Caracterización molecular de mecanismos de resistencia a cloranfenicol en cepas de Shigella flexneri aisladas en niños chilenos con diarrea aguda

Mauricio Farfán U, Oscar Flores G, Niurka Navarro G, Valeria Prado J, Guido Mora L, Cecilia Toro U

Resultado de la investigación: Article

4 Citas (Scopus)

Resumen

Background: Chloramphenicol is one of the therapeutic options for shigellosis, but resistance to this antimicrobial is increasing. Aim: To characterize molecular mechanisms conferring resistance to chloramphenicol (CmR) in Shigella flexneri strains isolated from Chilean children with acute diarrhea. Material and methods: Thirty one Shigella filexneri strains, including 22 with the CmR phenotype were analyzed. Strains were tested for antimicrobial susceptibility by plate dilution and for the presence of an internal fragment of the cat gene encoding for chloramphenicol o-acetyl-transferase, by polymerase chain reaction and Southern blot analysis. Results: All CmR strains had a minimal inhibitory concentration over 64 μg/ml and amplified the internal fragment of the cat gene. Southern blot analyses indicated that this gene was located in the bacterial chromosome. Conclusions: Resistance to chloramphenicol in this group of Shigella flexneri strains was mediated by a chromosomally located cat gene.

Idioma originalSpanish
Páginas (desde-hasta)275-280
Número de páginas6
PublicaciónRevista Medica de Chile
Volumen130
N.º3
EstadoPublished - mar 2002

Huella dactilar

Shigella flexneri
Chloramphenicol
Diarrhea
Chloramphenicol Resistance
Cats
Southern Blotting
Genes
Bacterial Chromosomes
Bacillary Dysentery
Shigella
Transferases
Phenotype
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Medicine(all)

Citar esto

Farfán U, Mauricio ; Flores G, Oscar ; Navarro G, Niurka ; Prado J, Valeria ; Mora L, Guido ; Toro U, Cecilia. / Caracterización molecular de mecanismos de resistencia a cloranfenicol en cepas de Shigella flexneri aisladas en niños chilenos con diarrea aguda. En: Revista Medica de Chile. 2002 ; Vol. 130, N.º 3. pp. 275-280.
@article{3f5e13c74284459b89f28739c07114de,
title = "Caracterizaci{\'o}n molecular de mecanismos de resistencia a cloranfenicol en cepas de Shigella flexneri aisladas en ni{\~n}os chilenos con diarrea aguda",
abstract = "Background: Chloramphenicol is one of the therapeutic options for shigellosis, but resistance to this antimicrobial is increasing. Aim: To characterize molecular mechanisms conferring resistance to chloramphenicol (CmR) in Shigella flexneri strains isolated from Chilean children with acute diarrhea. Material and methods: Thirty one Shigella filexneri strains, including 22 with the CmR phenotype were analyzed. Strains were tested for antimicrobial susceptibility by plate dilution and for the presence of an internal fragment of the cat gene encoding for chloramphenicol o-acetyl-transferase, by polymerase chain reaction and Southern blot analysis. Results: All CmR strains had a minimal inhibitory concentration over 64 μg/ml and amplified the internal fragment of the cat gene. Southern blot analyses indicated that this gene was located in the bacterial chromosome. Conclusions: Resistance to chloramphenicol in this group of Shigella flexneri strains was mediated by a chromosomally located cat gene.",
keywords = "Chloramphenicol o-acetyl-transferase, Chloramphenicol resistance, Genes, cat, Shigella flexneri",
author = "{Farf{\'a}n U}, Mauricio and {Flores G}, Oscar and {Navarro G}, Niurka and {Prado J}, Valeria and {Mora L}, Guido and {Toro U}, Cecilia",
year = "2002",
month = "3",
language = "Spanish",
volume = "130",
pages = "275--280",
journal = "Revista Medica de Chile",
issn = "0034-9887",
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}

Farfán U, M, Flores G, O, Navarro G, N, Prado J, V, Mora L, G & Toro U, C 2002, 'Caracterización molecular de mecanismos de resistencia a cloranfenicol en cepas de Shigella flexneri aisladas en niños chilenos con diarrea aguda', Revista Medica de Chile, vol. 130, n.º 3, pp. 275-280.

Caracterización molecular de mecanismos de resistencia a cloranfenicol en cepas de Shigella flexneri aisladas en niños chilenos con diarrea aguda. / Farfán U, Mauricio; Flores G, Oscar; Navarro G, Niurka; Prado J, Valeria; Mora L, Guido; Toro U, Cecilia.

En: Revista Medica de Chile, Vol. 130, N.º 3, 03.2002, p. 275-280.

Resultado de la investigación: Article

TY - JOUR

T1 - Caracterización molecular de mecanismos de resistencia a cloranfenicol en cepas de Shigella flexneri aisladas en niños chilenos con diarrea aguda

AU - Farfán U, Mauricio

AU - Flores G, Oscar

AU - Navarro G, Niurka

AU - Prado J, Valeria

AU - Mora L, Guido

AU - Toro U, Cecilia

PY - 2002/3

Y1 - 2002/3

N2 - Background: Chloramphenicol is one of the therapeutic options for shigellosis, but resistance to this antimicrobial is increasing. Aim: To characterize molecular mechanisms conferring resistance to chloramphenicol (CmR) in Shigella flexneri strains isolated from Chilean children with acute diarrhea. Material and methods: Thirty one Shigella filexneri strains, including 22 with the CmR phenotype were analyzed. Strains were tested for antimicrobial susceptibility by plate dilution and for the presence of an internal fragment of the cat gene encoding for chloramphenicol o-acetyl-transferase, by polymerase chain reaction and Southern blot analysis. Results: All CmR strains had a minimal inhibitory concentration over 64 μg/ml and amplified the internal fragment of the cat gene. Southern blot analyses indicated that this gene was located in the bacterial chromosome. Conclusions: Resistance to chloramphenicol in this group of Shigella flexneri strains was mediated by a chromosomally located cat gene.

AB - Background: Chloramphenicol is one of the therapeutic options for shigellosis, but resistance to this antimicrobial is increasing. Aim: To characterize molecular mechanisms conferring resistance to chloramphenicol (CmR) in Shigella flexneri strains isolated from Chilean children with acute diarrhea. Material and methods: Thirty one Shigella filexneri strains, including 22 with the CmR phenotype were analyzed. Strains were tested for antimicrobial susceptibility by plate dilution and for the presence of an internal fragment of the cat gene encoding for chloramphenicol o-acetyl-transferase, by polymerase chain reaction and Southern blot analysis. Results: All CmR strains had a minimal inhibitory concentration over 64 μg/ml and amplified the internal fragment of the cat gene. Southern blot analyses indicated that this gene was located in the bacterial chromosome. Conclusions: Resistance to chloramphenicol in this group of Shigella flexneri strains was mediated by a chromosomally located cat gene.

KW - Chloramphenicol o-acetyl-transferase

KW - Chloramphenicol resistance

KW - Genes, cat

KW - Shigella flexneri

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