Characterization and pathogenic role of outer membrane vesicles produced by the fish pathogen Piscirickettsia salmonis under in vitro conditions

Cristian Oliver, Karla Valenzuela, Mauricio Hernández, Rodrigo Sandoval, Ronie E. Haro, Ruben Avendaño-Herrera, Juan G. Cárcamo, Maite T. Villar, Antonio Artigues, Rafael Garduño, Alejandro J. Yáñez

Resultado de la investigación: Contribución a una revistaArtículo

14 Citas (Scopus)

Resumen

Piscirickettsia salmonis is one of the major fish pathogens affecting Chilean aquaculture. This Gram-negative bacterium is highly infectious and is the etiological agent of Piscirickettsiosis. Little is currently known about how the virulence factors expressed by P. salmonis are delivered to host cells. However, it is known that several Gram-negative microorganisms constitutively release outer membrane vesicles (OMVs), which have been implicated in the delivery of virulence factors to host cells. In this study, OMVs production by P. salmonis was observed during infection in CHSE-214 cells and during normal growth in liquid media. The OMVs were spherical vesicles ranging in size between 25 and 145 nm. SDS-PAGE analysis demonstrated that the protein profile of the OMVs was similar to the outer membrane protein profile of P. salmonis. Importantly, the bacterial chaperonin Hsp60 was found in the OMVs of P. salmonis by Western-blot and LC-MS/MS analyses. Finally, in vitro infection assays showed that purified OMVs generated a cytopathic effect on CHSE-214 cells, suggesting a role in pathogenesis. Therefore, OMVs might be an important vehicle for delivering effector molecules to host cells during P. salmonis infection.

Idioma originalInglés
Páginas (desde-hasta)94-101
Número de páginas8
PublicaciónVeterinary Microbiology
Volumen184
DOI
EstadoPublicada - 29 feb 2016

Áreas temáticas de ASJC Scopus

  • Microbiología
  • Veterinaria (todo)

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    Oliver, C., Valenzuela, K., Hernández, M., Sandoval, R., Haro, R. E., Avendaño-Herrera, R., Cárcamo, J. G., Villar, M. T., Artigues, A., Garduño, R., & Yáñez, A. J. (2016). Characterization and pathogenic role of outer membrane vesicles produced by the fish pathogen Piscirickettsia salmonis under in vitro conditions. Veterinary Microbiology, 184, 94-101. https://doi.org/10.1016/j.vetmic.2015.09.012