Characterization of cross-reactive and serotype-specific epitopes on the nucleocapsid proteins of hantaviruses

Nicole D. Tischler, Mario Rosemblatt, Pablo D T Valenzuela

Resultado de la investigación: Article

36 Citas (Scopus)

Resumen

The hantavirus nucleocapsid (N) protein fulfills several key roles in virus replication and assembly and is the major antigen in humoral immune responses in humans and mice. Here we report on epitopes involved in serotype-specific and cross-reactive recognition of the N proteins of hantaviruses using monoclonal antibodies (mAbs) against the N proteins of Andes virus (ANDV) and Sin Nombre virus (SNV). The mAbs define at least twelve different epitopic patterns which span eight sequences, including amino acids 17-59, 66-78, 79-91, 157-169, 222-234, 244-263, 274-286 and 326-338 on the SNV and ANDV N proteins. Studies on the cross-reactivity of these mAbs with different hantavirus N proteins indicated that epitopes located within amino acids 244-286 are related to serotype specificity. We analyzed further the location of epitopes with available three-dimensional structure information including the N-terminal coiled-coil and derived exposed and hidden residues of these epitopes. The generated recombinant N proteins and the characterized mAbs are functional tools being now available for hantavirus diagnostics and replication studies.

Idioma originalEnglish
Páginas (desde-hasta)1-9
Número de páginas9
PublicaciónVirus Research
Volumen135
N.º1
DOI
EstadoPublished - 1 jul 2008

Huella dactilar

Hantavirus
Nucleocapsid Proteins
Epitopes
Sin Nombre virus
Monoclonal Antibodies
Proteins
Virus Assembly
Humoral Immunity
Virus Replication
Recombinant Proteins
Serogroup
Amino Acid Sequence
Antigens
Amino Acids

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Virology

Citar esto

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Characterization of cross-reactive and serotype-specific epitopes on the nucleocapsid proteins of hantaviruses. / Tischler, Nicole D.; Rosemblatt, Mario; Valenzuela, Pablo D T.

En: Virus Research, Vol. 135, N.º 1, 01.07.2008, p. 1-9.

Resultado de la investigación: Article

TY - JOUR

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AU - Valenzuela, Pablo D T

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AB - The hantavirus nucleocapsid (N) protein fulfills several key roles in virus replication and assembly and is the major antigen in humoral immune responses in humans and mice. Here we report on epitopes involved in serotype-specific and cross-reactive recognition of the N proteins of hantaviruses using monoclonal antibodies (mAbs) against the N proteins of Andes virus (ANDV) and Sin Nombre virus (SNV). The mAbs define at least twelve different epitopic patterns which span eight sequences, including amino acids 17-59, 66-78, 79-91, 157-169, 222-234, 244-263, 274-286 and 326-338 on the SNV and ANDV N proteins. Studies on the cross-reactivity of these mAbs with different hantavirus N proteins indicated that epitopes located within amino acids 244-286 are related to serotype specificity. We analyzed further the location of epitopes with available three-dimensional structure information including the N-terminal coiled-coil and derived exposed and hidden residues of these epitopes. The generated recombinant N proteins and the characterized mAbs are functional tools being now available for hantavirus diagnostics and replication studies.

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