Resumen
1. 1. Carp plasma DHL was isolated by a single chromatographic procedure using Affi-Gel Blue. Neither LDL nor VLDL were detected. 2. 2. Thus, HDL which comprises almost 40% of the total carp plasma proteins, also constitutes the major lipoprotein fraction in this fish, yielding two apolipoproteins: apo A-I and apo A-II with molecular weight of 29,500 and 12,000, respectively. These are present in a one to one molar ratio. 3. 3. Carp apo A-I and apo A-II lack cysteine and tryptophan. Although some similarities in the amino acid composition exist with respect to the same apoproteins from other organisms, antisera raised against carp apo-I cross-reacted only, and very slightly, with its rainbow trout counterpart. 4. 4. No reaction was detected with lamprey and the nothotenidae robalo fish. 5. 5. Four plasmatic apo A-I isoforms were identified. No differences were observed in carps acclimatized to winter and summer.
| Idioma original | English |
|---|---|
| Páginas (desde-hasta) | 787-793 |
| Número de páginas | 7 |
| Publicación | Comparative Biochemistry and Physiology -- Part B: Biochemistry and |
| Volumen | 92 |
| N.º | 4 |
| DOI | |
| Estado | Published - 1 ene 1989 |
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ASJC Scopus subject areas
- Physiology
- Biochemistry
- Molecular Biology
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Characterization of the major plasma apoliproteins of the high density lipoprotein in the carp (Cyprinus carpio). / Amthauer, Rodolfo; Villanueva, Julieta; Vera, Maria Ines; Concha, Margarita; Krauskopf, Manuel.
En: Comparative Biochemistry and Physiology -- Part B: Biochemistry and, Vol. 92, N.º 4, 01.01.1989, p. 787-793.Resultado de la investigación: Article
TY - JOUR
T1 - Characterization of the major plasma apoliproteins of the high density lipoprotein in the carp (Cyprinus carpio)
AU - Amthauer, Rodolfo
AU - Villanueva, Julieta
AU - Vera, Maria Ines
AU - Concha, Margarita
AU - Krauskopf, Manuel
PY - 1989/1/1
Y1 - 1989/1/1
N2 - 1. 1. Carp plasma DHL was isolated by a single chromatographic procedure using Affi-Gel Blue. Neither LDL nor VLDL were detected. 2. 2. Thus, HDL which comprises almost 40% of the total carp plasma proteins, also constitutes the major lipoprotein fraction in this fish, yielding two apolipoproteins: apo A-I and apo A-II with molecular weight of 29,500 and 12,000, respectively. These are present in a one to one molar ratio. 3. 3. Carp apo A-I and apo A-II lack cysteine and tryptophan. Although some similarities in the amino acid composition exist with respect to the same apoproteins from other organisms, antisera raised against carp apo-I cross-reacted only, and very slightly, with its rainbow trout counterpart. 4. 4. No reaction was detected with lamprey and the nothotenidae robalo fish. 5. 5. Four plasmatic apo A-I isoforms were identified. No differences were observed in carps acclimatized to winter and summer.
AB - 1. 1. Carp plasma DHL was isolated by a single chromatographic procedure using Affi-Gel Blue. Neither LDL nor VLDL were detected. 2. 2. Thus, HDL which comprises almost 40% of the total carp plasma proteins, also constitutes the major lipoprotein fraction in this fish, yielding two apolipoproteins: apo A-I and apo A-II with molecular weight of 29,500 and 12,000, respectively. These are present in a one to one molar ratio. 3. 3. Carp apo A-I and apo A-II lack cysteine and tryptophan. Although some similarities in the amino acid composition exist with respect to the same apoproteins from other organisms, antisera raised against carp apo-I cross-reacted only, and very slightly, with its rainbow trout counterpart. 4. 4. No reaction was detected with lamprey and the nothotenidae robalo fish. 5. 5. Four plasmatic apo A-I isoforms were identified. No differences were observed in carps acclimatized to winter and summer.
UR - http://www.scopus.com/inward/record.url?scp=0001155019&partnerID=8YFLogxK
U2 - 10.1016/0305-0491(89)90268-X
DO - 10.1016/0305-0491(89)90268-X
M3 - Article
VL - 92
SP - 787
EP - 793
JO - Comparative biochemistry and physiology. B, Comparative biochemistry
JF - Comparative biochemistry and physiology. B, Comparative biochemistry
SN - 1096-4959
IS - 4
ER -