Biosynthesis of cds quantum dots mediated by volatile sulfur compounds released by antarctic pseudomonas fragi

Carla Gallardo-Benavente, Ornella Carrión, Jonathan D. Todd, Joana C. Pieretti, Amedea B. Seabra, Nelson Durán, Olga Rubilar, José M. Pérez-Donoso, Andrés Quiroz

Resultado de la investigación: Article

Resumen

Previously we reported the biosynthesis of intracellular cadmium sulfide quantum dots (CdS QDs) at low temperatures by the Antarctic strain Pseudomonas fragi GC01. Here we studied the role of volatile sulfur compounds (VSCs) in the biosynthesis of CdS QDs by P. fragi GC01. The biosynthesis of nanoparticles was evaluated in the presence of sulfate, sulfite, thiosulfate, sulfide, cysteine and methionine as sole sulfur sources. Intracellular biosynthesis occurred with all sulfur sources tested. However, extracellular biosynthesis was observed only in cultures amended with cysteine (Cys) and methionine (Met). Extracellular nanoparticles were characterized by dynamic light scattering, absorption and emission spectra, energy dispersive X-ray, atomic force microscopy, transmission electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. Purified QDs correspond to cubic nanocrystals of CdS with sizes between 2 and 16 nm. The analysis of VSCs revealed that P. fragi GC01 produced hydrogen sulfide (H2S), methanethiol (MeSH) and dimethyl sulfide (DMS) in the presence of sulfate, Met or Cys. Dimethyl disulfide (DMDS) was only detected in the presence of Met. Interestingly, MeSH was the main VSC produced in this condition. In addition, MeSH was the only VSC for which the concentration decreased in the presence of cadmium (Cd) of all the sulfur sources tested, suggesting that this gas interacts with Cd to form nanoparticles. The role of MeSH and DMS on Cds QDs biosynthesis was evaluated in two mutants of the Antarctic strain Pseudomonas deceptionensis M1T: megL (unable to produce MeSH from Met) and mddA (unable to generate DMS from MeSH). No biosynthesis of QDs was observed in the megL strain, confirming the importance of MeSH in QD biosynthesis. In addition, the production of QDs in the mddA strain was not affected, indicating that DMS is not a substrate for the biosynthesis of nanoparticles. Here, we confirm a link between MeSH production and CdS QDs biosynthesis when Met is used as sole sulfur source. This work represents the first report that directly associates the production of MeSH with the bacterial synthesis of QDs, thus revealing the importance of different VSCs in the biological generation of metal sulfide nanostructures.

Idioma originalEnglish
Número de artículo1866
PublicaciónFrontiers in Microbiology
Volumen10
N.ºAUG
DOI
EstadoPublished - 13 ago 2019

Huella dactilar

Pseudomonas fragi
Sulfur Compounds
Quantum Dots
Methionine
Nanoparticles
Sulfur
Cysteine
Sulfides
Cadmium
Sulfates
Thiosulfates
Photoelectron Spectroscopy
Hydrogen Sulfide
Sulfites
Nanostructures
Atomic Force Microscopy
Pseudomonas
Transmission Electron Microscopy
X-Ray Diffraction
Gases

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Citar esto

Gallardo-Benavente, C., Carrión, O., Todd, J. D., Pieretti, J. C., Seabra, A. B., Durán, N., ... Quiroz, A. (2019). Biosynthesis of cds quantum dots mediated by volatile sulfur compounds released by antarctic pseudomonas fragi. Frontiers in Microbiology, 10(AUG), [1866]. https://doi.org/10.3389/fmicb.2019.01866
Gallardo-Benavente, Carla ; Carrión, Ornella ; Todd, Jonathan D. ; Pieretti, Joana C. ; Seabra, Amedea B. ; Durán, Nelson ; Rubilar, Olga ; Pérez-Donoso, José M. ; Quiroz, Andrés. / Biosynthesis of cds quantum dots mediated by volatile sulfur compounds released by antarctic pseudomonas fragi. En: Frontiers in Microbiology. 2019 ; Vol. 10, N.º AUG.
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abstract = "Previously we reported the biosynthesis of intracellular cadmium sulfide quantum dots (CdS QDs) at low temperatures by the Antarctic strain Pseudomonas fragi GC01. Here we studied the role of volatile sulfur compounds (VSCs) in the biosynthesis of CdS QDs by P. fragi GC01. The biosynthesis of nanoparticles was evaluated in the presence of sulfate, sulfite, thiosulfate, sulfide, cysteine and methionine as sole sulfur sources. Intracellular biosynthesis occurred with all sulfur sources tested. However, extracellular biosynthesis was observed only in cultures amended with cysteine (Cys) and methionine (Met). Extracellular nanoparticles were characterized by dynamic light scattering, absorption and emission spectra, energy dispersive X-ray, atomic force microscopy, transmission electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. Purified QDs correspond to cubic nanocrystals of CdS with sizes between 2 and 16 nm. The analysis of VSCs revealed that P. fragi GC01 produced hydrogen sulfide (H2S), methanethiol (MeSH) and dimethyl sulfide (DMS) in the presence of sulfate, Met or Cys. Dimethyl disulfide (DMDS) was only detected in the presence of Met. Interestingly, MeSH was the main VSC produced in this condition. In addition, MeSH was the only VSC for which the concentration decreased in the presence of cadmium (Cd) of all the sulfur sources tested, suggesting that this gas interacts with Cd to form nanoparticles. The role of MeSH and DMS on Cds QDs biosynthesis was evaluated in two mutants of the Antarctic strain Pseudomonas deceptionensis M1T: megL− (unable to produce MeSH from Met) and mddA− (unable to generate DMS from MeSH). No biosynthesis of QDs was observed in the megL− strain, confirming the importance of MeSH in QD biosynthesis. In addition, the production of QDs in the mddA− strain was not affected, indicating that DMS is not a substrate for the biosynthesis of nanoparticles. Here, we confirm a link between MeSH production and CdS QDs biosynthesis when Met is used as sole sulfur source. This work represents the first report that directly associates the production of MeSH with the bacterial synthesis of QDs, thus revealing the importance of different VSCs in the biological generation of metal sulfide nanostructures.",
keywords = "Antarctic bacteria, Cadmium sulfide, Nanoparticle biosynthesis, Quantum dot, Volatile sulfur compounds",
author = "Carla Gallardo-Benavente and Ornella Carri{\'o}n and Todd, {Jonathan D.} and Pieretti, {Joana C.} and Seabra, {Amedea B.} and Nelson Dur{\'a}n and Olga Rubilar and P{\'e}rez-Donoso, {Jos{\'e} M.} and Andr{\'e}s Quiroz",
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Gallardo-Benavente, C, Carrión, O, Todd, JD, Pieretti, JC, Seabra, AB, Durán, N, Rubilar, O, Pérez-Donoso, JM & Quiroz, A 2019, 'Biosynthesis of cds quantum dots mediated by volatile sulfur compounds released by antarctic pseudomonas fragi', Frontiers in Microbiology, vol. 10, n.º AUG, 1866. https://doi.org/10.3389/fmicb.2019.01866

Biosynthesis of cds quantum dots mediated by volatile sulfur compounds released by antarctic pseudomonas fragi. / Gallardo-Benavente, Carla; Carrión, Ornella; Todd, Jonathan D.; Pieretti, Joana C.; Seabra, Amedea B.; Durán, Nelson; Rubilar, Olga; Pérez-Donoso, José M.; Quiroz, Andrés.

En: Frontiers in Microbiology, Vol. 10, N.º AUG, 1866, 13.08.2019.

Resultado de la investigación: Article

TY - JOUR

T1 - Biosynthesis of cds quantum dots mediated by volatile sulfur compounds released by antarctic pseudomonas fragi

AU - Gallardo-Benavente, Carla

AU - Carrión, Ornella

AU - Todd, Jonathan D.

AU - Pieretti, Joana C.

AU - Seabra, Amedea B.

AU - Durán, Nelson

AU - Rubilar, Olga

AU - Pérez-Donoso, José M.

AU - Quiroz, Andrés

PY - 2019/8/13

Y1 - 2019/8/13

N2 - Previously we reported the biosynthesis of intracellular cadmium sulfide quantum dots (CdS QDs) at low temperatures by the Antarctic strain Pseudomonas fragi GC01. Here we studied the role of volatile sulfur compounds (VSCs) in the biosynthesis of CdS QDs by P. fragi GC01. The biosynthesis of nanoparticles was evaluated in the presence of sulfate, sulfite, thiosulfate, sulfide, cysteine and methionine as sole sulfur sources. Intracellular biosynthesis occurred with all sulfur sources tested. However, extracellular biosynthesis was observed only in cultures amended with cysteine (Cys) and methionine (Met). Extracellular nanoparticles were characterized by dynamic light scattering, absorption and emission spectra, energy dispersive X-ray, atomic force microscopy, transmission electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. Purified QDs correspond to cubic nanocrystals of CdS with sizes between 2 and 16 nm. The analysis of VSCs revealed that P. fragi GC01 produced hydrogen sulfide (H2S), methanethiol (MeSH) and dimethyl sulfide (DMS) in the presence of sulfate, Met or Cys. Dimethyl disulfide (DMDS) was only detected in the presence of Met. Interestingly, MeSH was the main VSC produced in this condition. In addition, MeSH was the only VSC for which the concentration decreased in the presence of cadmium (Cd) of all the sulfur sources tested, suggesting that this gas interacts with Cd to form nanoparticles. The role of MeSH and DMS on Cds QDs biosynthesis was evaluated in two mutants of the Antarctic strain Pseudomonas deceptionensis M1T: megL− (unable to produce MeSH from Met) and mddA− (unable to generate DMS from MeSH). No biosynthesis of QDs was observed in the megL− strain, confirming the importance of MeSH in QD biosynthesis. In addition, the production of QDs in the mddA− strain was not affected, indicating that DMS is not a substrate for the biosynthesis of nanoparticles. Here, we confirm a link between MeSH production and CdS QDs biosynthesis when Met is used as sole sulfur source. This work represents the first report that directly associates the production of MeSH with the bacterial synthesis of QDs, thus revealing the importance of different VSCs in the biological generation of metal sulfide nanostructures.

AB - Previously we reported the biosynthesis of intracellular cadmium sulfide quantum dots (CdS QDs) at low temperatures by the Antarctic strain Pseudomonas fragi GC01. Here we studied the role of volatile sulfur compounds (VSCs) in the biosynthesis of CdS QDs by P. fragi GC01. The biosynthesis of nanoparticles was evaluated in the presence of sulfate, sulfite, thiosulfate, sulfide, cysteine and methionine as sole sulfur sources. Intracellular biosynthesis occurred with all sulfur sources tested. However, extracellular biosynthesis was observed only in cultures amended with cysteine (Cys) and methionine (Met). Extracellular nanoparticles were characterized by dynamic light scattering, absorption and emission spectra, energy dispersive X-ray, atomic force microscopy, transmission electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. Purified QDs correspond to cubic nanocrystals of CdS with sizes between 2 and 16 nm. The analysis of VSCs revealed that P. fragi GC01 produced hydrogen sulfide (H2S), methanethiol (MeSH) and dimethyl sulfide (DMS) in the presence of sulfate, Met or Cys. Dimethyl disulfide (DMDS) was only detected in the presence of Met. Interestingly, MeSH was the main VSC produced in this condition. In addition, MeSH was the only VSC for which the concentration decreased in the presence of cadmium (Cd) of all the sulfur sources tested, suggesting that this gas interacts with Cd to form nanoparticles. The role of MeSH and DMS on Cds QDs biosynthesis was evaluated in two mutants of the Antarctic strain Pseudomonas deceptionensis M1T: megL− (unable to produce MeSH from Met) and mddA− (unable to generate DMS from MeSH). No biosynthesis of QDs was observed in the megL− strain, confirming the importance of MeSH in QD biosynthesis. In addition, the production of QDs in the mddA− strain was not affected, indicating that DMS is not a substrate for the biosynthesis of nanoparticles. Here, we confirm a link between MeSH production and CdS QDs biosynthesis when Met is used as sole sulfur source. This work represents the first report that directly associates the production of MeSH with the bacterial synthesis of QDs, thus revealing the importance of different VSCs in the biological generation of metal sulfide nanostructures.

KW - Antarctic bacteria

KW - Cadmium sulfide

KW - Nanoparticle biosynthesis

KW - Quantum dot

KW - Volatile sulfur compounds

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U2 - 10.3389/fmicb.2019.01866

DO - 10.3389/fmicb.2019.01866

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JO - Frontiers in Microbiology

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