Chronic toxicity study of neosaxitoxin in rats

Ramiro J. Zepeda, Manila Candiracci, Nicolas Lobos, Sebastian Lux, Hugo F. Miranda

Resultado de la investigación: Contribución a una revistaArtículo

6 Citas (Scopus)

Resumen

Neosaxitoxin (NeoSTX) is a specific reversible blocker of voltage gated sodium channels on excitable cells. In the last decade, it has been tested in a number of interesting clinical trials, however there is still little information available on mammalian toxicity. Rats were treated for 12 weeks with doses of 1, 3 or 6 μg/kg of subcutaneous NeoSTX. At weeks 12 and 17, animals were sacrificed and blood samples collected for hematological and biochemical analysis. Organs were harvested for weight determination and histopathological assessments. The lowest acute toxicity via the intraperitoneal (ip) route was (30.35 μg/kg) and there was no significant difference between intramuscular and subcutaneous routes (11.4 and 12.41 μg/kg). The NeoSTX adiministration did not produce lethality at week 12 and after five weeks of suspension. NeoSTX 6 μg/kg ip produced reductions (p < 0.05) in body weight and food intake, and increased blood level of total and direct bilirubin, GGT and SGOT at week 12; all of these were reversed in the recovery period. NeoSTX 1 and 3 μg/kg ip did not show significant changes with the control group. Histopathological presentations were normal in all groups. This study revealed that NeoSTX is safe in vivo, giving a reliable security margin for its use like a local anesthetic.

Idioma originalInglés
Páginas (desde-hasta)5055-5071
Número de páginas17
PublicaciónMarine Drugs
Volumen12
N.º9
DOI
EstadoPublicada - 1 sep 2014

Áreas temáticas de ASJC Scopus

  • Descubrimiento de medicamentos

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  • Citar esto

    Zepeda, R. J., Candiracci, M., Lobos, N., Lux, S., & Miranda, H. F. (2014). Chronic toxicity study of neosaxitoxin in rats. Marine Drugs, 12(9), 5055-5071. https://doi.org/10.3390/md12095055