Characteristics of monoclonal antibodies against Piscirickettsia salmonis

A. Jamett, J. Aguayo, A. Miquel, I. Muller, R. Arriagada, M. I. Becker, P. Valenzuela, L. O. Burzio

Resultado de la investigación: Article

19 Citas (Scopus)

Resumen

A panel of 28 monoclonal antibodies against Piscirickettsia salmonis was produced using a purified fraction of the bacterium. To determine their specificity to the pathogen, the antibodies were assayed by ELISA and indirect immunofluorescence microscopy. Six monoclonal antibodies were selected based on their strong reaction against P. salmonis and absence of cross-reactivity with other common fish pathogens. Western blot analysis showed that the antibodies reacted to several antigens of P. salmonis. Immunofluorescence assays revealed that these antibodies reacted with the same specificity to different isolates of P. salmonis obtained from the south of Chile. This panel of monoclonal antibodies represents an important tool to develop simple, rapid, sensitive and highly specific methods for the detection of the pathogen and diagnosis of the disease.

Idioma originalEnglish
Páginas (desde-hasta)205-215
Número de páginas11
PublicaciónJournal of Fish Diseases
Volumen24
N.º4
DOI
EstadoPublished - 2001

Huella dactilar

Piscirickettsia
Piscirickettsia salmonis
antibody
monoclonal antibodies
Monoclonal Antibodies
Antibodies
antibodies
pathogen
microbial detection
Chile
Indirect Fluorescent Antibody Technique
Fluorescence Microscopy
Fluorescent Antibody Technique
pathogens
Fishes
disease diagnosis
fluorescence microscopy
Western Blotting
Enzyme-Linked Immunosorbent Assay
cross reaction

ASJC Scopus subject areas

  • Aquatic Science
  • veterinary(all)
  • veterinary (miscalleneous)

Citar esto

Jamett, A., Aguayo, J., Miquel, A., Muller, I., Arriagada, R., Becker, M. I., ... Burzio, L. O. (2001). Characteristics of monoclonal antibodies against Piscirickettsia salmonis. Journal of Fish Diseases, 24(4), 205-215. https://doi.org/10.1046/j.1365-2761.2001.00285.x
Jamett, A. ; Aguayo, J. ; Miquel, A. ; Muller, I. ; Arriagada, R. ; Becker, M. I. ; Valenzuela, P. ; Burzio, L. O. / Characteristics of monoclonal antibodies against Piscirickettsia salmonis. En: Journal of Fish Diseases. 2001 ; Vol. 24, N.º 4. pp. 205-215.
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Jamett, A, Aguayo, J, Miquel, A, Muller, I, Arriagada, R, Becker, MI, Valenzuela, P & Burzio, LO 2001, 'Characteristics of monoclonal antibodies against Piscirickettsia salmonis', Journal of Fish Diseases, vol. 24, n.º 4, pp. 205-215. https://doi.org/10.1046/j.1365-2761.2001.00285.x

Characteristics of monoclonal antibodies against Piscirickettsia salmonis. / Jamett, A.; Aguayo, J.; Miquel, A.; Muller, I.; Arriagada, R.; Becker, M. I.; Valenzuela, P.; Burzio, L. O.

En: Journal of Fish Diseases, Vol. 24, N.º 4, 2001, p. 205-215.

Resultado de la investigación: Article

TY - JOUR

T1 - Characteristics of monoclonal antibodies against Piscirickettsia salmonis

AU - Jamett, A.

AU - Aguayo, J.

AU - Miquel, A.

AU - Muller, I.

AU - Arriagada, R.

AU - Becker, M. I.

AU - Valenzuela, P.

AU - Burzio, L. O.

PY - 2001

Y1 - 2001

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AB - A panel of 28 monoclonal antibodies against Piscirickettsia salmonis was produced using a purified fraction of the bacterium. To determine their specificity to the pathogen, the antibodies were assayed by ELISA and indirect immunofluorescence microscopy. Six monoclonal antibodies were selected based on their strong reaction against P. salmonis and absence of cross-reactivity with other common fish pathogens. Western blot analysis showed that the antibodies reacted to several antigens of P. salmonis. Immunofluorescence assays revealed that these antibodies reacted with the same specificity to different isolates of P. salmonis obtained from the south of Chile. This panel of monoclonal antibodies represents an important tool to develop simple, rapid, sensitive and highly specific methods for the detection of the pathogen and diagnosis of the disease.

KW - Immunofluorescence

KW - Monoclonal antibodies

KW - Piscirickettsia salmonis

KW - Piscirickettsiosis

KW - Salmon

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