TY - JOUR
T1 - Cell-free synthesis of acetylcholine receptor polypeptides
AU - Mendez, Bernadita
AU - Valenzuela, Pablo
AU - Martial, Joseph A.
AU - Baxter, John D.
PY - 1980
Y1 - 1980
N2 - Messenger RNA coding for acetylcholine receptor peptides has been identified. This polyadenylate [poly(A)+]RNA from Torpedo californica directs, in a cell-free system, the synthesis of peptides 60,000, 51,000, 49,000 41,000, and 35,000 daltons which account for approximately 2 percent of the total synthesized proteins. The results suggest that several different messenger RNA's code for the receptor subunits. These proteins react specifically to antiserum to native acteylcholine receptor, suggesting that the primary translational product has conformational features similar to the native receptor. Further, the results support the idea that there is posttranslational modification of receptor subunits as the molecular weights of the cell-free synthesized proteins differ from those of purified receptor subunits.
AB - Messenger RNA coding for acetylcholine receptor peptides has been identified. This polyadenylate [poly(A)+]RNA from Torpedo californica directs, in a cell-free system, the synthesis of peptides 60,000, 51,000, 49,000 41,000, and 35,000 daltons which account for approximately 2 percent of the total synthesized proteins. The results suggest that several different messenger RNA's code for the receptor subunits. These proteins react specifically to antiserum to native acteylcholine receptor, suggesting that the primary translational product has conformational features similar to the native receptor. Further, the results support the idea that there is posttranslational modification of receptor subunits as the molecular weights of the cell-free synthesized proteins differ from those of purified receptor subunits.
UR - http://www.scopus.com/inward/record.url?scp=0019174015&partnerID=8YFLogxK
U2 - 10.1126/science.7394526
DO - 10.1126/science.7394526
M3 - Article
C2 - 7394526
AN - SCOPUS:0019174015
SN - 0036-8075
VL - 209
SP - 695
EP - 697
JO - Science
JF - Science
IS - 4457
ER -