Classical Xenopus laevis progesterone receptor associates to the plasma membrane through its ligand-binding domain

Silvana Martinez, Pamela Pastén, Karina Suarez, Andrea García, Francisco Nualart, Martín Montecino, María Victoria Hinrichs, Juan Olate

Resultado de la investigación: Article

14 Citas (Scopus)

Resumen

During the last decade, considerable evidence is accumulating that supports the view that the classic progesterone receptor (xPR-1) is mediating Xenopus laevis oocyte maturation through a non-genomic mechanism. Overexpression and depletion of oocyte xPR-1 have been shown to accelerate and to block progesterone-induced oocyte maturation, respectively. In addition, rapid inhibition of plasma membrane adenylyl cyclase (AC) by the steroid hormone, supports the idea that xPR-1 should be localized at the oocyte plasma membrane. To test this hypothesis, we transiently transfected xPR-1 cDNA into Cos-7 cells and analyzed its subcellular distribution. Through Western blot and immunofluorescence analysis, we were able to detect xPR-1 associated to the plasma membrane of transfected Cos-7 cells. Additionally, using Progesterone-BSA-FITC, we identified specific progesterone-binding sites at the cell surface of xPR-1 expressing cells. Finally, we found that the receptor ligand-binding domain displayed membrane localization, in contrast to the N-terminal domain, which expressed in similar levels, remained cytosolic. Overall, these results indicate that a fraction of xPR-1 expressed in Cos-7 cells, associates to the plasma membrane through its LBD.

Idioma originalEnglish
Páginas (desde-hasta)560-567
Número de páginas8
PublicaciónJournal of Cellular Physiology
Volumen211
N.º2
DOI
EstadoPublished - may 2007

Huella dactilar

Xenopus laevis
Progesterone Receptors
Cell membranes
Cell Membrane
Oocytes
Ligands
Progesterone
Steroid hormones
Adenylyl Cyclases
Complementary DNA
Binding Sites
Fluorescent Antibody Technique
Membranes
Western Blotting
Steroids
Hormones

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Citar esto

Martinez, Silvana ; Pastén, Pamela ; Suarez, Karina ; García, Andrea ; Nualart, Francisco ; Montecino, Martín ; Hinrichs, María Victoria ; Olate, Juan. / Classical Xenopus laevis progesterone receptor associates to the plasma membrane through its ligand-binding domain. En: Journal of Cellular Physiology. 2007 ; Vol. 211, N.º 2. pp. 560-567.
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abstract = "During the last decade, considerable evidence is accumulating that supports the view that the classic progesterone receptor (xPR-1) is mediating Xenopus laevis oocyte maturation through a non-genomic mechanism. Overexpression and depletion of oocyte xPR-1 have been shown to accelerate and to block progesterone-induced oocyte maturation, respectively. In addition, rapid inhibition of plasma membrane adenylyl cyclase (AC) by the steroid hormone, supports the idea that xPR-1 should be localized at the oocyte plasma membrane. To test this hypothesis, we transiently transfected xPR-1 cDNA into Cos-7 cells and analyzed its subcellular distribution. Through Western blot and immunofluorescence analysis, we were able to detect xPR-1 associated to the plasma membrane of transfected Cos-7 cells. Additionally, using Progesterone-BSA-FITC, we identified specific progesterone-binding sites at the cell surface of xPR-1 expressing cells. Finally, we found that the receptor ligand-binding domain displayed membrane localization, in contrast to the N-terminal domain, which expressed in similar levels, remained cytosolic. Overall, these results indicate that a fraction of xPR-1 expressed in Cos-7 cells, associates to the plasma membrane through its LBD.",
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Classical Xenopus laevis progesterone receptor associates to the plasma membrane through its ligand-binding domain. / Martinez, Silvana; Pastén, Pamela; Suarez, Karina; García, Andrea; Nualart, Francisco; Montecino, Martín; Hinrichs, María Victoria; Olate, Juan.

En: Journal of Cellular Physiology, Vol. 211, N.º 2, 05.2007, p. 560-567.

Resultado de la investigación: Article

TY - JOUR

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AU - Martinez, Silvana

AU - Pastén, Pamela

AU - Suarez, Karina

AU - García, Andrea

AU - Nualart, Francisco

AU - Montecino, Martín

AU - Hinrichs, María Victoria

AU - Olate, Juan

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