C-di-GMP pathway in biomining bacteria

M. Castro, L. M. Ruíz, A. Barriga, C. A. Jerez, D. Holmes, N. Guiliani

Resultado de la investigación: Conference contribution

10 Citas (Scopus)

Resumen

Acidithiobacillus ferrooxidans, A. thiooxidans, and A. caldus are acidophilic Gramnegative g-proteobacteria involved in the bioleaching of metal sulfides. Bacterial attachment to mineral surface and biofilm development play a pivotal role in this process. Therefore, the understanding of biofilm formation has relevance to the design of biological strategies to improve the efficiency of bioleaching processes. For this reason, our laboratory is focused on the characterization of the molecular mechanisms involved in biofilm formation in biomining bacteria. In many bacteria, the intracellular level of c-di-GMP molecules regulates the transition from the motile planktonic state to sessile community-based behaviors, such as biofilm development. Thus, we recently started the study of c-di-GMP pathway in biomining bacteria. C-di-GMP molecules are synthesized by diguanylate cyclases (DGCs) and degraded by phosphodiesterases (PDEs). So far, two kinds of effectors have been identified, including three protein families (pilZ, PleD and FleQ) and a conserved RNA domain (GEMM) which acts as a riboswitch. We previously reported the existence of different molecular players involved in c-di-GMP pathway in A. ferrooxidans ATCC 23270. Here, we expanded our work to other Acidithioibacillus species: A. thiooxidans ATCC 19377 and A. caldus ATCC 51756. In both, we identified several putative-ORFs encoding DGC, PDE and effector proteins. By using total RNA extracted from A. ferrooxidans and A. caldus cells in RT-PCR and qPCR experiments, we demonstrated that these genes are expressed. In addition, we characterized the presence of c-di-GMP in A. ferrooxidans ATCC 23270 and A. caldus ATCC 51756 cell extracts. Taken together, these results strongly suggest that A. ferrooxidans, A. caldus and A. thiooxidans possess functional c-di-GMP pathways. As it occurs in other Gram-negative bacteria, this pathway should be involved in the regulation of the planktonic/biofilm switch. In the future, we have to integrate this new biological dimension to improve the biological understanding of bioleaching.

Idioma originalEnglish
Título de la publicación alojadaBiohydrometallurgy 2009
Páginas223-226
Número de páginas4
Volumen71-73
DOI
EstadoPublished - 2009
Evento18th International Biohydrometallurgy Symposium, IBS 2009 - Bariloche, Argentina
Duración: 13 sep 200917 sep 2009

Serie de la publicación

NombreAdvanced Materials Research
Volumen71-73
ISSN (versión impresa)10226680

Other

Other18th International Biohydrometallurgy Symposium, IBS 2009
PaísArgentina
CiudadBariloche
Período13/09/0917/09/09

Huella dactilar

Biofilms
Bacteria
Bioleaching
RNA
Proteins
Molecules
Minerals
Genes
Switches
Metals
Experiments

ASJC Scopus subject areas

  • Engineering(all)

Citar esto

Castro, M., Ruíz, L. M., Barriga, A., Jerez, C. A., Holmes, D., & Guiliani, N. (2009). C-di-GMP pathway in biomining bacteria. En Biohydrometallurgy 2009 (Vol. 71-73, pp. 223-226). (Advanced Materials Research; Vol. 71-73). https://doi.org/10.4028/www.scientific.net/AMR.71-73.223
Castro, M. ; Ruíz, L. M. ; Barriga, A. ; Jerez, C. A. ; Holmes, D. ; Guiliani, N. / C-di-GMP pathway in biomining bacteria. Biohydrometallurgy 2009. Vol. 71-73 2009. pp. 223-226 (Advanced Materials Research).
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abstract = "Acidithiobacillus ferrooxidans, A. thiooxidans, and A. caldus are acidophilic Gramnegative g-proteobacteria involved in the bioleaching of metal sulfides. Bacterial attachment to mineral surface and biofilm development play a pivotal role in this process. Therefore, the understanding of biofilm formation has relevance to the design of biological strategies to improve the efficiency of bioleaching processes. For this reason, our laboratory is focused on the characterization of the molecular mechanisms involved in biofilm formation in biomining bacteria. In many bacteria, the intracellular level of c-di-GMP molecules regulates the transition from the motile planktonic state to sessile community-based behaviors, such as biofilm development. Thus, we recently started the study of c-di-GMP pathway in biomining bacteria. C-di-GMP molecules are synthesized by diguanylate cyclases (DGCs) and degraded by phosphodiesterases (PDEs). So far, two kinds of effectors have been identified, including three protein families (pilZ, PleD and FleQ) and a conserved RNA domain (GEMM) which acts as a riboswitch. We previously reported the existence of different molecular players involved in c-di-GMP pathway in A. ferrooxidans ATCC 23270. Here, we expanded our work to other Acidithioibacillus species: A. thiooxidans ATCC 19377 and A. caldus ATCC 51756. In both, we identified several putative-ORFs encoding DGC, PDE and effector proteins. By using total RNA extracted from A. ferrooxidans and A. caldus cells in RT-PCR and qPCR experiments, we demonstrated that these genes are expressed. In addition, we characterized the presence of c-di-GMP in A. ferrooxidans ATCC 23270 and A. caldus ATCC 51756 cell extracts. Taken together, these results strongly suggest that A. ferrooxidans, A. caldus and A. thiooxidans possess functional c-di-GMP pathways. As it occurs in other Gram-negative bacteria, this pathway should be involved in the regulation of the planktonic/biofilm switch. In the future, we have to integrate this new biological dimension to improve the biological understanding of bioleaching.",
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Castro, M, Ruíz, LM, Barriga, A, Jerez, CA, Holmes, D & Guiliani, N 2009, C-di-GMP pathway in biomining bacteria. En Biohydrometallurgy 2009. vol. 71-73, Advanced Materials Research, vol. 71-73, pp. 223-226, 18th International Biohydrometallurgy Symposium, IBS 2009, Bariloche, Argentina, 13/09/09. https://doi.org/10.4028/www.scientific.net/AMR.71-73.223

C-di-GMP pathway in biomining bacteria. / Castro, M.; Ruíz, L. M.; Barriga, A.; Jerez, C. A.; Holmes, D.; Guiliani, N.

Biohydrometallurgy 2009. Vol. 71-73 2009. p. 223-226 (Advanced Materials Research; Vol. 71-73).

Resultado de la investigación: Conference contribution

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T1 - C-di-GMP pathway in biomining bacteria

AU - Castro, M.

AU - Ruíz, L. M.

AU - Barriga, A.

AU - Jerez, C. A.

AU - Holmes, D.

AU - Guiliani, N.

PY - 2009

Y1 - 2009

N2 - Acidithiobacillus ferrooxidans, A. thiooxidans, and A. caldus are acidophilic Gramnegative g-proteobacteria involved in the bioleaching of metal sulfides. Bacterial attachment to mineral surface and biofilm development play a pivotal role in this process. Therefore, the understanding of biofilm formation has relevance to the design of biological strategies to improve the efficiency of bioleaching processes. For this reason, our laboratory is focused on the characterization of the molecular mechanisms involved in biofilm formation in biomining bacteria. In many bacteria, the intracellular level of c-di-GMP molecules regulates the transition from the motile planktonic state to sessile community-based behaviors, such as biofilm development. Thus, we recently started the study of c-di-GMP pathway in biomining bacteria. C-di-GMP molecules are synthesized by diguanylate cyclases (DGCs) and degraded by phosphodiesterases (PDEs). So far, two kinds of effectors have been identified, including three protein families (pilZ, PleD and FleQ) and a conserved RNA domain (GEMM) which acts as a riboswitch. We previously reported the existence of different molecular players involved in c-di-GMP pathway in A. ferrooxidans ATCC 23270. Here, we expanded our work to other Acidithioibacillus species: A. thiooxidans ATCC 19377 and A. caldus ATCC 51756. In both, we identified several putative-ORFs encoding DGC, PDE and effector proteins. By using total RNA extracted from A. ferrooxidans and A. caldus cells in RT-PCR and qPCR experiments, we demonstrated that these genes are expressed. In addition, we characterized the presence of c-di-GMP in A. ferrooxidans ATCC 23270 and A. caldus ATCC 51756 cell extracts. Taken together, these results strongly suggest that A. ferrooxidans, A. caldus and A. thiooxidans possess functional c-di-GMP pathways. As it occurs in other Gram-negative bacteria, this pathway should be involved in the regulation of the planktonic/biofilm switch. In the future, we have to integrate this new biological dimension to improve the biological understanding of bioleaching.

AB - Acidithiobacillus ferrooxidans, A. thiooxidans, and A. caldus are acidophilic Gramnegative g-proteobacteria involved in the bioleaching of metal sulfides. Bacterial attachment to mineral surface and biofilm development play a pivotal role in this process. Therefore, the understanding of biofilm formation has relevance to the design of biological strategies to improve the efficiency of bioleaching processes. For this reason, our laboratory is focused on the characterization of the molecular mechanisms involved in biofilm formation in biomining bacteria. In many bacteria, the intracellular level of c-di-GMP molecules regulates the transition from the motile planktonic state to sessile community-based behaviors, such as biofilm development. Thus, we recently started the study of c-di-GMP pathway in biomining bacteria. C-di-GMP molecules are synthesized by diguanylate cyclases (DGCs) and degraded by phosphodiesterases (PDEs). So far, two kinds of effectors have been identified, including three protein families (pilZ, PleD and FleQ) and a conserved RNA domain (GEMM) which acts as a riboswitch. We previously reported the existence of different molecular players involved in c-di-GMP pathway in A. ferrooxidans ATCC 23270. Here, we expanded our work to other Acidithioibacillus species: A. thiooxidans ATCC 19377 and A. caldus ATCC 51756. In both, we identified several putative-ORFs encoding DGC, PDE and effector proteins. By using total RNA extracted from A. ferrooxidans and A. caldus cells in RT-PCR and qPCR experiments, we demonstrated that these genes are expressed. In addition, we characterized the presence of c-di-GMP in A. ferrooxidans ATCC 23270 and A. caldus ATCC 51756 cell extracts. Taken together, these results strongly suggest that A. ferrooxidans, A. caldus and A. thiooxidans possess functional c-di-GMP pathways. As it occurs in other Gram-negative bacteria, this pathway should be involved in the regulation of the planktonic/biofilm switch. In the future, we have to integrate this new biological dimension to improve the biological understanding of bioleaching.

KW - Acidithiobacillus

KW - Biofilm

KW - Biomining

KW - c-di-GMP

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M3 - Conference contribution

AN - SCOPUS:72449174237

SN - 0878493220

SN - 9780878493227

VL - 71-73

T3 - Advanced Materials Research

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BT - Biohydrometallurgy 2009

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Castro M, Ruíz LM, Barriga A, Jerez CA, Holmes D, Guiliani N. C-di-GMP pathway in biomining bacteria. En Biohydrometallurgy 2009. Vol. 71-73. 2009. p. 223-226. (Advanced Materials Research). https://doi.org/10.4028/www.scientific.net/AMR.71-73.223