Characterization of the major plasma apoliproteins of the high density lipoprotein in the carp (Cyprinus carpio)

Rodolfo Amthauer, Julieta Villanueva, Maria Ines Vera, Margarita Concha, Manuel Krauskopf

Resultado de la investigación: Contribución a una revistaArtículo

39 Citas (Scopus)

Resumen

1. 1. Carp plasma DHL was isolated by a single chromatographic procedure using Affi-Gel Blue. Neither LDL nor VLDL were detected. 2. 2. Thus, HDL which comprises almost 40% of the total carp plasma proteins, also constitutes the major lipoprotein fraction in this fish, yielding two apolipoproteins: apo A-I and apo A-II with molecular weight of 29,500 and 12,000, respectively. These are present in a one to one molar ratio. 3. 3. Carp apo A-I and apo A-II lack cysteine and tryptophan. Although some similarities in the amino acid composition exist with respect to the same apoproteins from other organisms, antisera raised against carp apo-I cross-reacted only, and very slightly, with its rainbow trout counterpart. 4. 4. No reaction was detected with lamprey and the nothotenidae robalo fish. 5. 5. Four plasmatic apo A-I isoforms were identified. No differences were observed in carps acclimatized to winter and summer.

Idioma originalInglés
Páginas (desde-hasta)787-793
Número de páginas7
PublicaciónComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volumen92
N.º4
DOI
EstadoPublicada - 1 ene 1989

Áreas temáticas de ASJC Scopus

  • Fisiología
  • Bioquímica
  • Biología molecular

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