Changes in lipopolysaccharide profile of Porphyromonas gingivalis clinical isolates correlate with changes in colony morphology and polymyxin B resistance

Leonor Díaz, Anilei Hoare, Cristopher Soto, Isaac Bugueño, Nora Silva, Nicolás Dutzan, Darna Venegas, Daniela Salinas, José Manuel Pérez-Donoso, Jorge Gamonal, Denisse Bravo

Resultado de la investigación: Article

8 Citas (Scopus)

Resumen

Virulence factors on the surface of Porphyromonas gingivalis constitute the first line of interaction with host cells and contribute to immune modulation and periodontitis progression. In order to characterize surface virulence factors present on P.gingivalis, we obtained clinical isolates from healthy and periodontitis subjects and compared them with reference strains. Colony morphology, aggregation in liquid medium, surface charge, membrane permeability to bactericidal compounds, novobiocin and polymyxin B resistance, capsule presence and lipopolysaccharide (LPS) profiles were evaluated.By comparing isolates from healthy and periodontitis subjects, differences in colony morphology and aggregation in liquid culture were found; the latter being similar to two reference strains. These differences were not a consequence of variations in bacterial surface charge. Furthermore, isolates also presented differences in polymyxin B and novobiocin resistance; isolates from healthy subjects were susceptible to polymyxin B and resistant to novobiocin and, in contrast, isolates from periodontitis subjects were resistant to polymyxin B and susceptible to novobiocin. These changes in antimicrobial resistance levels correlate with variations in LPS profiles, since -unlike periodontitis isolates-isolates from healthy samples synthesize LPS molecules lacking both O-antigen moieties and anionic polysaccharide. Additionally, this phenotype correlated with the absence of O-antigen ligase activity.Altogether, our results reveal novel variations on surface components of P.gingivalis isolates obtained from healthy and periodontitis subjects that could be associated with differences in bacterial virulence and periodontitis progression.

Idioma originalEnglish
Páginas (desde-hasta)25-32
Número de páginas8
PublicaciónAnaerobe
Volumen33
DOI
EstadoPublished - 1 jun 2015

Huella dactilar

Polymyxin B
Porphyromonas gingivalis
Periodontitis
Lipopolysaccharides
Novobiocin
Healthy Volunteers
O Antigens
Virulence Factors
Ligases
Capsules
Polysaccharides
Virulence
Permeability
Phenotype
Membranes

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Citar esto

Díaz, Leonor ; Hoare, Anilei ; Soto, Cristopher ; Bugueño, Isaac ; Silva, Nora ; Dutzan, Nicolás ; Venegas, Darna ; Salinas, Daniela ; Pérez-Donoso, José Manuel ; Gamonal, Jorge ; Bravo, Denisse. / Changes in lipopolysaccharide profile of Porphyromonas gingivalis clinical isolates correlate with changes in colony morphology and polymyxin B resistance. En: Anaerobe. 2015 ; Vol. 33. pp. 25-32.
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title = "Changes in lipopolysaccharide profile of Porphyromonas gingivalis clinical isolates correlate with changes in colony morphology and polymyxin B resistance",
abstract = "Virulence factors on the surface of Porphyromonas gingivalis constitute the first line of interaction with host cells and contribute to immune modulation and periodontitis progression. In order to characterize surface virulence factors present on P.gingivalis, we obtained clinical isolates from healthy and periodontitis subjects and compared them with reference strains. Colony morphology, aggregation in liquid medium, surface charge, membrane permeability to bactericidal compounds, novobiocin and polymyxin B resistance, capsule presence and lipopolysaccharide (LPS) profiles were evaluated.By comparing isolates from healthy and periodontitis subjects, differences in colony morphology and aggregation in liquid culture were found; the latter being similar to two reference strains. These differences were not a consequence of variations in bacterial surface charge. Furthermore, isolates also presented differences in polymyxin B and novobiocin resistance; isolates from healthy subjects were susceptible to polymyxin B and resistant to novobiocin and, in contrast, isolates from periodontitis subjects were resistant to polymyxin B and susceptible to novobiocin. These changes in antimicrobial resistance levels correlate with variations in LPS profiles, since -unlike periodontitis isolates-isolates from healthy samples synthesize LPS molecules lacking both O-antigen moieties and anionic polysaccharide. Additionally, this phenotype correlated with the absence of O-antigen ligase activity.Altogether, our results reveal novel variations on surface components of P.gingivalis isolates obtained from healthy and periodontitis subjects that could be associated with differences in bacterial virulence and periodontitis progression.",
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Changes in lipopolysaccharide profile of Porphyromonas gingivalis clinical isolates correlate with changes in colony morphology and polymyxin B resistance. / Díaz, Leonor; Hoare, Anilei; Soto, Cristopher; Bugueño, Isaac; Silva, Nora; Dutzan, Nicolás; Venegas, Darna; Salinas, Daniela; Pérez-Donoso, José Manuel; Gamonal, Jorge; Bravo, Denisse.

En: Anaerobe, Vol. 33, 01.06.2015, p. 25-32.

Resultado de la investigación: Article

TY - JOUR

T1 - Changes in lipopolysaccharide profile of Porphyromonas gingivalis clinical isolates correlate with changes in colony morphology and polymyxin B resistance

AU - Díaz, Leonor

AU - Hoare, Anilei

AU - Soto, Cristopher

AU - Bugueño, Isaac

AU - Silva, Nora

AU - Dutzan, Nicolás

AU - Venegas, Darna

AU - Salinas, Daniela

AU - Pérez-Donoso, José Manuel

AU - Gamonal, Jorge

AU - Bravo, Denisse

PY - 2015/6/1

Y1 - 2015/6/1

N2 - Virulence factors on the surface of Porphyromonas gingivalis constitute the first line of interaction with host cells and contribute to immune modulation and periodontitis progression. In order to characterize surface virulence factors present on P.gingivalis, we obtained clinical isolates from healthy and periodontitis subjects and compared them with reference strains. Colony morphology, aggregation in liquid medium, surface charge, membrane permeability to bactericidal compounds, novobiocin and polymyxin B resistance, capsule presence and lipopolysaccharide (LPS) profiles were evaluated.By comparing isolates from healthy and periodontitis subjects, differences in colony morphology and aggregation in liquid culture were found; the latter being similar to two reference strains. These differences were not a consequence of variations in bacterial surface charge. Furthermore, isolates also presented differences in polymyxin B and novobiocin resistance; isolates from healthy subjects were susceptible to polymyxin B and resistant to novobiocin and, in contrast, isolates from periodontitis subjects were resistant to polymyxin B and susceptible to novobiocin. These changes in antimicrobial resistance levels correlate with variations in LPS profiles, since -unlike periodontitis isolates-isolates from healthy samples synthesize LPS molecules lacking both O-antigen moieties and anionic polysaccharide. Additionally, this phenotype correlated with the absence of O-antigen ligase activity.Altogether, our results reveal novel variations on surface components of P.gingivalis isolates obtained from healthy and periodontitis subjects that could be associated with differences in bacterial virulence and periodontitis progression.

AB - Virulence factors on the surface of Porphyromonas gingivalis constitute the first line of interaction with host cells and contribute to immune modulation and periodontitis progression. In order to characterize surface virulence factors present on P.gingivalis, we obtained clinical isolates from healthy and periodontitis subjects and compared them with reference strains. Colony morphology, aggregation in liquid medium, surface charge, membrane permeability to bactericidal compounds, novobiocin and polymyxin B resistance, capsule presence and lipopolysaccharide (LPS) profiles were evaluated.By comparing isolates from healthy and periodontitis subjects, differences in colony morphology and aggregation in liquid culture were found; the latter being similar to two reference strains. These differences were not a consequence of variations in bacterial surface charge. Furthermore, isolates also presented differences in polymyxin B and novobiocin resistance; isolates from healthy subjects were susceptible to polymyxin B and resistant to novobiocin and, in contrast, isolates from periodontitis subjects were resistant to polymyxin B and susceptible to novobiocin. These changes in antimicrobial resistance levels correlate with variations in LPS profiles, since -unlike periodontitis isolates-isolates from healthy samples synthesize LPS molecules lacking both O-antigen moieties and anionic polysaccharide. Additionally, this phenotype correlated with the absence of O-antigen ligase activity.Altogether, our results reveal novel variations on surface components of P.gingivalis isolates obtained from healthy and periodontitis subjects that could be associated with differences in bacterial virulence and periodontitis progression.

KW - Chronic periodontitis

KW - Clinical isolates

KW - Lipopolysaccharide

KW - Polymyxin B

KW - Porphyromonas gingivalis

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