Cell-free synthesis of acetylcholine receptor polypeptides

Bernadita Mendez, Pablo Valenzuela, Joseph A. Martial, John D. Baxter

Resultado de la investigación: Article

22 Citas (Scopus)

Resumen

Messenger RNA coding for acetylcholine receptor peptides has been identified. This polyadenylate [poly(A)+]RNA from Torpedo californica directs, in a cell-free system, the synthesis of peptides 60,000, 51,000, 49,000 41,000, and 35,000 daltons which account for approximately 2 percent of the total synthesized proteins. The results suggest that several different messenger RNA's code for the receptor subunits. These proteins react specifically to antiserum to native acteylcholine receptor, suggesting that the primary translational product has conformational features similar to the native receptor. Further, the results support the idea that there is posttranslational modification of receptor subunits as the molecular weights of the cell-free synthesized proteins differ from those of purified receptor subunits.

Idioma originalEnglish
Páginas (desde-hasta)695-697
Número de páginas3
PublicaciónScience
Volumen209
N.º4457
EstadoPublished - 1980

Huella dactilar

Cholinergic Receptors
Messenger RNA
Peptides
Torpedo
Proteins
Cell-Free System
Post Translational Protein Processing
Immune Sera
Molecular Weight

ASJC Scopus subject areas

  • Medicine(all)
  • General

Citar esto

Mendez, B., Valenzuela, P., Martial, J. A., & Baxter, J. D. (1980). Cell-free synthesis of acetylcholine receptor polypeptides. Science, 209(4457), 695-697.
Mendez, Bernadita ; Valenzuela, Pablo ; Martial, Joseph A. ; Baxter, John D. / Cell-free synthesis of acetylcholine receptor polypeptides. En: Science. 1980 ; Vol. 209, N.º 4457. pp. 695-697.
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Mendez, B, Valenzuela, P, Martial, JA & Baxter, JD 1980, 'Cell-free synthesis of acetylcholine receptor polypeptides', Science, vol. 209, n.º 4457, pp. 695-697.

Cell-free synthesis of acetylcholine receptor polypeptides. / Mendez, Bernadita; Valenzuela, Pablo; Martial, Joseph A.; Baxter, John D.

En: Science, Vol. 209, N.º 4457, 1980, p. 695-697.

Resultado de la investigación: Article

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AU - Valenzuela, Pablo

AU - Martial, Joseph A.

AU - Baxter, John D.

PY - 1980

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Mendez B, Valenzuela P, Martial JA, Baxter JD. Cell-free synthesis of acetylcholine receptor polypeptides. Science. 1980;209(4457):695-697.