Characterization of the Arginine Decarboxylase Gene (ORF HP0422, speA) Involved in Acid Tolerance in Helicobacter pylori

Manuel Valenzuela, Aníbal Cáceres, Oscar Almarza, Denisse Bravo, Sarita Soto, Oscar Cerda, Héctor Toledo

Resultado de la investigación: Article

5 Citas (Scopus)

Resumen

Background: Helicobacter pylori is a motile microaerophilic bacterium that colonizes the human stomach. H. pylori infection triggers gastric diseases, such as gastritis, peptic ulcer and gastric cancer. Stomach represents a barrier for microorganism colonization, particularly because of its high hydrochloric acid concentration. The main mechanism developed by H. pylori to maintain intracellular pH homeostasis in this environment is the urease activity. However, urease negative strains can be also isolated from clinical samples, suggesting that H. pylori presents other components involved in acid resistance. Objective: Here, we present some evidence that the arginine decarboxylase gene (speA) in H. pylori could be involved in an acid adaptation mechanism similar to the one in Enterobacteriaceae, which is dependent on the presence of arginine. Methods: Indeed, speA mRNA and protein expression are acutely induced by acid stress. Results: Moreover, we showed that H. pylori uses arginine in an acid response mechanism required for its growth in acid conditions. Conclusion: Altogether, these results provide novel information regarding the H. pylori physiology and acid response mechanism.

Idioma originalEnglish
Páginas (desde-hasta)182-193
Número de páginas12
PublicaciónHelicobacter
Volumen19
N.º3
DOI
EstadoPublished - 2014

Huella dactilar

Helicobacter pylori
Pylorus
Open Reading Frames
Acids
Genes
Urease
Arginine
Stomach
Stomach Diseases
Hydrochloric Acid
Helicobacter Infections
Gastritis
Enterobacteriaceae
Peptic Ulcer
Stomach Neoplasms
arginine decarboxylase
Homeostasis
Bacteria
Messenger RNA
Growth

ASJC Scopus subject areas

  • Gastroenterology
  • Infectious Diseases
  • Medicine(all)

Citar esto

Valenzuela, M., Cáceres, A., Almarza, O., Bravo, D., Soto, S., Cerda, O., & Toledo, H. (2014). Characterization of the Arginine Decarboxylase Gene (ORF HP0422, speA) Involved in Acid Tolerance in Helicobacter pylori. Helicobacter, 19(3), 182-193. https://doi.org/10.1111/hel.12115
Valenzuela, Manuel ; Cáceres, Aníbal ; Almarza, Oscar ; Bravo, Denisse ; Soto, Sarita ; Cerda, Oscar ; Toledo, Héctor. / Characterization of the Arginine Decarboxylase Gene (ORF HP0422, speA) Involved in Acid Tolerance in Helicobacter pylori. En: Helicobacter. 2014 ; Vol. 19, N.º 3. pp. 182-193.
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title = "Characterization of the Arginine Decarboxylase Gene (ORF HP0422, speA) Involved in Acid Tolerance in Helicobacter pylori",
abstract = "Background: Helicobacter pylori is a motile microaerophilic bacterium that colonizes the human stomach. H. pylori infection triggers gastric diseases, such as gastritis, peptic ulcer and gastric cancer. Stomach represents a barrier for microorganism colonization, particularly because of its high hydrochloric acid concentration. The main mechanism developed by H. pylori to maintain intracellular pH homeostasis in this environment is the urease activity. However, urease negative strains can be also isolated from clinical samples, suggesting that H. pylori presents other components involved in acid resistance. Objective: Here, we present some evidence that the arginine decarboxylase gene (speA) in H. pylori could be involved in an acid adaptation mechanism similar to the one in Enterobacteriaceae, which is dependent on the presence of arginine. Methods: Indeed, speA mRNA and protein expression are acutely induced by acid stress. Results: Moreover, we showed that H. pylori uses arginine in an acid response mechanism required for its growth in acid conditions. Conclusion: Altogether, these results provide novel information regarding the H. pylori physiology and acid response mechanism.",
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Valenzuela, M, Cáceres, A, Almarza, O, Bravo, D, Soto, S, Cerda, O & Toledo, H 2014, 'Characterization of the Arginine Decarboxylase Gene (ORF HP0422, speA) Involved in Acid Tolerance in Helicobacter pylori', Helicobacter, vol. 19, n.º 3, pp. 182-193. https://doi.org/10.1111/hel.12115

Characterization of the Arginine Decarboxylase Gene (ORF HP0422, speA) Involved in Acid Tolerance in Helicobacter pylori. / Valenzuela, Manuel; Cáceres, Aníbal; Almarza, Oscar; Bravo, Denisse; Soto, Sarita; Cerda, Oscar; Toledo, Héctor.

En: Helicobacter, Vol. 19, N.º 3, 2014, p. 182-193.

Resultado de la investigación: Article

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T1 - Characterization of the Arginine Decarboxylase Gene (ORF HP0422, speA) Involved in Acid Tolerance in Helicobacter pylori

AU - Valenzuela, Manuel

AU - Cáceres, Aníbal

AU - Almarza, Oscar

AU - Bravo, Denisse

AU - Soto, Sarita

AU - Cerda, Oscar

AU - Toledo, Héctor

PY - 2014

Y1 - 2014

N2 - Background: Helicobacter pylori is a motile microaerophilic bacterium that colonizes the human stomach. H. pylori infection triggers gastric diseases, such as gastritis, peptic ulcer and gastric cancer. Stomach represents a barrier for microorganism colonization, particularly because of its high hydrochloric acid concentration. The main mechanism developed by H. pylori to maintain intracellular pH homeostasis in this environment is the urease activity. However, urease negative strains can be also isolated from clinical samples, suggesting that H. pylori presents other components involved in acid resistance. Objective: Here, we present some evidence that the arginine decarboxylase gene (speA) in H. pylori could be involved in an acid adaptation mechanism similar to the one in Enterobacteriaceae, which is dependent on the presence of arginine. Methods: Indeed, speA mRNA and protein expression are acutely induced by acid stress. Results: Moreover, we showed that H. pylori uses arginine in an acid response mechanism required for its growth in acid conditions. Conclusion: Altogether, these results provide novel information regarding the H. pylori physiology and acid response mechanism.

AB - Background: Helicobacter pylori is a motile microaerophilic bacterium that colonizes the human stomach. H. pylori infection triggers gastric diseases, such as gastritis, peptic ulcer and gastric cancer. Stomach represents a barrier for microorganism colonization, particularly because of its high hydrochloric acid concentration. The main mechanism developed by H. pylori to maintain intracellular pH homeostasis in this environment is the urease activity. However, urease negative strains can be also isolated from clinical samples, suggesting that H. pylori presents other components involved in acid resistance. Objective: Here, we present some evidence that the arginine decarboxylase gene (speA) in H. pylori could be involved in an acid adaptation mechanism similar to the one in Enterobacteriaceae, which is dependent on the presence of arginine. Methods: Indeed, speA mRNA and protein expression are acutely induced by acid stress. Results: Moreover, we showed that H. pylori uses arginine in an acid response mechanism required for its growth in acid conditions. Conclusion: Altogether, these results provide novel information regarding the H. pylori physiology and acid response mechanism.

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