Characterization of an α-L-arabinofuranosidase gene (abf1) from Penicillium purpurogenum and its expression

Marcela Carvallo, Pablo De Ioannes, Claudio Navarro, Renato Chavez, Alessandra Peirano, Paulina Bull, Jaime Eyzaguirre

Resultado de la investigación: Contribución a una revistaArtículo

17 Citas (Scopus)

Resumen

An α-L-arabinofuranosidase gene (abf1) from Penicillium purpurogenum was identified and sequenced. abf1 has an open reading frame of 1518 bp, does not contain introns and codes for a protein of 506 amino acids. The deduced mature protein has a molecular mass of 49.6 KDa, and its sequence is homologous to arabinofuranosidases of glycosyl hydrolase family 54. Southern blots suggest that abf1 is a single copy gene. Putative sequences for the binding of the transcriptional regulators XlnR, CreA, PacC, AlcR and Area are present in the promoter. Northern-blot analysis shows that abf1 is expressed at neutral but not at alkaline or acidic pH values. The presence of binding sites for regulatory elements in the promoter region has been compared to the genes of other fungal enzymes belonging to the same family. This is the first characterization of an abf gene from a Penicillium species.

Idioma originalInglés
Páginas (desde-hasta)388-394
Número de páginas7
PublicaciónMycological Research
Volumen107
N.º4
DOI
EstadoPublicada - 1 abr 2003

Áreas temáticas de ASJC Scopus

  • Biotecnología
  • Ecología, evolución, comportamiento y sistemática
  • Genética
  • Botánica

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