CD40L - A costimulatory molecule involved in the maturation of antigen presenting cells in Atlantic salmon (Salmo salar)

Leidy X. Lagos, Dimitar B. Iliev, Ronny Helland, Mario Rosemblatt, Jorunn B. Jørgensen

Resultado de la investigación: Article

15 Citas (Scopus)

Resumen

The CD40L/CD40 signalling pathway is critically involved in the final stage of the maturation of DCs. This paper reports the identification and functional characterization of CD40L and CD40 from Atlantic salmon (Salmo salar). Salmon CD40L is a type II membrane-bound protein with a TNF homology domain in its extracellular C-terminal region, while CD40 is a type I membrane-bound receptor with a sequence pattern of four cysteine-rich domains in its extracellular N-terminal region. The salmon CD40L and CD40 were widely expressed, particularly in immune tissues, and while CD40L expression was induced by in vitro stimulation of HKLs with PHA and ConA, CpG increased CD40 expression. A CD40L construct was overexpressed in the CHSE-214 cell line and co-cultivation of the CD40L-CHSE transfectants with HKL induced a rapid and long-lasting upregulation of important costimulatory molecules like CD40, CD83, B7-H1 and the cytokines IL-12p40, IL-10, IL-1β and IFNs, which all are involved in T-helper cell responses. Furthermore, the CD40L transfected cells increased the percentage of HKLs expressing surface MHCIIβ but unlike other APC maturation stimuli, like CpG, they did not reduce the capacity to internalise antigen. Our results provide the first evidence for the existence of a functional CD40L mediated costimulatory pathway in Atlantic salmon.

Idioma originalEnglish
Páginas (desde-hasta)416-430
Número de páginas15
PublicaciónDevelopmental and Comparative Immunology
Volumen38
N.º3
DOI
EstadoPublished - 1 nov 2012

Huella dactilar

Salmo salar
CD40 Ligand
Antigen-Presenting Cells
Salmon
Interleukin-12 Subunit p40
Helper-Inducer T-Lymphocytes
Interleukin-1
Interleukin-10
Cysteine
Membrane Proteins
Up-Regulation
Cytokines
Antigens
Cell Line
Membranes

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

Citar esto

Lagos, Leidy X. ; Iliev, Dimitar B. ; Helland, Ronny ; Rosemblatt, Mario ; Jørgensen, Jorunn B. / CD40L - A costimulatory molecule involved in the maturation of antigen presenting cells in Atlantic salmon (Salmo salar). En: Developmental and Comparative Immunology. 2012 ; Vol. 38, N.º 3. pp. 416-430.
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abstract = "The CD40L/CD40 signalling pathway is critically involved in the final stage of the maturation of DCs. This paper reports the identification and functional characterization of CD40L and CD40 from Atlantic salmon (Salmo salar). Salmon CD40L is a type II membrane-bound protein with a TNF homology domain in its extracellular C-terminal region, while CD40 is a type I membrane-bound receptor with a sequence pattern of four cysteine-rich domains in its extracellular N-terminal region. The salmon CD40L and CD40 were widely expressed, particularly in immune tissues, and while CD40L expression was induced by in vitro stimulation of HKLs with PHA and ConA, CpG increased CD40 expression. A CD40L construct was overexpressed in the CHSE-214 cell line and co-cultivation of the CD40L-CHSE transfectants with HKL induced a rapid and long-lasting upregulation of important costimulatory molecules like CD40, CD83, B7-H1 and the cytokines IL-12p40, IL-10, IL-1β and IFNs, which all are involved in T-helper cell responses. Furthermore, the CD40L transfected cells increased the percentage of HKLs expressing surface MHCIIβ but unlike other APC maturation stimuli, like CpG, they did not reduce the capacity to internalise antigen. Our results provide the first evidence for the existence of a functional CD40L mediated costimulatory pathway in Atlantic salmon.",
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CD40L - A costimulatory molecule involved in the maturation of antigen presenting cells in Atlantic salmon (Salmo salar). / Lagos, Leidy X.; Iliev, Dimitar B.; Helland, Ronny; Rosemblatt, Mario; Jørgensen, Jorunn B.

En: Developmental and Comparative Immunology, Vol. 38, N.º 3, 01.11.2012, p. 416-430.

Resultado de la investigación: Article

TY - JOUR

T1 - CD40L - A costimulatory molecule involved in the maturation of antigen presenting cells in Atlantic salmon (Salmo salar)

AU - Lagos, Leidy X.

AU - Iliev, Dimitar B.

AU - Helland, Ronny

AU - Rosemblatt, Mario

AU - Jørgensen, Jorunn B.

PY - 2012/11/1

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N2 - The CD40L/CD40 signalling pathway is critically involved in the final stage of the maturation of DCs. This paper reports the identification and functional characterization of CD40L and CD40 from Atlantic salmon (Salmo salar). Salmon CD40L is a type II membrane-bound protein with a TNF homology domain in its extracellular C-terminal region, while CD40 is a type I membrane-bound receptor with a sequence pattern of four cysteine-rich domains in its extracellular N-terminal region. The salmon CD40L and CD40 were widely expressed, particularly in immune tissues, and while CD40L expression was induced by in vitro stimulation of HKLs with PHA and ConA, CpG increased CD40 expression. A CD40L construct was overexpressed in the CHSE-214 cell line and co-cultivation of the CD40L-CHSE transfectants with HKL induced a rapid and long-lasting upregulation of important costimulatory molecules like CD40, CD83, B7-H1 and the cytokines IL-12p40, IL-10, IL-1β and IFNs, which all are involved in T-helper cell responses. Furthermore, the CD40L transfected cells increased the percentage of HKLs expressing surface MHCIIβ but unlike other APC maturation stimuli, like CpG, they did not reduce the capacity to internalise antigen. Our results provide the first evidence for the existence of a functional CD40L mediated costimulatory pathway in Atlantic salmon.

AB - The CD40L/CD40 signalling pathway is critically involved in the final stage of the maturation of DCs. This paper reports the identification and functional characterization of CD40L and CD40 from Atlantic salmon (Salmo salar). Salmon CD40L is a type II membrane-bound protein with a TNF homology domain in its extracellular C-terminal region, while CD40 is a type I membrane-bound receptor with a sequence pattern of four cysteine-rich domains in its extracellular N-terminal region. The salmon CD40L and CD40 were widely expressed, particularly in immune tissues, and while CD40L expression was induced by in vitro stimulation of HKLs with PHA and ConA, CpG increased CD40 expression. A CD40L construct was overexpressed in the CHSE-214 cell line and co-cultivation of the CD40L-CHSE transfectants with HKL induced a rapid and long-lasting upregulation of important costimulatory molecules like CD40, CD83, B7-H1 and the cytokines IL-12p40, IL-10, IL-1β and IFNs, which all are involved in T-helper cell responses. Furthermore, the CD40L transfected cells increased the percentage of HKLs expressing surface MHCIIβ but unlike other APC maturation stimuli, like CpG, they did not reduce the capacity to internalise antigen. Our results provide the first evidence for the existence of a functional CD40L mediated costimulatory pathway in Atlantic salmon.

KW - Antigen presenting cells

KW - Atlantic salmon

KW - CD40 ligand

KW - Costimulation

KW - Cytokines

KW - Maturation markers

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