Characterization of an α-L-arabinofuranosidase gene (abf1) from Penicillium purpurogenum and its expression

Marcela Carvallo, Pablo De Ioannes, Claudio Navarro, Renato Chavez, Alessandra Peirano, Paulina Bull, Jaime Eyzaguirre

Resultado de la investigación: Article

17 Citas (Scopus)

Resumen

An α-L-arabinofuranosidase gene (abf1) from Penicillium purpurogenum was identified and sequenced. abf1 has an open reading frame of 1518 bp, does not contain introns and codes for a protein of 506 amino acids. The deduced mature protein has a molecular mass of 49.6 KDa, and its sequence is homologous to arabinofuranosidases of glycosyl hydrolase family 54. Southern blots suggest that abf1 is a single copy gene. Putative sequences for the binding of the transcriptional regulators XlnR, CreA, PacC, AlcR and Area are present in the promoter. Northern-blot analysis shows that abf1 is expressed at neutral but not at alkaline or acidic pH values. The presence of binding sites for regulatory elements in the promoter region has been compared to the genes of other fungal enzymes belonging to the same family. This is the first characterization of an abf gene from a Penicillium species.

Idioma originalEnglish
Páginas (desde-hasta)388-394
Número de páginas7
PublicaciónMycological Research
Volumen107
N.º4
DOI
EstadoPublished - 1 abr 2003

Huella dactilar

Talaromyces purpurogenus
alpha-N-arabinofuranosidase
Penicillium
gene
Fungal Genes
Genes
genes
promoter regions
Hydrolases
Sequence Homology
Southern Blotting
Genetic Promoter Regions
Northern Blotting
Introns
Open Reading Frames
protein
glycosidases
Proteins
Binding Sites
Northern blotting

ASJC Scopus subject areas

  • Biotechnology
  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Plant Science

Citar esto

Carvallo, Marcela ; De Ioannes, Pablo ; Navarro, Claudio ; Chavez, Renato ; Peirano, Alessandra ; Bull, Paulina ; Eyzaguirre, Jaime. / Characterization of an α-L-arabinofuranosidase gene (abf1) from Penicillium purpurogenum and its expression. En: Mycological Research. 2003 ; Vol. 107, N.º 4. pp. 388-394.
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abstract = "An α-L-arabinofuranosidase gene (abf1) from Penicillium purpurogenum was identified and sequenced. abf1 has an open reading frame of 1518 bp, does not contain introns and codes for a protein of 506 amino acids. The deduced mature protein has a molecular mass of 49.6 KDa, and its sequence is homologous to arabinofuranosidases of glycosyl hydrolase family 54. Southern blots suggest that abf1 is a single copy gene. Putative sequences for the binding of the transcriptional regulators XlnR, CreA, PacC, AlcR and Area are present in the promoter. Northern-blot analysis shows that abf1 is expressed at neutral but not at alkaline or acidic pH values. The presence of binding sites for regulatory elements in the promoter region has been compared to the genes of other fungal enzymes belonging to the same family. This is the first characterization of an abf gene from a Penicillium species.",
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Characterization of an α-L-arabinofuranosidase gene (abf1) from Penicillium purpurogenum and its expression. / Carvallo, Marcela; De Ioannes, Pablo; Navarro, Claudio; Chavez, Renato; Peirano, Alessandra; Bull, Paulina; Eyzaguirre, Jaime.

En: Mycological Research, Vol. 107, N.º 4, 01.04.2003, p. 388-394.

Resultado de la investigación: Article

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AU - Peirano, Alessandra

AU - Bull, Paulina

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