Characterization and pathogenic role of outer membrane vesicles produced by the fish pathogen Piscirickettsia salmonis under in vitro conditions

Cristian Oliver, Karla Valenzuela, Mauricio Hernández, Rodrigo Sandoval, Ronie E. Haro, Ruben Avendaño-Herrera, Juan G. Cárcamo, Maite T. Villar, Antonio Artigues, Rafael Garduño, Alejandro J. Yáñez

Resultado de la investigación: Article

12 Citas (Scopus)

Resumen

Piscirickettsia salmonis is one of the major fish pathogens affecting Chilean aquaculture. This Gram-negative bacterium is highly infectious and is the etiological agent of Piscirickettsiosis. Little is currently known about how the virulence factors expressed by P. salmonis are delivered to host cells. However, it is known that several Gram-negative microorganisms constitutively release outer membrane vesicles (OMVs), which have been implicated in the delivery of virulence factors to host cells. In this study, OMVs production by P. salmonis was observed during infection in CHSE-214 cells and during normal growth in liquid media. The OMVs were spherical vesicles ranging in size between 25 and 145 nm. SDS-PAGE analysis demonstrated that the protein profile of the OMVs was similar to the outer membrane protein profile of P. salmonis. Importantly, the bacterial chaperonin Hsp60 was found in the OMVs of P. salmonis by Western-blot and LC-MS/MS analyses. Finally, in vitro infection assays showed that purified OMVs generated a cytopathic effect on CHSE-214 cells, suggesting a role in pathogenesis. Therefore, OMVs might be an important vehicle for delivering effector molecules to host cells during P. salmonis infection.

Idioma originalEnglish
Páginas (desde-hasta)94-101
Número de páginas8
PublicaciónVeterinary Microbiology
Volumen184
DOI
EstadoPublished - 29 feb 2016

Huella dactilar

Piscirickettsia
Piscirickettsia salmonis
Fishes
Membranes
pathogens
fish
Virulence Factors
cells
Piscirickettsiaceae Infections
virulence
infection
chaperonins
Infection
Chaperonins
cytopathogenicity
outer membrane proteins
etiological agents
Aquaculture
Gram-negative bacteria
In Vitro Techniques

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Citar esto

Oliver, Cristian ; Valenzuela, Karla ; Hernández, Mauricio ; Sandoval, Rodrigo ; Haro, Ronie E. ; Avendaño-Herrera, Ruben ; Cárcamo, Juan G. ; Villar, Maite T. ; Artigues, Antonio ; Garduño, Rafael ; Yáñez, Alejandro J. / Characterization and pathogenic role of outer membrane vesicles produced by the fish pathogen Piscirickettsia salmonis under in vitro conditions. En: Veterinary Microbiology. 2016 ; Vol. 184. pp. 94-101.
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title = "Characterization and pathogenic role of outer membrane vesicles produced by the fish pathogen Piscirickettsia salmonis under in vitro conditions",
abstract = "Piscirickettsia salmonis is one of the major fish pathogens affecting Chilean aquaculture. This Gram-negative bacterium is highly infectious and is the etiological agent of Piscirickettsiosis. Little is currently known about how the virulence factors expressed by P. salmonis are delivered to host cells. However, it is known that several Gram-negative microorganisms constitutively release outer membrane vesicles (OMVs), which have been implicated in the delivery of virulence factors to host cells. In this study, OMVs production by P. salmonis was observed during infection in CHSE-214 cells and during normal growth in liquid media. The OMVs were spherical vesicles ranging in size between 25 and 145 nm. SDS-PAGE analysis demonstrated that the protein profile of the OMVs was similar to the outer membrane protein profile of P. salmonis. Importantly, the bacterial chaperonin Hsp60 was found in the OMVs of P. salmonis by Western-blot and LC-MS/MS analyses. Finally, in vitro infection assays showed that purified OMVs generated a cytopathic effect on CHSE-214 cells, suggesting a role in pathogenesis. Therefore, OMVs might be an important vehicle for delivering effector molecules to host cells during P. salmonis infection.",
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author = "Cristian Oliver and Karla Valenzuela and Mauricio Hern{\'a}ndez and Rodrigo Sandoval and Haro, {Ronie E.} and Ruben Avenda{\~n}o-Herrera and C{\'a}rcamo, {Juan G.} and Villar, {Maite T.} and Antonio Artigues and Rafael Gardu{\~n}o and Y{\'a}{\~n}ez, {Alejandro J.}",
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Oliver, C, Valenzuela, K, Hernández, M, Sandoval, R, Haro, RE, Avendaño-Herrera, R, Cárcamo, JG, Villar, MT, Artigues, A, Garduño, R & Yáñez, AJ 2016, 'Characterization and pathogenic role of outer membrane vesicles produced by the fish pathogen Piscirickettsia salmonis under in vitro conditions', Veterinary Microbiology, vol. 184, pp. 94-101. https://doi.org/10.1016/j.vetmic.2015.09.012

Characterization and pathogenic role of outer membrane vesicles produced by the fish pathogen Piscirickettsia salmonis under in vitro conditions. / Oliver, Cristian; Valenzuela, Karla; Hernández, Mauricio; Sandoval, Rodrigo; Haro, Ronie E.; Avendaño-Herrera, Ruben; Cárcamo, Juan G.; Villar, Maite T.; Artigues, Antonio; Garduño, Rafael; Yáñez, Alejandro J.

En: Veterinary Microbiology, Vol. 184, 29.02.2016, p. 94-101.

Resultado de la investigación: Article

TY - JOUR

T1 - Characterization and pathogenic role of outer membrane vesicles produced by the fish pathogen Piscirickettsia salmonis under in vitro conditions

AU - Oliver, Cristian

AU - Valenzuela, Karla

AU - Hernández, Mauricio

AU - Sandoval, Rodrigo

AU - Haro, Ronie E.

AU - Avendaño-Herrera, Ruben

AU - Cárcamo, Juan G.

AU - Villar, Maite T.

AU - Artigues, Antonio

AU - Garduño, Rafael

AU - Yáñez, Alejandro J.

PY - 2016/2/29

Y1 - 2016/2/29

N2 - Piscirickettsia salmonis is one of the major fish pathogens affecting Chilean aquaculture. This Gram-negative bacterium is highly infectious and is the etiological agent of Piscirickettsiosis. Little is currently known about how the virulence factors expressed by P. salmonis are delivered to host cells. However, it is known that several Gram-negative microorganisms constitutively release outer membrane vesicles (OMVs), which have been implicated in the delivery of virulence factors to host cells. In this study, OMVs production by P. salmonis was observed during infection in CHSE-214 cells and during normal growth in liquid media. The OMVs were spherical vesicles ranging in size between 25 and 145 nm. SDS-PAGE analysis demonstrated that the protein profile of the OMVs was similar to the outer membrane protein profile of P. salmonis. Importantly, the bacterial chaperonin Hsp60 was found in the OMVs of P. salmonis by Western-blot and LC-MS/MS analyses. Finally, in vitro infection assays showed that purified OMVs generated a cytopathic effect on CHSE-214 cells, suggesting a role in pathogenesis. Therefore, OMVs might be an important vehicle for delivering effector molecules to host cells during P. salmonis infection.

AB - Piscirickettsia salmonis is one of the major fish pathogens affecting Chilean aquaculture. This Gram-negative bacterium is highly infectious and is the etiological agent of Piscirickettsiosis. Little is currently known about how the virulence factors expressed by P. salmonis are delivered to host cells. However, it is known that several Gram-negative microorganisms constitutively release outer membrane vesicles (OMVs), which have been implicated in the delivery of virulence factors to host cells. In this study, OMVs production by P. salmonis was observed during infection in CHSE-214 cells and during normal growth in liquid media. The OMVs were spherical vesicles ranging in size between 25 and 145 nm. SDS-PAGE analysis demonstrated that the protein profile of the OMVs was similar to the outer membrane protein profile of P. salmonis. Importantly, the bacterial chaperonin Hsp60 was found in the OMVs of P. salmonis by Western-blot and LC-MS/MS analyses. Finally, in vitro infection assays showed that purified OMVs generated a cytopathic effect on CHSE-214 cells, suggesting a role in pathogenesis. Therefore, OMVs might be an important vehicle for delivering effector molecules to host cells during P. salmonis infection.

KW - Hsp60

KW - Outer membrane vesicles (OMV)

KW - Piscirickettsia salmonis

KW - Piscirickettsiosis

KW - Virulence

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