Characteristics of monoclonal antibodies against Piscirickettsia salmonis

A. Jamett, J. Aguayo, A. Miquel, I. Muller, R. Arriagada, M. I. Becker, P. Valenzuela, L. O. Burzio

Resultado de la investigación: Contribución a una revistaArtículo

19 Citas (Scopus)

Resumen

A panel of 28 monoclonal antibodies against Piscirickettsia salmonis was produced using a purified fraction of the bacterium. To determine their specificity to the pathogen, the antibodies were assayed by ELISA and indirect immunofluorescence microscopy. Six monoclonal antibodies were selected based on their strong reaction against P. salmonis and absence of cross-reactivity with other common fish pathogens. Western blot analysis showed that the antibodies reacted to several antigens of P. salmonis. Immunofluorescence assays revealed that these antibodies reacted with the same specificity to different isolates of P. salmonis obtained from the south of Chile. This panel of monoclonal antibodies represents an important tool to develop simple, rapid, sensitive and highly specific methods for the detection of the pathogen and diagnosis of the disease.

Idioma originalInglés
Páginas (desde-hasta)205-215
Número de páginas11
PublicaciónJournal of Fish Diseases
Volumen24
N.º4
DOI
EstadoPublicada - 2001

Áreas temáticas de ASJC Scopus

  • Ciencias acuáticas
  • Veterinaria (todo)
  • Veterinaria (miscelánea)

Huella Profundice en los temas de investigación de 'Characteristics of monoclonal antibodies against Piscirickettsia salmonis'. En conjunto forman una huella única.

  • Citar esto

    Jamett, A., Aguayo, J., Miquel, A., Muller, I., Arriagada, R., Becker, M. I., Valenzuela, P., & Burzio, L. O. (2001). Characteristics of monoclonal antibodies against Piscirickettsia salmonis. Journal of Fish Diseases, 24(4), 205-215. https://doi.org/10.1046/j.1365-2761.2001.00285.x