Characterization of 14-3-3 isoforms expressed in the Echinococcus granulosus pathogenic larval stage

Aline Teichmann, Daiani M. Vargas, Karina M. Monteiro, Bruna V. Meneghetti, Cristine S. Dutra, Rodolfo Paredes, Norbel Galanti, Arnaldo Zaha, Henrique B. Ferreira

Resultado de la investigación: Article

11 Citas (Scopus)

Resumen

The 14-3-3 protein family of eukaryotic regulators was studied in Echinococcus granulosus, the causative agent of cystic hydatid disease. These proteins mediate important cellular processes in eukaryotes and are expected to play important roles in parasite biology. Six isoforms of E. granulosus 14-3-3 genes and proteins (Eg14-3-3.1-6) were analyzed, and their phylogenetic relationships were established with bona fide 14-3-3 orthologous proteins from eukaryotic species. Eg14-3-3 isoforms with previous evidence of expression (Eg14-3-3.1-4) in E. granulosus pathogenic larval stage (metacestode) were cloned, and recombinant proteins were used for functional studies. These protein isoforms were detected in different components of E. granulosus metacestode, including interface components with the host. The roles that are played by Eg14-3-3 proteins in parasite biology were inferred from the repertoires of interacting proteins with each isoform, as assessed by gel overlay, cross-linking, and affinity chromatography assays. A total of 95 Eg14-3-3 protein ligands were identified by mass spectrometry. Eg14-3-3 isoforms have shared partners (44 proteins), indicating some overlapping functions; however, they also bind exclusive partners (51 proteins), suggesting Eg14-3-3 functional specialization. These ligand repertoires indicate the involvement of Eg14-3-3 proteins in multiple biochemical pathways in the E. granulosus metacestode and note some degree of isoform specialization.

Idioma originalEnglish
Páginas (desde-hasta)1700-1715
Número de páginas16
PublicaciónJournal of Proteome Research
Volumen14
N.º4
DOI
EstadoPublished - 1 ene 2015

Huella dactilar

Echinococcus granulosus
Protein Isoforms
14-3-3 Proteins
Proteins
Parasites
Ligands
Affinity chromatography
Eukaryota
Affinity Chromatography
Recombinant Proteins
Mass spectrometry
Assays
Mass Spectrometry
Gels

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Citar esto

Teichmann, A., Vargas, D. M., Monteiro, K. M., Meneghetti, B. V., Dutra, C. S., Paredes, R., ... Ferreira, H. B. (2015). Characterization of 14-3-3 isoforms expressed in the Echinococcus granulosus pathogenic larval stage. Journal of Proteome Research, 14(4), 1700-1715. https://doi.org/10.1021/pr5010136
Teichmann, Aline ; Vargas, Daiani M. ; Monteiro, Karina M. ; Meneghetti, Bruna V. ; Dutra, Cristine S. ; Paredes, Rodolfo ; Galanti, Norbel ; Zaha, Arnaldo ; Ferreira, Henrique B. / Characterization of 14-3-3 isoforms expressed in the Echinococcus granulosus pathogenic larval stage. En: Journal of Proteome Research. 2015 ; Vol. 14, N.º 4. pp. 1700-1715.
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abstract = "The 14-3-3 protein family of eukaryotic regulators was studied in Echinococcus granulosus, the causative agent of cystic hydatid disease. These proteins mediate important cellular processes in eukaryotes and are expected to play important roles in parasite biology. Six isoforms of E. granulosus 14-3-3 genes and proteins (Eg14-3-3.1-6) were analyzed, and their phylogenetic relationships were established with bona fide 14-3-3 orthologous proteins from eukaryotic species. Eg14-3-3 isoforms with previous evidence of expression (Eg14-3-3.1-4) in E. granulosus pathogenic larval stage (metacestode) were cloned, and recombinant proteins were used for functional studies. These protein isoforms were detected in different components of E. granulosus metacestode, including interface components with the host. The roles that are played by Eg14-3-3 proteins in parasite biology were inferred from the repertoires of interacting proteins with each isoform, as assessed by gel overlay, cross-linking, and affinity chromatography assays. A total of 95 Eg14-3-3 protein ligands were identified by mass spectrometry. Eg14-3-3 isoforms have shared partners (44 proteins), indicating some overlapping functions; however, they also bind exclusive partners (51 proteins), suggesting Eg14-3-3 functional specialization. These ligand repertoires indicate the involvement of Eg14-3-3 proteins in multiple biochemical pathways in the E. granulosus metacestode and note some degree of isoform specialization.",
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Teichmann, A, Vargas, DM, Monteiro, KM, Meneghetti, BV, Dutra, CS, Paredes, R, Galanti, N, Zaha, A & Ferreira, HB 2015, 'Characterization of 14-3-3 isoforms expressed in the Echinococcus granulosus pathogenic larval stage', Journal of Proteome Research, vol. 14, n.º 4, pp. 1700-1715. https://doi.org/10.1021/pr5010136

Characterization of 14-3-3 isoforms expressed in the Echinococcus granulosus pathogenic larval stage. / Teichmann, Aline; Vargas, Daiani M.; Monteiro, Karina M.; Meneghetti, Bruna V.; Dutra, Cristine S.; Paredes, Rodolfo; Galanti, Norbel; Zaha, Arnaldo; Ferreira, Henrique B.

En: Journal of Proteome Research, Vol. 14, N.º 4, 01.01.2015, p. 1700-1715.

Resultado de la investigación: Article

TY - JOUR

T1 - Characterization of 14-3-3 isoforms expressed in the Echinococcus granulosus pathogenic larval stage

AU - Teichmann, Aline

AU - Vargas, Daiani M.

AU - Monteiro, Karina M.

AU - Meneghetti, Bruna V.

AU - Dutra, Cristine S.

AU - Paredes, Rodolfo

AU - Galanti, Norbel

AU - Zaha, Arnaldo

AU - Ferreira, Henrique B.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - The 14-3-3 protein family of eukaryotic regulators was studied in Echinococcus granulosus, the causative agent of cystic hydatid disease. These proteins mediate important cellular processes in eukaryotes and are expected to play important roles in parasite biology. Six isoforms of E. granulosus 14-3-3 genes and proteins (Eg14-3-3.1-6) were analyzed, and their phylogenetic relationships were established with bona fide 14-3-3 orthologous proteins from eukaryotic species. Eg14-3-3 isoforms with previous evidence of expression (Eg14-3-3.1-4) in E. granulosus pathogenic larval stage (metacestode) were cloned, and recombinant proteins were used for functional studies. These protein isoforms were detected in different components of E. granulosus metacestode, including interface components with the host. The roles that are played by Eg14-3-3 proteins in parasite biology were inferred from the repertoires of interacting proteins with each isoform, as assessed by gel overlay, cross-linking, and affinity chromatography assays. A total of 95 Eg14-3-3 protein ligands were identified by mass spectrometry. Eg14-3-3 isoforms have shared partners (44 proteins), indicating some overlapping functions; however, they also bind exclusive partners (51 proteins), suggesting Eg14-3-3 functional specialization. These ligand repertoires indicate the involvement of Eg14-3-3 proteins in multiple biochemical pathways in the E. granulosus metacestode and note some degree of isoform specialization.

AB - The 14-3-3 protein family of eukaryotic regulators was studied in Echinococcus granulosus, the causative agent of cystic hydatid disease. These proteins mediate important cellular processes in eukaryotes and are expected to play important roles in parasite biology. Six isoforms of E. granulosus 14-3-3 genes and proteins (Eg14-3-3.1-6) were analyzed, and their phylogenetic relationships were established with bona fide 14-3-3 orthologous proteins from eukaryotic species. Eg14-3-3 isoforms with previous evidence of expression (Eg14-3-3.1-4) in E. granulosus pathogenic larval stage (metacestode) were cloned, and recombinant proteins were used for functional studies. These protein isoforms were detected in different components of E. granulosus metacestode, including interface components with the host. The roles that are played by Eg14-3-3 proteins in parasite biology were inferred from the repertoires of interacting proteins with each isoform, as assessed by gel overlay, cross-linking, and affinity chromatography assays. A total of 95 Eg14-3-3 protein ligands were identified by mass spectrometry. Eg14-3-3 isoforms have shared partners (44 proteins), indicating some overlapping functions; however, they also bind exclusive partners (51 proteins), suggesting Eg14-3-3 functional specialization. These ligand repertoires indicate the involvement of Eg14-3-3 proteins in multiple biochemical pathways in the E. granulosus metacestode and note some degree of isoform specialization.

KW - 14-3-3 proteins

KW - Echinococcus granulosus

KW - host-parasite interactions

KW - protein-protein interactions

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