alfredo Molina and Fr{\'e}d{\'e}ric Biemar and Ferenc M{\"u}ller and Arati Iyengar and Patrick Prunet and Norman MacLean and Martial, {Joseph A.} and Marc Muller",
note = "Funding Information: We are grateful to S.E. Wendelaar Bonga (Nijmegen, The Netherlands) for providing fish material. We would like to thank R. Carpeaux for excellent technical assistance. This work was supported by the {\textquoteleft}R{\'e}gion Wallone{\textquoteright} (ULg 1815); the {\textquoteleft}Services F{\'e}d{\'e}raux des Affaires Scientifiques, Techniques et Culturelles{\textquoteright} (PAI P4/30 and {\textquoteleft}Actions de Recherche Concert{\'e}es{\textquoteright}: 95/00-193); the Fonds National de la Recherche Scientifique (FNRS) (−3.4537.93 and −9.4569.95) and the EU (No. BIO4-CT97-0554). M.M. is a {\textquoteleft}Chercheur qualifi{\'e}{\textquoteright} at the FNRS. A.M. held fellowships from {\textquoteleft}CGRI{\textquoteright} and {\textquoteleft}ULg patrimoine{\textquoteright}.",
year = "2000",
month = may,
day = "26",
doi = "10.1016/S0014-5793(00)01538-6",
language = "English",
volume = "474",
pages = "5--10",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Wiley-Blackwell",
number = "1",
}