Characterization of synthetic hydroxyproline-rich proteoglycans with arabinogalactan protein and extensin motifs in arabidopsis

José M. Estévez, Marcia J. Kieliszewski, Natalie Khitrov, Chris Somerville

Resultado de la investigación: Article

63 Citas (Scopus)

Resumen

A series of gene constructs encoding synthetic glycomodule peptides with N-terminal signal sequences and C-terminal green fluorescent proteins were expressed in transgenic Arabidopsis (Arabidopsis thaliana) under the control of the 35S promoter. The synthetic glycomodule peptides were composed of repetitive proline-containing motifs that have been previously found to be substrates for prolyl hydroxylases and subsequent O-glycosylation of the hydroxyproline residues. All of the constructs were secreted in aerial tissues, but not in roots. The amount of hydroxylation and glycosylation of the various constructs varied depending on the tissue. Also, accumulation of the proteins exhibited a high degree of cell-type specificity within various tissues due to posttranscriptional effects. The observations reveal a high level of complexity in the synthesis, secretion, and turnover of the glycoproteins.

Idioma originalEnglish
Páginas (desde-hasta)458-470
Número de páginas13
PublicaciónPlant Physiology
Volumen142
N.º2
DOI
EstadoPublished - 1 oct 2006
Publicado de forma externa

Huella dactilar

extensin
arabinogalactan proteins
Amino Acid Motifs
proteoglycans
hydroxyproline
Hydroxyproline
Proteoglycans
Arabidopsis
synthetic peptides
glycosylation
Glycosylation
procollagen-proline dioxygenase
Prolyl Hydroxylases
Peptides
hydroxylation
Hydroxylation
Protein Sorting Signals
Green Fluorescent Proteins
signal peptide
green fluorescent protein

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Citar esto

Estévez, José M. ; Kieliszewski, Marcia J. ; Khitrov, Natalie ; Somerville, Chris. / Characterization of synthetic hydroxyproline-rich proteoglycans with arabinogalactan protein and extensin motifs in arabidopsis. En: Plant Physiology. 2006 ; Vol. 142, N.º 2. pp. 458-470.
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Characterization of synthetic hydroxyproline-rich proteoglycans with arabinogalactan protein and extensin motifs in arabidopsis. / Estévez, José M.; Kieliszewski, Marcia J.; Khitrov, Natalie; Somerville, Chris.

En: Plant Physiology, Vol. 142, N.º 2, 01.10.2006, p. 458-470.

Resultado de la investigación: Article

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AB - A series of gene constructs encoding synthetic glycomodule peptides with N-terminal signal sequences and C-terminal green fluorescent proteins were expressed in transgenic Arabidopsis (Arabidopsis thaliana) under the control of the 35S promoter. The synthetic glycomodule peptides were composed of repetitive proline-containing motifs that have been previously found to be substrates for prolyl hydroxylases and subsequent O-glycosylation of the hydroxyproline residues. All of the constructs were secreted in aerial tissues, but not in roots. The amount of hydroxylation and glycosylation of the various constructs varied depending on the tissue. Also, accumulation of the proteins exhibited a high degree of cell-type specificity within various tissues due to posttranscriptional effects. The observations reveal a high level of complexity in the synthesis, secretion, and turnover of the glycoproteins.

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