Cell internalization kinetics and surface charge accessibility of surface-modified PAMAM dendrimers

Carola F. Díaz, Diego L. Cifuentes, Maximiliano Oyarzún, Verónica A. Jiménez, Leonardo Guzmán

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

Resumen

Surface-modified PAMAM dendrimers have important applications in drug delivery, yet a gap remains about the role that surface functionalization plays on their cell internalization capacity. We examined the cell internalization kinetics of PAMAM dendrimers that were surface-modified with acetyl, folate and poly(ethylene glycol), as model functional groups differing in size, charge, and chemical functionality. Dendrimers with 25% functionalization were internalized by HEK cells, but with slower rates and lower maximum uptakes than the native dendrimer between 1-6 h of incubation. Dendrimers with 50% functionalization exhibited negligible internalization capacities at all incubation times. Molecular dynamics simulations revealed that the solvent accessibility of the cationic surface charges is a key factor affecting cell internalization, unlike the total charge, functionality or size of surface-modified PAMAM dendrimers. These findings provide valuable insights to assist the design of PAMAM-based systems for drug delivery applications.

Idioma originalInglés
Páginas (desde-hasta)7782-7790
Número de páginas9
PublicaciónOrganic and Biomolecular Chemistry
Volumen21
N.º38
DOI
EstadoEn prensa - 2023

Áreas temáticas de ASJC Scopus

  • Bioquímica
  • Química física y teórica
  • Química orgánica

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