Chromatin remodeling by SWI/SNF results in nucleosome mobilization to preferential positions in the rat osteocalcin gene promoter

José Gutiérrez, Roberto Paredes, Fernando Cruzat, David A. Hill, Andre J. Van Wijnen, Jane B. Lian, Gary S. Stein, Janet L. Stein, Anthony N. Imbalzano, Martin Montecino

Resultado de la investigación: Article

27 Citas (Scopus)

Resumen

Changes in local chromatin structure accompany transcriptional activation of eukaryotic genes. In vivo these changes in chromatin organization can be catalyzed by ATP-dependent chromatin-remodeling complexes, such as SWI/SNF. These complexes alter the tight wrapping of DNA in the nucleosomes and can facilitate the mobilization of the histone octamer to adjacent DNA segments, leaving promoter regulatory elements exposed for transcription factor binding. To gain understanding of how the activity of SWI/SNF complexes may be modulated by the different DNA sequences within a natural promoter, we have reconstituted nucleosomes containing promoter segments of the transcriptionally active cell type-specific osteocalcin (OC) gene and determined how they affect the directional movements of the nucleosomes. Our results indicate that SWI/SNF complexes induce octamer sliding to preferential positions in the OC promoter, leading to a nucleosomal organization that resembles that described in intact cells expressing the OC gene. Our studies demonstrate that the position of the histone octamer is primarily determined by sequences within the OC promoter that include or exclude nucleosomes. We propose that these sequences are critical components of the regulatory mechanisms that mediate expression of this tissue-specific gene.

Idioma originalEnglish
Páginas (desde-hasta)9445-9457
Número de páginas13
PublicaciónJournal of Biological Chemistry
Volumen282
N.º13
DOI
EstadoPublished - 30 mar 2007

Huella dactilar

Chromatin Assembly and Disassembly
Nucleosomes
Osteocalcin
Chromatin
Rats
Genes
Histones
DNA sequences
DNA
Transcriptional Activation
Transcription Factors
Adenosine Triphosphate
Chemical activation
Cells
Tissue

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Citar esto

Gutiérrez, José ; Paredes, Roberto ; Cruzat, Fernando ; Hill, David A. ; Van Wijnen, Andre J. ; Lian, Jane B. ; Stein, Gary S. ; Stein, Janet L. ; Imbalzano, Anthony N. ; Montecino, Martin. / Chromatin remodeling by SWI/SNF results in nucleosome mobilization to preferential positions in the rat osteocalcin gene promoter. En: Journal of Biological Chemistry. 2007 ; Vol. 282, N.º 13. pp. 9445-9457.
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title = "Chromatin remodeling by SWI/SNF results in nucleosome mobilization to preferential positions in the rat osteocalcin gene promoter",
abstract = "Changes in local chromatin structure accompany transcriptional activation of eukaryotic genes. In vivo these changes in chromatin organization can be catalyzed by ATP-dependent chromatin-remodeling complexes, such as SWI/SNF. These complexes alter the tight wrapping of DNA in the nucleosomes and can facilitate the mobilization of the histone octamer to adjacent DNA segments, leaving promoter regulatory elements exposed for transcription factor binding. To gain understanding of how the activity of SWI/SNF complexes may be modulated by the different DNA sequences within a natural promoter, we have reconstituted nucleosomes containing promoter segments of the transcriptionally active cell type-specific osteocalcin (OC) gene and determined how they affect the directional movements of the nucleosomes. Our results indicate that SWI/SNF complexes induce octamer sliding to preferential positions in the OC promoter, leading to a nucleosomal organization that resembles that described in intact cells expressing the OC gene. Our studies demonstrate that the position of the histone octamer is primarily determined by sequences within the OC promoter that include or exclude nucleosomes. We propose that these sequences are critical components of the regulatory mechanisms that mediate expression of this tissue-specific gene.",
author = "Jos{\'e} Guti{\'e}rrez and Roberto Paredes and Fernando Cruzat and Hill, {David A.} and {Van Wijnen}, {Andre J.} and Lian, {Jane B.} and Stein, {Gary S.} and Stein, {Janet L.} and Imbalzano, {Anthony N.} and Martin Montecino",
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Gutiérrez, J, Paredes, R, Cruzat, F, Hill, DA, Van Wijnen, AJ, Lian, JB, Stein, GS, Stein, JL, Imbalzano, AN & Montecino, M 2007, 'Chromatin remodeling by SWI/SNF results in nucleosome mobilization to preferential positions in the rat osteocalcin gene promoter', Journal of Biological Chemistry, vol. 282, n.º 13, pp. 9445-9457. https://doi.org/10.1074/jbc.M609847200

Chromatin remodeling by SWI/SNF results in nucleosome mobilization to preferential positions in the rat osteocalcin gene promoter. / Gutiérrez, José; Paredes, Roberto; Cruzat, Fernando; Hill, David A.; Van Wijnen, Andre J.; Lian, Jane B.; Stein, Gary S.; Stein, Janet L.; Imbalzano, Anthony N.; Montecino, Martin.

En: Journal of Biological Chemistry, Vol. 282, N.º 13, 30.03.2007, p. 9445-9457.

Resultado de la investigación: Article

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AU - Gutiérrez, José

AU - Paredes, Roberto

AU - Cruzat, Fernando

AU - Hill, David A.

AU - Van Wijnen, Andre J.

AU - Lian, Jane B.

AU - Stein, Gary S.

AU - Stein, Janet L.

AU - Imbalzano, Anthony N.

AU - Montecino, Martin

PY - 2007/3/30

Y1 - 2007/3/30

N2 - Changes in local chromatin structure accompany transcriptional activation of eukaryotic genes. In vivo these changes in chromatin organization can be catalyzed by ATP-dependent chromatin-remodeling complexes, such as SWI/SNF. These complexes alter the tight wrapping of DNA in the nucleosomes and can facilitate the mobilization of the histone octamer to adjacent DNA segments, leaving promoter regulatory elements exposed for transcription factor binding. To gain understanding of how the activity of SWI/SNF complexes may be modulated by the different DNA sequences within a natural promoter, we have reconstituted nucleosomes containing promoter segments of the transcriptionally active cell type-specific osteocalcin (OC) gene and determined how they affect the directional movements of the nucleosomes. Our results indicate that SWI/SNF complexes induce octamer sliding to preferential positions in the OC promoter, leading to a nucleosomal organization that resembles that described in intact cells expressing the OC gene. Our studies demonstrate that the position of the histone octamer is primarily determined by sequences within the OC promoter that include or exclude nucleosomes. We propose that these sequences are critical components of the regulatory mechanisms that mediate expression of this tissue-specific gene.

AB - Changes in local chromatin structure accompany transcriptional activation of eukaryotic genes. In vivo these changes in chromatin organization can be catalyzed by ATP-dependent chromatin-remodeling complexes, such as SWI/SNF. These complexes alter the tight wrapping of DNA in the nucleosomes and can facilitate the mobilization of the histone octamer to adjacent DNA segments, leaving promoter regulatory elements exposed for transcription factor binding. To gain understanding of how the activity of SWI/SNF complexes may be modulated by the different DNA sequences within a natural promoter, we have reconstituted nucleosomes containing promoter segments of the transcriptionally active cell type-specific osteocalcin (OC) gene and determined how they affect the directional movements of the nucleosomes. Our results indicate that SWI/SNF complexes induce octamer sliding to preferential positions in the OC promoter, leading to a nucleosomal organization that resembles that described in intact cells expressing the OC gene. Our studies demonstrate that the position of the histone octamer is primarily determined by sequences within the OC promoter that include or exclude nucleosomes. We propose that these sequences are critical components of the regulatory mechanisms that mediate expression of this tissue-specific gene.

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