Cloning and analysis of integrated hepatitis virus sequences from a human hepatoma cell line

Y. Shaul, M. Ziemer, P. D. Garcia, R. Crawford, H. Hsu, P. Valenzuela, W. J. Rutter

Resultado de la investigación: Article

48 Citas (Scopus)

Resumen

We report the isolation by molecular cloning and the analysis by heteroduplex and restriction enzyme mapping of seven distinct DNA fragments containing hepatitis B virus (HBV) sequences from genomic DNA of the PLC/PRF/5 human liver carcinoma cell line (the Alexander cell). No intact full-length HBV genomes were present. Three different patterns of organization of HBV fragments were detected. These included two linear fragments without detectable rearrangement, three other HBV fragments with internal deletions, and two HBV fragments containing long inverted duplications. HBsAg sequences are preferentially included in the integrated fragment, whereas the core gene is preferentially eliminated. Several of the integrated HBV fragments might act as templates for the synthesis of functional HBsAg mRNA, whereas only one clone could produce a full core antigen transcript.

Idioma originalEnglish
Páginas (desde-hasta)776-787
Número de páginas12
PublicaciónJournal of Virology
Volumen51
N.º3
EstadoPublished - 1984

Huella dactilar

Hepatitis Viruses
Hepatitis B virus
hepatitis
hepatoma
Organism Cloning
molecular cloning
Hepatocellular Carcinoma
cell lines
Cell Line
viruses
Hepatitis B Surface Antigens
Heteroduplex Analysis
Restriction Mapping
restriction mapping
Molecular Cloning
DNA
carcinoma
Clone Cells
Genome
clones

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Insect Science
  • Virology

Citar esto

Shaul, Y., Ziemer, M., Garcia, P. D., Crawford, R., Hsu, H., Valenzuela, P., & Rutter, W. J. (1984). Cloning and analysis of integrated hepatitis virus sequences from a human hepatoma cell line. Journal of Virology, 51(3), 776-787.
Shaul, Y. ; Ziemer, M. ; Garcia, P. D. ; Crawford, R. ; Hsu, H. ; Valenzuela, P. ; Rutter, W. J. / Cloning and analysis of integrated hepatitis virus sequences from a human hepatoma cell line. En: Journal of Virology. 1984 ; Vol. 51, N.º 3. pp. 776-787.
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Shaul, Y, Ziemer, M, Garcia, PD, Crawford, R, Hsu, H, Valenzuela, P & Rutter, WJ 1984, 'Cloning and analysis of integrated hepatitis virus sequences from a human hepatoma cell line', Journal of Virology, vol. 51, n.º 3, pp. 776-787.

Cloning and analysis of integrated hepatitis virus sequences from a human hepatoma cell line. / Shaul, Y.; Ziemer, M.; Garcia, P. D.; Crawford, R.; Hsu, H.; Valenzuela, P.; Rutter, W. J.

En: Journal of Virology, Vol. 51, N.º 3, 1984, p. 776-787.

Resultado de la investigación: Article

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T1 - Cloning and analysis of integrated hepatitis virus sequences from a human hepatoma cell line

AU - Shaul, Y.

AU - Ziemer, M.

AU - Garcia, P. D.

AU - Crawford, R.

AU - Hsu, H.

AU - Valenzuela, P.

AU - Rutter, W. J.

PY - 1984

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N2 - We report the isolation by molecular cloning and the analysis by heteroduplex and restriction enzyme mapping of seven distinct DNA fragments containing hepatitis B virus (HBV) sequences from genomic DNA of the PLC/PRF/5 human liver carcinoma cell line (the Alexander cell). No intact full-length HBV genomes were present. Three different patterns of organization of HBV fragments were detected. These included two linear fragments without detectable rearrangement, three other HBV fragments with internal deletions, and two HBV fragments containing long inverted duplications. HBsAg sequences are preferentially included in the integrated fragment, whereas the core gene is preferentially eliminated. Several of the integrated HBV fragments might act as templates for the synthesis of functional HBsAg mRNA, whereas only one clone could produce a full core antigen transcript.

AB - We report the isolation by molecular cloning and the analysis by heteroduplex and restriction enzyme mapping of seven distinct DNA fragments containing hepatitis B virus (HBV) sequences from genomic DNA of the PLC/PRF/5 human liver carcinoma cell line (the Alexander cell). No intact full-length HBV genomes were present. Three different patterns of organization of HBV fragments were detected. These included two linear fragments without detectable rearrangement, three other HBV fragments with internal deletions, and two HBV fragments containing long inverted duplications. HBsAg sequences are preferentially included in the integrated fragment, whereas the core gene is preferentially eliminated. Several of the integrated HBV fragments might act as templates for the synthesis of functional HBsAg mRNA, whereas only one clone could produce a full core antigen transcript.

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Shaul Y, Ziemer M, Garcia PD, Crawford R, Hsu H, Valenzuela P y otros. Cloning and analysis of integrated hepatitis virus sequences from a human hepatoma cell line. Journal of Virology. 1984;51(3):776-787.