TY - JOUR
T1 - Caracterización y sensibilidad antimicrobiana de Staphylococcus coagulasa-positivos aislados en un hospital clínico veterinario en Chile
AU - Abusleme, Francisco
AU - Galarce, Nicolás
AU - Quezada-Aguiluz, Mario
AU - Iragüen, Daniela
AU - González-Rocha, Gerardo
N1 - Funding Information:
The authors appreciate the kind collaboration of all staff of Laboratorio de Investigaci?n en Agentes Antibacterianos at Facultad de Ciencias Biol?gicas, Universidad de Concepci?n, and the Laboratorio de Microbiolog?a Cl?nica Veterinaria of the Facultad de Ciencias Veterinarias y Pecuarias of the Universidad de Chile (FAVET). This study was financed by the Conicyt National Doctorate Grant No. 21141033-2014, and by the ANID Millennium Science Initiative/Millennium Initiative for Collaborative Research on Bacterial Resistance, MICROB-R, NCN17_081-2020.
Funding Information:
The authors appreciate the kind collaboration of all staff of Laboratorio de Investigación en Agentes Antibacterianos at Facultad de Ciencias Biológicas, Universidad de Concepción, and the Laboratorio de Microbiología Clínica Veterinaria of the Facultad de Ciencias Veterinarias y Pecuarias of the Universidad de Chile (FAVET). This study was financed by the Conicyt National Doctorate Grant No. 21141033-2014 , and by the ANID Millennium Science Initiative/Millennium Initiative for Collaborative Research on Bacterial Resistance , MICROB-R, NCN17_081-2020 .
Publisher Copyright:
© 2022 Asociación Argentina de Microbiología
PY - 2022
Y1 - 2022
N2 - There is limited information about the prevalence and antimicrobial susceptibility of coagulase-positive Staphylococcus (CoPS) strains in veterinary settings in Chile. The aim of this observational study was to identify and characterize CoPS strains from dogs, owners, veterinary professionals and surfaces in a veterinary teaching hospital at Universidad de Chile to determine the presence of methicillin-resistant strains and evaluate the genetic relationship among the strains. Veterinarians (n = 24), surfaces (n = 10), and healthy dogs (n = 40) and their respective owners (n = 40) were sampled for CoPS. Isolates were identified by PCR and antimicrobial susceptibility was assessed by the disk diffusion method and MIC. The presence of the mecA gene was evaluated by PCR, and the genetic relationship among the strains was established by PFGE. A total of 45 CoPS strains were obtained, eight from veterinary professionals, three from hospital surfaces, eight from owners and 26 from dogs. Nine of the strains were resistant to methicillin (20%), and all of them carried the mecA gene. A high percentage of the strains was resistant to clindamycin (33.3%). Additionally, the isolated CoPS showed high genetic diversity. This study suggests that veterinarians are in high risk of harboring methicillin-resistant CoPS (25% versus 2.5% from owners) and our results provide evidence that clindamycin could not be an empiric alternative for CoPS in the analyzed hospital. This is the first report of methicillin-resistant CoPS in veterinary settings in Chile, considering humans, pets and surfaces.
AB - There is limited information about the prevalence and antimicrobial susceptibility of coagulase-positive Staphylococcus (CoPS) strains in veterinary settings in Chile. The aim of this observational study was to identify and characterize CoPS strains from dogs, owners, veterinary professionals and surfaces in a veterinary teaching hospital at Universidad de Chile to determine the presence of methicillin-resistant strains and evaluate the genetic relationship among the strains. Veterinarians (n = 24), surfaces (n = 10), and healthy dogs (n = 40) and their respective owners (n = 40) were sampled for CoPS. Isolates were identified by PCR and antimicrobial susceptibility was assessed by the disk diffusion method and MIC. The presence of the mecA gene was evaluated by PCR, and the genetic relationship among the strains was established by PFGE. A total of 45 CoPS strains were obtained, eight from veterinary professionals, three from hospital surfaces, eight from owners and 26 from dogs. Nine of the strains were resistant to methicillin (20%), and all of them carried the mecA gene. A high percentage of the strains was resistant to clindamycin (33.3%). Additionally, the isolated CoPS showed high genetic diversity. This study suggests that veterinarians are in high risk of harboring methicillin-resistant CoPS (25% versus 2.5% from owners) and our results provide evidence that clindamycin could not be an empiric alternative for CoPS in the analyzed hospital. This is the first report of methicillin-resistant CoPS in veterinary settings in Chile, considering humans, pets and surfaces.
KW - Antimicrobial resistance
KW - Methicillin-resistant Staphylococcus aureus
KW - Methicillin-resistant Staphylococcus pseudintermedius
KW - Pets
UR - http://www.scopus.com/inward/record.url?scp=85126837567&partnerID=8YFLogxK
U2 - 10.1016/j.ram.2021.12.001
DO - 10.1016/j.ram.2021.12.001
M3 - Artículo
AN - SCOPUS:85126837567
SN - 0325-7541
VL - 54
SP - 192
EP - 202
JO - Revista Argentina de Microbiologia
JF - Revista Argentina de Microbiologia
IS - 3
ER -