CD26, adenosine deaminase, and adenosine receptors mediate costimulatory signals in the immunological synapse

R. Pacheco, J. M. Martinez-Navio, M. Lejeune, N. Climent, H. Oliva, J. M. Gatell, T. Gallart, J. Mallol, C. Lluis, R. Franco

Resultado de la investigación: Article

158 Citas (Scopus)

Resumen

Adenosine deaminase (ADA), a protein whose deficit leads to severe combined immunodeficiency, binds to the cell surface by means of either CD26, A 1 adenosine receptors, or A2B adenosine receptors. The physiological role of these interactions is not well understood. Our results show that by a 3-fold reduction in the EC50 for the antigen, ADA potentiated T cell proliferation in autologous cocultures with antigen-pulsed immature or mature dendritic cells. Costimulation was not due to the enzymatic activity but to the interaction of ADA-CD26 complexes in T cells with an ADA-anchoring protein in dendritic cells. From colocalization studies, it is deduced that ADA colocalizing with adenosine receptors on dendritic cells interact with CD26 expressed on lymphocytes. This costimulatory signal in the immunological synapse leads to a marked increase (3- to 34-fold) in the production of the T helper 1 and proimmflamatory cytokines IFN-γ, TNF-α, and IL-6.

Idioma originalEnglish
Páginas (desde-hasta)9583-9588
Número de páginas6
PublicaciónProceedings of the National Academy of Sciences of the United States of America
Volumen102
N.º27
DOI
EstadoPublished - 5 jul 2005

Huella dactilar

Immunological Synapses
Purinergic P1 Receptors
Adenosine Deaminase
Dendritic Cells
Adenosine A2B Receptors
T-Lymphocytes
Antigens
Severe Combined Immunodeficiency
Coculture Techniques
Interleukin-6
Proteins
Cell Proliferation
Lymphocytes
Cytokines

ASJC Scopus subject areas

  • Genetics
  • General

Citar esto

Pacheco, R. ; Martinez-Navio, J. M. ; Lejeune, M. ; Climent, N. ; Oliva, H. ; Gatell, J. M. ; Gallart, T. ; Mallol, J. ; Lluis, C. ; Franco, R. / CD26, adenosine deaminase, and adenosine receptors mediate costimulatory signals in the immunological synapse. En: Proceedings of the National Academy of Sciences of the United States of America. 2005 ; Vol. 102, N.º 27. pp. 9583-9588.
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title = "CD26, adenosine deaminase, and adenosine receptors mediate costimulatory signals in the immunological synapse",
abstract = "Adenosine deaminase (ADA), a protein whose deficit leads to severe combined immunodeficiency, binds to the cell surface by means of either CD26, A 1 adenosine receptors, or A2B adenosine receptors. The physiological role of these interactions is not well understood. Our results show that by a 3-fold reduction in the EC50 for the antigen, ADA potentiated T cell proliferation in autologous cocultures with antigen-pulsed immature or mature dendritic cells. Costimulation was not due to the enzymatic activity but to the interaction of ADA-CD26 complexes in T cells with an ADA-anchoring protein in dendritic cells. From colocalization studies, it is deduced that ADA colocalizing with adenosine receptors on dendritic cells interact with CD26 expressed on lymphocytes. This costimulatory signal in the immunological synapse leads to a marked increase (3- to 34-fold) in the production of the T helper 1 and proimmflamatory cytokines IFN-γ, TNF-α, and IL-6.",
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Pacheco, R, Martinez-Navio, JM, Lejeune, M, Climent, N, Oliva, H, Gatell, JM, Gallart, T, Mallol, J, Lluis, C & Franco, R 2005, 'CD26, adenosine deaminase, and adenosine receptors mediate costimulatory signals in the immunological synapse', Proceedings of the National Academy of Sciences of the United States of America, vol. 102, n.º 27, pp. 9583-9588. https://doi.org/10.1073/pnas.0501050102

CD26, adenosine deaminase, and adenosine receptors mediate costimulatory signals in the immunological synapse. / Pacheco, R.; Martinez-Navio, J. M.; Lejeune, M.; Climent, N.; Oliva, H.; Gatell, J. M.; Gallart, T.; Mallol, J.; Lluis, C.; Franco, R.

En: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, N.º 27, 05.07.2005, p. 9583-9588.

Resultado de la investigación: Article

TY - JOUR

T1 - CD26, adenosine deaminase, and adenosine receptors mediate costimulatory signals in the immunological synapse

AU - Pacheco, R.

AU - Martinez-Navio, J. M.

AU - Lejeune, M.

AU - Climent, N.

AU - Oliva, H.

AU - Gatell, J. M.

AU - Gallart, T.

AU - Mallol, J.

AU - Lluis, C.

AU - Franco, R.

PY - 2005/7/5

Y1 - 2005/7/5

N2 - Adenosine deaminase (ADA), a protein whose deficit leads to severe combined immunodeficiency, binds to the cell surface by means of either CD26, A 1 adenosine receptors, or A2B adenosine receptors. The physiological role of these interactions is not well understood. Our results show that by a 3-fold reduction in the EC50 for the antigen, ADA potentiated T cell proliferation in autologous cocultures with antigen-pulsed immature or mature dendritic cells. Costimulation was not due to the enzymatic activity but to the interaction of ADA-CD26 complexes in T cells with an ADA-anchoring protein in dendritic cells. From colocalization studies, it is deduced that ADA colocalizing with adenosine receptors on dendritic cells interact with CD26 expressed on lymphocytes. This costimulatory signal in the immunological synapse leads to a marked increase (3- to 34-fold) in the production of the T helper 1 and proimmflamatory cytokines IFN-γ, TNF-α, and IL-6.

AB - Adenosine deaminase (ADA), a protein whose deficit leads to severe combined immunodeficiency, binds to the cell surface by means of either CD26, A 1 adenosine receptors, or A2B adenosine receptors. The physiological role of these interactions is not well understood. Our results show that by a 3-fold reduction in the EC50 for the antigen, ADA potentiated T cell proliferation in autologous cocultures with antigen-pulsed immature or mature dendritic cells. Costimulation was not due to the enzymatic activity but to the interaction of ADA-CD26 complexes in T cells with an ADA-anchoring protein in dendritic cells. From colocalization studies, it is deduced that ADA colocalizing with adenosine receptors on dendritic cells interact with CD26 expressed on lymphocytes. This costimulatory signal in the immunological synapse leads to a marked increase (3- to 34-fold) in the production of the T helper 1 and proimmflamatory cytokines IFN-γ, TNF-α, and IL-6.

KW - Adenosine deaminase

KW - Costimulation

KW - Immunosynapse

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U2 - 10.1073/pnas.0501050102

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JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

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