Chemical study and anti-inflammatory, analgesic and antioxidant activities of the leaves of Aristotelia chilensis (Mol.) Stuntz, Elaeocarpaceae

Orlando Muñoz, Philippe Christen, Sylvian Cretton, Nadine Backhouse, Vanessa Torres, Olosmira Correa, Edda Costa, Hugo Miranda, Carla Delporte

Resultado de la investigación: Article

28 Citas (Scopus)

Resumen

Objectives Aristotelia chilensis leaves (Elaeocarpaceae) are used in Chilean folk medicine to treat pain and inflammation. A bioguided study was carried out on serial extracts (hexane, dichloromethane, methanol, aqueous extract (INFU) and a crude mixture of alkaloids (ALK-MIX). All extracts were evaluated for (1) topical administration against both arachidonic acid and 12-deoxyphorbol-13-decanoate (TPA)-induced inflammation in mice and (2) per-os administration against inflammation by λ-carrageenan-induced paw oedema in guinea-pigs and (3) topical analgesia in tail flick and formalin models and per-os writhing test in mice. Methods Greater anti-inflammatory effects were obtained against TPA with dichloromethane extract and methanol extract (63.9 and 66.0%, respectively). INFU showed the most potent effect (56.2%) against arachidonic acid. Greater effects were obtained in the writhing test with hexane and dichloromethane extracts (89.2% both). In the topical analgesia models, all the extracts and ALK-MIX were active with exception of the hexane extract in the formalin assay. In tail flick test, ALK-MIX and the methanol extract were the most active (58.2 and 55.2%, respectively). In relation to the tail formalin assay, the methanol extract (74.1%) was the most active. Concerning antioxidant activity, both INFU and the methanol extract were the most active either in the inhibition of xanthine oxidase (52.9 and 62.7%, respectively) or in the DPPH free radical scavenging activity (EC50 (concentration that produced 50% of activity) = 12.1 and 9.7 μg/ml, respectively). Key findings Aristoteline, aristone, serratoline and hobartinol were isolated from ALK-MIX. Ursolic acid, friedelin and quercetin 5,3′-dimethyl ether were present in the dichloromethane extract while quercetin 3-O-β-d-glucoside and kaempferol were present in the methanol extract. From INFU were isolated protopine, aristoteline and caffeic and ferulic acids. Conclusions The effects of A. chilensis are herein demonstrated, validating its use in traditional medicine. Protopine is reported for the first time in Elaeocarpaceae.

Idioma originalEnglish
Páginas (desde-hasta)849-859
Número de páginas11
PublicaciónJournal of Pharmacy and Pharmacology
Volumen63
N.º6
DOI
EstadoPublished - jun 2011

Huella dactilar

Elaeocarpaceae
Non-Steroidal Anti-Inflammatory Agents
Methanol
Antioxidants
Methylene Chloride
Hexanes
Formaldehyde
Tail
Quercetin
Traditional Medicine
Inflammation
Arachidonic Acid
Analgesia
Caffeic Acids
Topical Administration
Xanthine Oxidase
Carrageenan
Alkaloids
Free Radicals
Edema

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

Citar esto

Muñoz, Orlando ; Christen, Philippe ; Cretton, Sylvian ; Backhouse, Nadine ; Torres, Vanessa ; Correa, Olosmira ; Costa, Edda ; Miranda, Hugo ; Delporte, Carla. / Chemical study and anti-inflammatory, analgesic and antioxidant activities of the leaves of Aristotelia chilensis (Mol.) Stuntz, Elaeocarpaceae. En: Journal of Pharmacy and Pharmacology. 2011 ; Vol. 63, N.º 6. pp. 849-859.
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title = "Chemical study and anti-inflammatory, analgesic and antioxidant activities of the leaves of Aristotelia chilensis (Mol.) Stuntz, Elaeocarpaceae",
abstract = "Objectives Aristotelia chilensis leaves (Elaeocarpaceae) are used in Chilean folk medicine to treat pain and inflammation. A bioguided study was carried out on serial extracts (hexane, dichloromethane, methanol, aqueous extract (INFU) and a crude mixture of alkaloids (ALK-MIX). All extracts were evaluated for (1) topical administration against both arachidonic acid and 12-deoxyphorbol-13-decanoate (TPA)-induced inflammation in mice and (2) per-os administration against inflammation by λ-carrageenan-induced paw oedema in guinea-pigs and (3) topical analgesia in tail flick and formalin models and per-os writhing test in mice. Methods Greater anti-inflammatory effects were obtained against TPA with dichloromethane extract and methanol extract (63.9 and 66.0{\%}, respectively). INFU showed the most potent effect (56.2{\%}) against arachidonic acid. Greater effects were obtained in the writhing test with hexane and dichloromethane extracts (89.2{\%} both). In the topical analgesia models, all the extracts and ALK-MIX were active with exception of the hexane extract in the formalin assay. In tail flick test, ALK-MIX and the methanol extract were the most active (58.2 and 55.2{\%}, respectively). In relation to the tail formalin assay, the methanol extract (74.1{\%}) was the most active. Concerning antioxidant activity, both INFU and the methanol extract were the most active either in the inhibition of xanthine oxidase (52.9 and 62.7{\%}, respectively) or in the DPPH free radical scavenging activity (EC50 (concentration that produced 50{\%} of activity) = 12.1 and 9.7 μg/ml, respectively). Key findings Aristoteline, aristone, serratoline and hobartinol were isolated from ALK-MIX. Ursolic acid, friedelin and quercetin 5,3′-dimethyl ether were present in the dichloromethane extract while quercetin 3-O-β-d-glucoside and kaempferol were present in the methanol extract. From INFU were isolated protopine, aristoteline and caffeic and ferulic acids. Conclusions The effects of A. chilensis are herein demonstrated, validating its use in traditional medicine. Protopine is reported for the first time in Elaeocarpaceae.",
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Chemical study and anti-inflammatory, analgesic and antioxidant activities of the leaves of Aristotelia chilensis (Mol.) Stuntz, Elaeocarpaceae. / Muñoz, Orlando; Christen, Philippe; Cretton, Sylvian; Backhouse, Nadine; Torres, Vanessa; Correa, Olosmira; Costa, Edda; Miranda, Hugo; Delporte, Carla.

En: Journal of Pharmacy and Pharmacology, Vol. 63, N.º 6, 06.2011, p. 849-859.

Resultado de la investigación: Article

TY - JOUR

T1 - Chemical study and anti-inflammatory, analgesic and antioxidant activities of the leaves of Aristotelia chilensis (Mol.) Stuntz, Elaeocarpaceae

AU - Muñoz, Orlando

AU - Christen, Philippe

AU - Cretton, Sylvian

AU - Backhouse, Nadine

AU - Torres, Vanessa

AU - Correa, Olosmira

AU - Costa, Edda

AU - Miranda, Hugo

AU - Delporte, Carla

PY - 2011/6

Y1 - 2011/6

N2 - Objectives Aristotelia chilensis leaves (Elaeocarpaceae) are used in Chilean folk medicine to treat pain and inflammation. A bioguided study was carried out on serial extracts (hexane, dichloromethane, methanol, aqueous extract (INFU) and a crude mixture of alkaloids (ALK-MIX). All extracts were evaluated for (1) topical administration against both arachidonic acid and 12-deoxyphorbol-13-decanoate (TPA)-induced inflammation in mice and (2) per-os administration against inflammation by λ-carrageenan-induced paw oedema in guinea-pigs and (3) topical analgesia in tail flick and formalin models and per-os writhing test in mice. Methods Greater anti-inflammatory effects were obtained against TPA with dichloromethane extract and methanol extract (63.9 and 66.0%, respectively). INFU showed the most potent effect (56.2%) against arachidonic acid. Greater effects were obtained in the writhing test with hexane and dichloromethane extracts (89.2% both). In the topical analgesia models, all the extracts and ALK-MIX were active with exception of the hexane extract in the formalin assay. In tail flick test, ALK-MIX and the methanol extract were the most active (58.2 and 55.2%, respectively). In relation to the tail formalin assay, the methanol extract (74.1%) was the most active. Concerning antioxidant activity, both INFU and the methanol extract were the most active either in the inhibition of xanthine oxidase (52.9 and 62.7%, respectively) or in the DPPH free radical scavenging activity (EC50 (concentration that produced 50% of activity) = 12.1 and 9.7 μg/ml, respectively). Key findings Aristoteline, aristone, serratoline and hobartinol were isolated from ALK-MIX. Ursolic acid, friedelin and quercetin 5,3′-dimethyl ether were present in the dichloromethane extract while quercetin 3-O-β-d-glucoside and kaempferol were present in the methanol extract. From INFU were isolated protopine, aristoteline and caffeic and ferulic acids. Conclusions The effects of A. chilensis are herein demonstrated, validating its use in traditional medicine. Protopine is reported for the first time in Elaeocarpaceae.

AB - Objectives Aristotelia chilensis leaves (Elaeocarpaceae) are used in Chilean folk medicine to treat pain and inflammation. A bioguided study was carried out on serial extracts (hexane, dichloromethane, methanol, aqueous extract (INFU) and a crude mixture of alkaloids (ALK-MIX). All extracts were evaluated for (1) topical administration against both arachidonic acid and 12-deoxyphorbol-13-decanoate (TPA)-induced inflammation in mice and (2) per-os administration against inflammation by λ-carrageenan-induced paw oedema in guinea-pigs and (3) topical analgesia in tail flick and formalin models and per-os writhing test in mice. Methods Greater anti-inflammatory effects were obtained against TPA with dichloromethane extract and methanol extract (63.9 and 66.0%, respectively). INFU showed the most potent effect (56.2%) against arachidonic acid. Greater effects were obtained in the writhing test with hexane and dichloromethane extracts (89.2% both). In the topical analgesia models, all the extracts and ALK-MIX were active with exception of the hexane extract in the formalin assay. In tail flick test, ALK-MIX and the methanol extract were the most active (58.2 and 55.2%, respectively). In relation to the tail formalin assay, the methanol extract (74.1%) was the most active. Concerning antioxidant activity, both INFU and the methanol extract were the most active either in the inhibition of xanthine oxidase (52.9 and 62.7%, respectively) or in the DPPH free radical scavenging activity (EC50 (concentration that produced 50% of activity) = 12.1 and 9.7 μg/ml, respectively). Key findings Aristoteline, aristone, serratoline and hobartinol were isolated from ALK-MIX. Ursolic acid, friedelin and quercetin 5,3′-dimethyl ether were present in the dichloromethane extract while quercetin 3-O-β-d-glucoside and kaempferol were present in the methanol extract. From INFU were isolated protopine, aristoteline and caffeic and ferulic acids. Conclusions The effects of A. chilensis are herein demonstrated, validating its use in traditional medicine. Protopine is reported for the first time in Elaeocarpaceae.

KW - alkaloids

KW - Aristotelia chilensis

KW - flavonoids

KW - triterpenes

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U2 - 10.1111/j.2042-7158.2011.01280.x

DO - 10.1111/j.2042-7158.2011.01280.x

M3 - Article

C2 - 21585384

AN - SCOPUS:79956187996

VL - 63

SP - 849

EP - 859

JO - Journal of Pharmacy and Pharmacology

JF - Journal of Pharmacy and Pharmacology

SN - 0022-3573

IS - 6

ER -