Characterization and Salt Response in Recurrent Halotolerant Exiguobacterium sp SH31 Isolated From Sediments of Salar de Huasco, Chilean Altiplano

Francisco Remonsellez, Juan Castro-Severyn, Coral Pardo-Este, Pablo Aguilar, Jonathan Fortt, Cesar Salinas, Sergio Barahona, Joice Leon, Barbara Fuentes, Carlos Areche, Klaudia L. Hernandez, Claudia P. Saavedra, Daniel Aguayo

Resultado de la investigación: Article

Resumen

Poly-extremophiles microorganisms have the capacity to inhabit hostile environments and can survive several adverse conditions that include as variations in temperature, pH, and salinity, high levels UV light and atmospheric pressure, and even the presence of toxic compounds and the formation of reactive oxygen species (ROS). A halotolerant Exiguobacterium strain was isolated from Salar de Huasco (Chilean Altiplano), a well-known shallow lake area with variable salinity levels, little human intervention, and extreme environmental conditions, which makes it ideal for the study of resistant mechanisms and the evolution of adaptations. This bacterial genus has not been extensively studied, although its cosmopolitan location indicates that it has high levels of plasticity and adaptive capacity. However, to date, there are no studies regarding the tolerance and resistance to salinity and osmotic pressure. We set out to characterize the Exiguobacterium sp. SH31 strain and describe its phenotypical and genotypical response to osmotic stress. In this context, as a first step to characterize the response to the SH31 strain to salinity and to establish the bases for a molecular study, we proposed to compare its response under three salt conditions (0, 25, and 50 g/l NaCl). Using different physiology, genomic, and transcriptomic approaches, we determined that the bacterium is able to grow properly in a NaCl concentration of up to 50 g/l; however, the best growth rate was observed at 25 g/l. Although the presence of flagella is not affected by salinity, motility was diminished at 25 g/l NaCl and abolished at 50 g/l. Biofilm formation was induced proportionally with increases in salinity, which was expected. These phenotypic results correlated with the expression of related genes: fliG and fliS Motility); opuBA and putP (transport); glnA, proC, gltA, and gbsA (compatible solutes); ywqC, bdlA, luxS y pgaC (biofilm and stress response); and therefore, we conclude that this strain effectively modifies gene expression and physiology in a differential manner when faced with different concentrations of NaCl and these modifications aid survival.

Idioma originalEnglish
Número de artículo2228
PublicaciónFrontiers in Microbiology
Volumen9
N.ºSEP
DOI
EstadoPublished - 20 sep 2018

Keywords

    ASJC Scopus subject areas

    • Microbiology
    • Microbiology (medical)

    Citar esto

    Remonsellez, Francisco ; Castro-Severyn, Juan ; Pardo-Este, Coral ; Aguilar, Pablo ; Fortt, Jonathan ; Salinas, Cesar ; Barahona, Sergio ; Leon, Joice ; Fuentes, Barbara ; Areche, Carlos ; Hernandez, Klaudia L. ; Saavedra, Claudia P. ; Aguayo, Daniel. / Characterization and Salt Response in Recurrent Halotolerant Exiguobacterium sp SH31 Isolated From Sediments of Salar de Huasco, Chilean Altiplano. En: Frontiers in Microbiology. 2018 ; Vol. 9, N.º SEP.
    @article{4d1930a2d6b44a9386c1d4804b007cdf,
    title = "Characterization and Salt Response in Recurrent Halotolerant Exiguobacterium sp SH31 Isolated From Sediments of Salar de Huasco, Chilean Altiplano",
    abstract = "Poly-extremophiles microorganisms have the capacity to inhabit hostile environments and can survive several adverse conditions that include as variations in temperature, pH, and salinity, high levels UV light and atmospheric pressure, and even the presence of toxic compounds and the formation of reactive oxygen species (ROS). A halotolerant Exiguobacterium strain was isolated from Salar de Huasco (Chilean Altiplano), a well-known shallow lake area with variable salinity levels, little human intervention, and extreme environmental conditions, which makes it ideal for the study of resistant mechanisms and the evolution of adaptations. This bacterial genus has not been extensively studied, although its cosmopolitan location indicates that it has high levels of plasticity and adaptive capacity. However, to date, there are no studies regarding the tolerance and resistance to salinity and osmotic pressure. We set out to characterize the Exiguobacterium sp. SH31 strain and describe its phenotypical and genotypical response to osmotic stress. In this context, as a first step to characterize the response to the SH31 strain to salinity and to establish the bases for a molecular study, we proposed to compare its response under three salt conditions (0, 25, and 50 g/l NaCl). Using different physiology, genomic, and transcriptomic approaches, we determined that the bacterium is able to grow properly in a NaCl concentration of up to 50 g/l; however, the best growth rate was observed at 25 g/l. Although the presence of flagella is not affected by salinity, motility was diminished at 25 g/l NaCl and abolished at 50 g/l. Biofilm formation was induced proportionally with increases in salinity, which was expected. These phenotypic results correlated with the expression of related genes: fliG and fliS Motility); opuBA and putP (transport); glnA, proC, gltA, and gbsA (compatible solutes); ywqC, bdlA, luxS y pgaC (biofilm and stress response); and therefore, we conclude that this strain effectively modifies gene expression and physiology in a differential manner when faced with different concentrations of NaCl and these modifications aid survival.",
    keywords = "halotolerant, Chilean Altiplano, Exiguobacterium, extremophile, environmental pressure",
    author = "Francisco Remonsellez and Juan Castro-Severyn and Coral Pardo-Este and Pablo Aguilar and Jonathan Fortt and Cesar Salinas and Sergio Barahona and Joice Leon and Barbara Fuentes and Carlos Areche and Hernandez, {Klaudia L.} and Saavedra, {Claudia P.} and Daniel Aguayo",
    year = "2018",
    month = "9",
    day = "20",
    doi = "10.3389/fmicb.2018.02228",
    language = "English",
    volume = "9",
    journal = "Frontiers in Microbiology",
    issn = "1664-302X",
    publisher = "Frontiers Media S.A.",
    number = "SEP",

    }

    Remonsellez, F, Castro-Severyn, J, Pardo-Este, C, Aguilar, P, Fortt, J, Salinas, C, Barahona, S, Leon, J, Fuentes, B, Areche, C, Hernandez, KL, Saavedra, CP & Aguayo, D 2018, 'Characterization and Salt Response in Recurrent Halotolerant Exiguobacterium sp SH31 Isolated From Sediments of Salar de Huasco, Chilean Altiplano' Frontiers in Microbiology, vol. 9, n.º SEP, 2228. https://doi.org/10.3389/fmicb.2018.02228

    Characterization and Salt Response in Recurrent Halotolerant Exiguobacterium sp SH31 Isolated From Sediments of Salar de Huasco, Chilean Altiplano. / Remonsellez, Francisco; Castro-Severyn, Juan; Pardo-Este, Coral; Aguilar, Pablo; Fortt, Jonathan; Salinas, Cesar; Barahona, Sergio; Leon, Joice; Fuentes, Barbara; Areche, Carlos; Hernandez, Klaudia L.; Saavedra, Claudia P.; Aguayo, Daniel.

    En: Frontiers in Microbiology, Vol. 9, N.º SEP, 2228, 20.09.2018.

    Resultado de la investigación: Article

    TY - JOUR

    T1 - Characterization and Salt Response in Recurrent Halotolerant Exiguobacterium sp SH31 Isolated From Sediments of Salar de Huasco, Chilean Altiplano

    AU - Remonsellez, Francisco

    AU - Castro-Severyn, Juan

    AU - Pardo-Este, Coral

    AU - Aguilar, Pablo

    AU - Fortt, Jonathan

    AU - Salinas, Cesar

    AU - Barahona, Sergio

    AU - Leon, Joice

    AU - Fuentes, Barbara

    AU - Areche, Carlos

    AU - Hernandez, Klaudia L.

    AU - Saavedra, Claudia P.

    AU - Aguayo, Daniel

    PY - 2018/9/20

    Y1 - 2018/9/20

    N2 - Poly-extremophiles microorganisms have the capacity to inhabit hostile environments and can survive several adverse conditions that include as variations in temperature, pH, and salinity, high levels UV light and atmospheric pressure, and even the presence of toxic compounds and the formation of reactive oxygen species (ROS). A halotolerant Exiguobacterium strain was isolated from Salar de Huasco (Chilean Altiplano), a well-known shallow lake area with variable salinity levels, little human intervention, and extreme environmental conditions, which makes it ideal for the study of resistant mechanisms and the evolution of adaptations. This bacterial genus has not been extensively studied, although its cosmopolitan location indicates that it has high levels of plasticity and adaptive capacity. However, to date, there are no studies regarding the tolerance and resistance to salinity and osmotic pressure. We set out to characterize the Exiguobacterium sp. SH31 strain and describe its phenotypical and genotypical response to osmotic stress. In this context, as a first step to characterize the response to the SH31 strain to salinity and to establish the bases for a molecular study, we proposed to compare its response under three salt conditions (0, 25, and 50 g/l NaCl). Using different physiology, genomic, and transcriptomic approaches, we determined that the bacterium is able to grow properly in a NaCl concentration of up to 50 g/l; however, the best growth rate was observed at 25 g/l. Although the presence of flagella is not affected by salinity, motility was diminished at 25 g/l NaCl and abolished at 50 g/l. Biofilm formation was induced proportionally with increases in salinity, which was expected. These phenotypic results correlated with the expression of related genes: fliG and fliS Motility); opuBA and putP (transport); glnA, proC, gltA, and gbsA (compatible solutes); ywqC, bdlA, luxS y pgaC (biofilm and stress response); and therefore, we conclude that this strain effectively modifies gene expression and physiology in a differential manner when faced with different concentrations of NaCl and these modifications aid survival.

    AB - Poly-extremophiles microorganisms have the capacity to inhabit hostile environments and can survive several adverse conditions that include as variations in temperature, pH, and salinity, high levels UV light and atmospheric pressure, and even the presence of toxic compounds and the formation of reactive oxygen species (ROS). A halotolerant Exiguobacterium strain was isolated from Salar de Huasco (Chilean Altiplano), a well-known shallow lake area with variable salinity levels, little human intervention, and extreme environmental conditions, which makes it ideal for the study of resistant mechanisms and the evolution of adaptations. This bacterial genus has not been extensively studied, although its cosmopolitan location indicates that it has high levels of plasticity and adaptive capacity. However, to date, there are no studies regarding the tolerance and resistance to salinity and osmotic pressure. We set out to characterize the Exiguobacterium sp. SH31 strain and describe its phenotypical and genotypical response to osmotic stress. In this context, as a first step to characterize the response to the SH31 strain to salinity and to establish the bases for a molecular study, we proposed to compare its response under three salt conditions (0, 25, and 50 g/l NaCl). Using different physiology, genomic, and transcriptomic approaches, we determined that the bacterium is able to grow properly in a NaCl concentration of up to 50 g/l; however, the best growth rate was observed at 25 g/l. Although the presence of flagella is not affected by salinity, motility was diminished at 25 g/l NaCl and abolished at 50 g/l. Biofilm formation was induced proportionally with increases in salinity, which was expected. These phenotypic results correlated with the expression of related genes: fliG and fliS Motility); opuBA and putP (transport); glnA, proC, gltA, and gbsA (compatible solutes); ywqC, bdlA, luxS y pgaC (biofilm and stress response); and therefore, we conclude that this strain effectively modifies gene expression and physiology in a differential manner when faced with different concentrations of NaCl and these modifications aid survival.

    KW - halotolerant

    KW - Chilean Altiplano

    KW - Exiguobacterium

    KW - extremophile

    KW - environmental pressure

    UR - http://www.scopus.com/inward/record.url?scp=85055106183&partnerID=8YFLogxK

    U2 - 10.3389/fmicb.2018.02228

    DO - 10.3389/fmicb.2018.02228

    M3 - Article

    VL - 9

    JO - Frontiers in Microbiology

    JF - Frontiers in Microbiology

    SN - 1664-302X

    IS - SEP

    M1 - 2228

    ER -