Calpain inhibitors prevent p38 MAPK activation and germ cell apoptosis after heat stress in pubertal rat testes

Carlos Lizama, Carlos F. Lagos, Raúl Lagos-Cabré, Lorena Cantuarias, Freddy Rivera, Patricio Huenchuñir, Tomás Pérez-Acle, Flavio Carrión, Ricardo D. Moreno

Resultado de la investigación: Review article

32 Citas (Scopus)

Resumen

Testicular injuries like torsion or cryptorchidism can cause massive germ cell death, which could have great impact on male reproductive health. In addition, it has been proposed that modern life style, in the form of underwear or sedentary work position, could increase the testicular temperature, induce germ cell apoptosis, reduce spermatozoa quality and promote male infertility. In this work we showed that a heat stress stimulus induced massive germ cells apoptosis, which was associated with p38 mitogen-activated protein kinase (MAPK) phosphorylation along with an increase in the levels of mRNA encoding calpain 2. Synthetic calpain inhibitors prevented heat stress-induced germ cell apoptosis through inhibition of p38 MAPK phosphorylation. Thus, our results indicate that the blockage of calpains suppresses p38 MAPK phosphorylation, and identifies calpain activation (most likely calpain 2) as an early event in heat stress-induced male germ cell apoptosis.

Idioma originalEnglish
Páginas (desde-hasta)296-305
Número de páginas10
PublicaciónJournal of Cellular Physiology
Volumen221
N.º2
DOI
EstadoPublished - nov 2009

Huella dactilar

Calpain
p38 Mitogen-Activated Protein Kinases
Germ Cells
Phosphorylation
Testis
Rats
Hot Temperature
Chemical activation
Cells
Apoptosis
Cell death
Cryptorchidism
Torsional stress
Male Infertility
Reproductive Health
Health
Spermatozoa
Life Style
Cell Death
Messenger RNA

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Citar esto

Lizama, C., Lagos, C. F., Lagos-Cabré, R., Cantuarias, L., Rivera, F., Huenchuñir, P., ... Moreno, R. D. (2009). Calpain inhibitors prevent p38 MAPK activation and germ cell apoptosis after heat stress in pubertal rat testes. Journal of Cellular Physiology, 221(2), 296-305. https://doi.org/10.1002/jcp.21868
Lizama, Carlos ; Lagos, Carlos F. ; Lagos-Cabré, Raúl ; Cantuarias, Lorena ; Rivera, Freddy ; Huenchuñir, Patricio ; Pérez-Acle, Tomás ; Carrión, Flavio ; Moreno, Ricardo D. / Calpain inhibitors prevent p38 MAPK activation and germ cell apoptosis after heat stress in pubertal rat testes. En: Journal of Cellular Physiology. 2009 ; Vol. 221, N.º 2. pp. 296-305.
@article{75d66a8328bf476aaf0db007ee571337,
title = "Calpain inhibitors prevent p38 MAPK activation and germ cell apoptosis after heat stress in pubertal rat testes",
abstract = "Testicular injuries like torsion or cryptorchidism can cause massive germ cell death, which could have great impact on male reproductive health. In addition, it has been proposed that modern life style, in the form of underwear or sedentary work position, could increase the testicular temperature, induce germ cell apoptosis, reduce spermatozoa quality and promote male infertility. In this work we showed that a heat stress stimulus induced massive germ cells apoptosis, which was associated with p38 mitogen-activated protein kinase (MAPK) phosphorylation along with an increase in the levels of mRNA encoding calpain 2. Synthetic calpain inhibitors prevented heat stress-induced germ cell apoptosis through inhibition of p38 MAPK phosphorylation. Thus, our results indicate that the blockage of calpains suppresses p38 MAPK phosphorylation, and identifies calpain activation (most likely calpain 2) as an early event in heat stress-induced male germ cell apoptosis.",
author = "Carlos Lizama and Lagos, {Carlos F.} and Ra{\'u}l Lagos-Cabr{\'e} and Lorena Cantuarias and Freddy Rivera and Patricio Huenchu{\~n}ir and Tom{\'a}s P{\'e}rez-Acle and Flavio Carri{\'o}n and Moreno, {Ricardo D.}",
year = "2009",
month = "11",
doi = "10.1002/jcp.21868",
language = "English",
volume = "221",
pages = "296--305",
journal = "Journal of Cellular Physiology",
issn = "0021-9541",
publisher = "Wiley-Liss Inc.",
number = "2",

}

Lizama, C, Lagos, CF, Lagos-Cabré, R, Cantuarias, L, Rivera, F, Huenchuñir, P, Pérez-Acle, T, Carrión, F & Moreno, RD 2009, 'Calpain inhibitors prevent p38 MAPK activation and germ cell apoptosis after heat stress in pubertal rat testes', Journal of Cellular Physiology, vol. 221, n.º 2, pp. 296-305. https://doi.org/10.1002/jcp.21868

Calpain inhibitors prevent p38 MAPK activation and germ cell apoptosis after heat stress in pubertal rat testes. / Lizama, Carlos; Lagos, Carlos F.; Lagos-Cabré, Raúl; Cantuarias, Lorena; Rivera, Freddy; Huenchuñir, Patricio; Pérez-Acle, Tomás; Carrión, Flavio; Moreno, Ricardo D.

En: Journal of Cellular Physiology, Vol. 221, N.º 2, 11.2009, p. 296-305.

Resultado de la investigación: Review article

TY - JOUR

T1 - Calpain inhibitors prevent p38 MAPK activation and germ cell apoptosis after heat stress in pubertal rat testes

AU - Lizama, Carlos

AU - Lagos, Carlos F.

AU - Lagos-Cabré, Raúl

AU - Cantuarias, Lorena

AU - Rivera, Freddy

AU - Huenchuñir, Patricio

AU - Pérez-Acle, Tomás

AU - Carrión, Flavio

AU - Moreno, Ricardo D.

PY - 2009/11

Y1 - 2009/11

N2 - Testicular injuries like torsion or cryptorchidism can cause massive germ cell death, which could have great impact on male reproductive health. In addition, it has been proposed that modern life style, in the form of underwear or sedentary work position, could increase the testicular temperature, induce germ cell apoptosis, reduce spermatozoa quality and promote male infertility. In this work we showed that a heat stress stimulus induced massive germ cells apoptosis, which was associated with p38 mitogen-activated protein kinase (MAPK) phosphorylation along with an increase in the levels of mRNA encoding calpain 2. Synthetic calpain inhibitors prevented heat stress-induced germ cell apoptosis through inhibition of p38 MAPK phosphorylation. Thus, our results indicate that the blockage of calpains suppresses p38 MAPK phosphorylation, and identifies calpain activation (most likely calpain 2) as an early event in heat stress-induced male germ cell apoptosis.

AB - Testicular injuries like torsion or cryptorchidism can cause massive germ cell death, which could have great impact on male reproductive health. In addition, it has been proposed that modern life style, in the form of underwear or sedentary work position, could increase the testicular temperature, induce germ cell apoptosis, reduce spermatozoa quality and promote male infertility. In this work we showed that a heat stress stimulus induced massive germ cells apoptosis, which was associated with p38 mitogen-activated protein kinase (MAPK) phosphorylation along with an increase in the levels of mRNA encoding calpain 2. Synthetic calpain inhibitors prevented heat stress-induced germ cell apoptosis through inhibition of p38 MAPK phosphorylation. Thus, our results indicate that the blockage of calpains suppresses p38 MAPK phosphorylation, and identifies calpain activation (most likely calpain 2) as an early event in heat stress-induced male germ cell apoptosis.

UR - http://www.scopus.com/inward/record.url?scp=70149105546&partnerID=8YFLogxK

U2 - 10.1002/jcp.21868

DO - 10.1002/jcp.21868

M3 - Review article

C2 - 19598167

AN - SCOPUS:70149105546

VL - 221

SP - 296

EP - 305

JO - Journal of Cellular Physiology

JF - Journal of Cellular Physiology

SN - 0021-9541

IS - 2

ER -