Characterization of cell wall polysaccharides of the coencocytic green seaweed bryopsis plumosa (bryopsidaceae, chlorophyta) from the argentine coast

Marina Ciancia, Josefina Alberghina, Paula Ximena Arata, Hugo Benavides, Frederik Leliaert, Heroen Verbruggen, Jose Manuel Estevez

Resultado de la investigación: Article

23 Citas (Scopus)

Resumen

Bryopsis sp. from a restricted area of the rocky shore of Mar del Plata (Argentina) on the Atlantic coast was identified as Bryopsis plumosa (Hudson) C. Agardh (Bryopsidales, Chlorophyta) based on morphological characters and rbcL and tufA DNA barcodes. To analyze the cell wall polysaccharides of this seaweed, the major room temperature (B1) and 90°C (X1) water extracts were studied. By linkage analysis and NMR spectroscopy, the structure of a sulfated galactan was determined, and putative sulfated rhamnan structures and furanosidic nonsulfated arabinan structures were also found. By anion exchange chromatography of X1, a fraction (F4), comprising a sulfated galactan as major structure was isolated. Structural analysis showed a linear backbone constituted of 3-linked β-d-galactose units, partially sulfated on C-6 and partially substituted with pyruvic acid forming an acetal linked to O-4 and O-6. This galactan has common structural features with those of green seaweeds of the genus Codium (Bryopsidales, Chlorophyta), but some important differences were also found. This is the first report about the structure of the water-soluble polysaccharides biosynthesized by seaweeds of the genus Bryopsis. These sulfated galactans and rhamnans were in situ localized mostly in two layers, one close to the plasma membrane and the other close to the apoplast, leaving a middle amorphous, unstained cell wall zone. In addition, fibrillar polysaccharides, comprising (1→3)-β-d-xylans and cellulose, were obtained by treatment of the residue from the water extractions with an LiCl/DMSO solution at high temperature. These polymers were also localized in a bilayer arrangement.

Idioma originalEnglish
Páginas (desde-hasta)326-335
Número de páginas10
PublicaciónJournal of Phycology
Volumen48
N.º2
DOI
EstadoPublished - 1 abr 2012
Publicado de forma externa

Huella dactilar

Bryopsidaceae
Bryopsis
galactans
seaweed
polysaccharide
Chlorophyta
macroalgae
polysaccharides
Bryopsidales
cell walls
coasts
coast
tufa
rocky shore
structural analysis
Codium
pyruvic acid
chromatography
nuclear magnetic resonance
cellulose

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science

Citar esto

Ciancia, Marina ; Alberghina, Josefina ; Arata, Paula Ximena ; Benavides, Hugo ; Leliaert, Frederik ; Verbruggen, Heroen ; Estevez, Jose Manuel. / Characterization of cell wall polysaccharides of the coencocytic green seaweed bryopsis plumosa (bryopsidaceae, chlorophyta) from the argentine coast. En: Journal of Phycology. 2012 ; Vol. 48, N.º 2. pp. 326-335.
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title = "Characterization of cell wall polysaccharides of the coencocytic green seaweed bryopsis plumosa (bryopsidaceae, chlorophyta) from the argentine coast",
abstract = "Bryopsis sp. from a restricted area of the rocky shore of Mar del Plata (Argentina) on the Atlantic coast was identified as Bryopsis plumosa (Hudson) C. Agardh (Bryopsidales, Chlorophyta) based on morphological characters and rbcL and tufA DNA barcodes. To analyze the cell wall polysaccharides of this seaweed, the major room temperature (B1) and 90°C (X1) water extracts were studied. By linkage analysis and NMR spectroscopy, the structure of a sulfated galactan was determined, and putative sulfated rhamnan structures and furanosidic nonsulfated arabinan structures were also found. By anion exchange chromatography of X1, a fraction (F4), comprising a sulfated galactan as major structure was isolated. Structural analysis showed a linear backbone constituted of 3-linked β-d-galactose units, partially sulfated on C-6 and partially substituted with pyruvic acid forming an acetal linked to O-4 and O-6. This galactan has common structural features with those of green seaweeds of the genus Codium (Bryopsidales, Chlorophyta), but some important differences were also found. This is the first report about the structure of the water-soluble polysaccharides biosynthesized by seaweeds of the genus Bryopsis. These sulfated galactans and rhamnans were in situ localized mostly in two layers, one close to the plasma membrane and the other close to the apoplast, leaving a middle amorphous, unstained cell wall zone. In addition, fibrillar polysaccharides, comprising (1→3)-β-d-xylans and cellulose, were obtained by treatment of the residue from the water extractions with an LiCl/DMSO solution at high temperature. These polymers were also localized in a bilayer arrangement.",
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Characterization of cell wall polysaccharides of the coencocytic green seaweed bryopsis plumosa (bryopsidaceae, chlorophyta) from the argentine coast. / Ciancia, Marina; Alberghina, Josefina; Arata, Paula Ximena; Benavides, Hugo; Leliaert, Frederik; Verbruggen, Heroen; Estevez, Jose Manuel.

En: Journal of Phycology, Vol. 48, N.º 2, 01.04.2012, p. 326-335.

Resultado de la investigación: Article

TY - JOUR

T1 - Characterization of cell wall polysaccharides of the coencocytic green seaweed bryopsis plumosa (bryopsidaceae, chlorophyta) from the argentine coast

AU - Ciancia, Marina

AU - Alberghina, Josefina

AU - Arata, Paula Ximena

AU - Benavides, Hugo

AU - Leliaert, Frederik

AU - Verbruggen, Heroen

AU - Estevez, Jose Manuel

PY - 2012/4/1

Y1 - 2012/4/1

N2 - Bryopsis sp. from a restricted area of the rocky shore of Mar del Plata (Argentina) on the Atlantic coast was identified as Bryopsis plumosa (Hudson) C. Agardh (Bryopsidales, Chlorophyta) based on morphological characters and rbcL and tufA DNA barcodes. To analyze the cell wall polysaccharides of this seaweed, the major room temperature (B1) and 90°C (X1) water extracts were studied. By linkage analysis and NMR spectroscopy, the structure of a sulfated galactan was determined, and putative sulfated rhamnan structures and furanosidic nonsulfated arabinan structures were also found. By anion exchange chromatography of X1, a fraction (F4), comprising a sulfated galactan as major structure was isolated. Structural analysis showed a linear backbone constituted of 3-linked β-d-galactose units, partially sulfated on C-6 and partially substituted with pyruvic acid forming an acetal linked to O-4 and O-6. This galactan has common structural features with those of green seaweeds of the genus Codium (Bryopsidales, Chlorophyta), but some important differences were also found. This is the first report about the structure of the water-soluble polysaccharides biosynthesized by seaweeds of the genus Bryopsis. These sulfated galactans and rhamnans were in situ localized mostly in two layers, one close to the plasma membrane and the other close to the apoplast, leaving a middle amorphous, unstained cell wall zone. In addition, fibrillar polysaccharides, comprising (1→3)-β-d-xylans and cellulose, were obtained by treatment of the residue from the water extractions with an LiCl/DMSO solution at high temperature. These polymers were also localized in a bilayer arrangement.

AB - Bryopsis sp. from a restricted area of the rocky shore of Mar del Plata (Argentina) on the Atlantic coast was identified as Bryopsis plumosa (Hudson) C. Agardh (Bryopsidales, Chlorophyta) based on morphological characters and rbcL and tufA DNA barcodes. To analyze the cell wall polysaccharides of this seaweed, the major room temperature (B1) and 90°C (X1) water extracts were studied. By linkage analysis and NMR spectroscopy, the structure of a sulfated galactan was determined, and putative sulfated rhamnan structures and furanosidic nonsulfated arabinan structures were also found. By anion exchange chromatography of X1, a fraction (F4), comprising a sulfated galactan as major structure was isolated. Structural analysis showed a linear backbone constituted of 3-linked β-d-galactose units, partially sulfated on C-6 and partially substituted with pyruvic acid forming an acetal linked to O-4 and O-6. This galactan has common structural features with those of green seaweeds of the genus Codium (Bryopsidales, Chlorophyta), but some important differences were also found. This is the first report about the structure of the water-soluble polysaccharides biosynthesized by seaweeds of the genus Bryopsis. These sulfated galactans and rhamnans were in situ localized mostly in two layers, one close to the plasma membrane and the other close to the apoplast, leaving a middle amorphous, unstained cell wall zone. In addition, fibrillar polysaccharides, comprising (1→3)-β-d-xylans and cellulose, were obtained by treatment of the residue from the water extractions with an LiCl/DMSO solution at high temperature. These polymers were also localized in a bilayer arrangement.

KW - Bryopsis plumose

KW - Cell wall

KW - Green seaweed

KW - Pyruvylated galactan sulfate

KW - Sulfated polysaccharides

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DO - 10.1111/j.1529-8817.2012.01131.x

M3 - Article

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