Characterization of the major plasma apoliproteins of the high density lipoprotein in the carp (Cyprinus carpio)

Rodolfo Amthauer, Julieta Villanueva, Maria Ines Vera, Margarita Concha, Manuel Krauskopf

Resultado de la investigación: Article

39 Citas (Scopus)

Resumen

1. 1. Carp plasma DHL was isolated by a single chromatographic procedure using Affi-Gel Blue. Neither LDL nor VLDL were detected. 2. 2. Thus, HDL which comprises almost 40% of the total carp plasma proteins, also constitutes the major lipoprotein fraction in this fish, yielding two apolipoproteins: apo A-I and apo A-II with molecular weight of 29,500 and 12,000, respectively. These are present in a one to one molar ratio. 3. 3. Carp apo A-I and apo A-II lack cysteine and tryptophan. Although some similarities in the amino acid composition exist with respect to the same apoproteins from other organisms, antisera raised against carp apo-I cross-reacted only, and very slightly, with its rainbow trout counterpart. 4. 4. No reaction was detected with lamprey and the nothotenidae robalo fish. 5. 5. Four plasmatic apo A-I isoforms were identified. No differences were observed in carps acclimatized to winter and summer.

Idioma originalEnglish
Páginas (desde-hasta)787-793
Número de páginas7
PublicaciónComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volumen92
N.º4
DOI
EstadoPublished - 1 ene 1989

Huella dactilar

Carps
Apolipoprotein A-I
HDL Lipoproteins
Apolipoprotein A-II
Cibacron Blue F 3GA
Plasmas
Fish
Apoproteins
Apolipoproteins
Fishes
Tryptophan
Lipoproteins
Cysteine
Blood Proteins
Immune Sera
Protein Isoforms
Lampreys
Molecular weight
Oncorhynchus mykiss
Amino Acids

ASJC Scopus subject areas

  • Physiology
  • Biochemistry
  • Molecular Biology

Citar esto

Amthauer, Rodolfo ; Villanueva, Julieta ; Vera, Maria Ines ; Concha, Margarita ; Krauskopf, Manuel. / Characterization of the major plasma apoliproteins of the high density lipoprotein in the carp (Cyprinus carpio). En: Comparative Biochemistry and Physiology -- Part B: Biochemistry and. 1989 ; Vol. 92, N.º 4. pp. 787-793.
@article{9c723089a2974af597be42cfd4346ee9,
title = "Characterization of the major plasma apoliproteins of the high density lipoprotein in the carp (Cyprinus carpio)",
abstract = "1. 1. Carp plasma DHL was isolated by a single chromatographic procedure using Affi-Gel Blue. Neither LDL nor VLDL were detected. 2. 2. Thus, HDL which comprises almost 40{\%} of the total carp plasma proteins, also constitutes the major lipoprotein fraction in this fish, yielding two apolipoproteins: apo A-I and apo A-II with molecular weight of 29,500 and 12,000, respectively. These are present in a one to one molar ratio. 3. 3. Carp apo A-I and apo A-II lack cysteine and tryptophan. Although some similarities in the amino acid composition exist with respect to the same apoproteins from other organisms, antisera raised against carp apo-I cross-reacted only, and very slightly, with its rainbow trout counterpart. 4. 4. No reaction was detected with lamprey and the nothotenidae robalo fish. 5. 5. Four plasmatic apo A-I isoforms were identified. No differences were observed in carps acclimatized to winter and summer.",
author = "Rodolfo Amthauer and Julieta Villanueva and Vera, {Maria Ines} and Margarita Concha and Manuel Krauskopf",
year = "1989",
month = "1",
day = "1",
doi = "10.1016/0305-0491(89)90268-X",
language = "English",
volume = "92",
pages = "787--793",
journal = "Comparative biochemistry and physiology. B, Comparative biochemistry",
issn = "1096-4959",
publisher = "Elsevier Inc.",
number = "4",

}

Characterization of the major plasma apoliproteins of the high density lipoprotein in the carp (Cyprinus carpio). / Amthauer, Rodolfo; Villanueva, Julieta; Vera, Maria Ines; Concha, Margarita; Krauskopf, Manuel.

En: Comparative Biochemistry and Physiology -- Part B: Biochemistry and, Vol. 92, N.º 4, 01.01.1989, p. 787-793.

Resultado de la investigación: Article

TY - JOUR

T1 - Characterization of the major plasma apoliproteins of the high density lipoprotein in the carp (Cyprinus carpio)

AU - Amthauer, Rodolfo

AU - Villanueva, Julieta

AU - Vera, Maria Ines

AU - Concha, Margarita

AU - Krauskopf, Manuel

PY - 1989/1/1

Y1 - 1989/1/1

N2 - 1. 1. Carp plasma DHL was isolated by a single chromatographic procedure using Affi-Gel Blue. Neither LDL nor VLDL were detected. 2. 2. Thus, HDL which comprises almost 40% of the total carp plasma proteins, also constitutes the major lipoprotein fraction in this fish, yielding two apolipoproteins: apo A-I and apo A-II with molecular weight of 29,500 and 12,000, respectively. These are present in a one to one molar ratio. 3. 3. Carp apo A-I and apo A-II lack cysteine and tryptophan. Although some similarities in the amino acid composition exist with respect to the same apoproteins from other organisms, antisera raised against carp apo-I cross-reacted only, and very slightly, with its rainbow trout counterpart. 4. 4. No reaction was detected with lamprey and the nothotenidae robalo fish. 5. 5. Four plasmatic apo A-I isoforms were identified. No differences were observed in carps acclimatized to winter and summer.

AB - 1. 1. Carp plasma DHL was isolated by a single chromatographic procedure using Affi-Gel Blue. Neither LDL nor VLDL were detected. 2. 2. Thus, HDL which comprises almost 40% of the total carp plasma proteins, also constitutes the major lipoprotein fraction in this fish, yielding two apolipoproteins: apo A-I and apo A-II with molecular weight of 29,500 and 12,000, respectively. These are present in a one to one molar ratio. 3. 3. Carp apo A-I and apo A-II lack cysteine and tryptophan. Although some similarities in the amino acid composition exist with respect to the same apoproteins from other organisms, antisera raised against carp apo-I cross-reacted only, and very slightly, with its rainbow trout counterpart. 4. 4. No reaction was detected with lamprey and the nothotenidae robalo fish. 5. 5. Four plasmatic apo A-I isoforms were identified. No differences were observed in carps acclimatized to winter and summer.

UR - http://www.scopus.com/inward/record.url?scp=0001155019&partnerID=8YFLogxK

U2 - 10.1016/0305-0491(89)90268-X

DO - 10.1016/0305-0491(89)90268-X

M3 - Article

VL - 92

SP - 787

EP - 793

JO - Comparative biochemistry and physiology. B, Comparative biochemistry

JF - Comparative biochemistry and physiology. B, Comparative biochemistry

SN - 1096-4959

IS - 4

ER -