Biological activity and chemical characterization of Pouteria lucuma seeds: A possible use of an agricultural waste

Pedro Guerrero-Castillo, Sarita Reyes, Juana Robles, Mario J. Simirgiotis, Beatriz Sepulveda, Ronald Fernandez-Burgos, Carlos Areche

Resultado de la investigación: Article

Resumen

Pouteria lucuma fruit is widely used to prepare cakes, ice creams and juice or also commercialized as pulp and flour. As result of this business thousands of tons of seeds are generated as an agricultural waste. This study presents the antioxidant and antiulcer activities, and the identification of secondary metabolites by UHPLC/ESI/MS/MS of an agroindustrial waste of Pouteria lucuma seeds. Fifty-nine compounds were tentatively identified including eight aminoacids, five organic acids, one nucleoside, five phenolic acids, five phenolic alcohols, nineteen flavonoids, six lipids, and seven unknowns in the methanol extract of P. lucuma seeds. The total phenolic content of the seeds was 52.82 ± 0.09 μmol GAE/g dry weight, while total flavonoid content was 5.99 ± 0.01 μmol Q/g dry weight. The antioxidant activity was 58.14 ± 0.05, 66.97 ± 0.00, 272.50 ± 0.00, and 67.02 ± 2.23 for the DPPH, ABTS, FRAP, and superoxide anion assays, respectively. The highest gastroprotective activity was obtained at 100 mg/kg (78%), which as higher than the positive control lansoprazole (75%). Our findings showed that P. lucuma seed extracts have moderate to high antioxidant activity and gastroprotective properties. Therefore, it was demostrated that lucuma seeds commonly eliminated as an agricultural industry waste, could be useful for the preparation of nutritional supplements.

Idioma originalEnglish
Páginas (desde-hasta)319-327
Número de páginas9
PublicaciónWaste Management
Volumen88
DOI
EstadoPublished - 1 abr 2019

Huella dactilar

seed
antioxidant
secondary metabolite
organic acid
methanol
chemical
agricultural waste
biological activity
anion
alcohol
fruit
lipid
assay
ice
industry
acid

ASJC Scopus subject areas

  • Waste Management and Disposal

Citar esto

Guerrero-Castillo, P., Reyes, S., Robles, J., Simirgiotis, M. J., Sepulveda, B., Fernandez-Burgos, R., & Areche, C. (2019). Biological activity and chemical characterization of Pouteria lucuma seeds: A possible use of an agricultural waste. Waste Management, 88, 319-327. https://doi.org/10.1016/j.wasman.2019.03.055
Guerrero-Castillo, Pedro ; Reyes, Sarita ; Robles, Juana ; Simirgiotis, Mario J. ; Sepulveda, Beatriz ; Fernandez-Burgos, Ronald ; Areche, Carlos. / Biological activity and chemical characterization of Pouteria lucuma seeds : A possible use of an agricultural waste. En: Waste Management. 2019 ; Vol. 88. pp. 319-327.
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Guerrero-Castillo, P, Reyes, S, Robles, J, Simirgiotis, MJ, Sepulveda, B, Fernandez-Burgos, R & Areche, C 2019, 'Biological activity and chemical characterization of Pouteria lucuma seeds: A possible use of an agricultural waste', Waste Management, vol. 88, pp. 319-327. https://doi.org/10.1016/j.wasman.2019.03.055

Biological activity and chemical characterization of Pouteria lucuma seeds : A possible use of an agricultural waste. / Guerrero-Castillo, Pedro; Reyes, Sarita; Robles, Juana; Simirgiotis, Mario J.; Sepulveda, Beatriz; Fernandez-Burgos, Ronald; Areche, Carlos.

En: Waste Management, Vol. 88, 01.04.2019, p. 319-327.

Resultado de la investigación: Article

TY - JOUR

T1 - Biological activity and chemical characterization of Pouteria lucuma seeds

T2 - A possible use of an agricultural waste

AU - Guerrero-Castillo, Pedro

AU - Reyes, Sarita

AU - Robles, Juana

AU - Simirgiotis, Mario J.

AU - Sepulveda, Beatriz

AU - Fernandez-Burgos, Ronald

AU - Areche, Carlos

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Pouteria lucuma fruit is widely used to prepare cakes, ice creams and juice or also commercialized as pulp and flour. As result of this business thousands of tons of seeds are generated as an agricultural waste. This study presents the antioxidant and antiulcer activities, and the identification of secondary metabolites by UHPLC/ESI/MS/MS of an agroindustrial waste of Pouteria lucuma seeds. Fifty-nine compounds were tentatively identified including eight aminoacids, five organic acids, one nucleoside, five phenolic acids, five phenolic alcohols, nineteen flavonoids, six lipids, and seven unknowns in the methanol extract of P. lucuma seeds. The total phenolic content of the seeds was 52.82 ± 0.09 μmol GAE/g dry weight, while total flavonoid content was 5.99 ± 0.01 μmol Q/g dry weight. The antioxidant activity was 58.14 ± 0.05, 66.97 ± 0.00, 272.50 ± 0.00, and 67.02 ± 2.23 for the DPPH, ABTS, FRAP, and superoxide anion assays, respectively. The highest gastroprotective activity was obtained at 100 mg/kg (78%), which as higher than the positive control lansoprazole (75%). Our findings showed that P. lucuma seed extracts have moderate to high antioxidant activity and gastroprotective properties. Therefore, it was demostrated that lucuma seeds commonly eliminated as an agricultural industry waste, could be useful for the preparation of nutritional supplements.

AB - Pouteria lucuma fruit is widely used to prepare cakes, ice creams and juice or also commercialized as pulp and flour. As result of this business thousands of tons of seeds are generated as an agricultural waste. This study presents the antioxidant and antiulcer activities, and the identification of secondary metabolites by UHPLC/ESI/MS/MS of an agroindustrial waste of Pouteria lucuma seeds. Fifty-nine compounds were tentatively identified including eight aminoacids, five organic acids, one nucleoside, five phenolic acids, five phenolic alcohols, nineteen flavonoids, six lipids, and seven unknowns in the methanol extract of P. lucuma seeds. The total phenolic content of the seeds was 52.82 ± 0.09 μmol GAE/g dry weight, while total flavonoid content was 5.99 ± 0.01 μmol Q/g dry weight. The antioxidant activity was 58.14 ± 0.05, 66.97 ± 0.00, 272.50 ± 0.00, and 67.02 ± 2.23 for the DPPH, ABTS, FRAP, and superoxide anion assays, respectively. The highest gastroprotective activity was obtained at 100 mg/kg (78%), which as higher than the positive control lansoprazole (75%). Our findings showed that P. lucuma seed extracts have moderate to high antioxidant activity and gastroprotective properties. Therefore, it was demostrated that lucuma seeds commonly eliminated as an agricultural industry waste, could be useful for the preparation of nutritional supplements.

KW - Agroindustrial waste

KW - Flavonoids

KW - LC-MS/MS

KW - Lucuma

KW - Phenolics

KW - Pouteria

KW - UHPLC-Q-exactive focus

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EP - 327

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JF - Waste Management

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Guerrero-Castillo P, Reyes S, Robles J, Simirgiotis MJ, Sepulveda B, Fernandez-Burgos R y otros. Biological activity and chemical characterization of Pouteria lucuma seeds: A possible use of an agricultural waste. Waste Management. 2019 abr 1;88:319-327. https://doi.org/10.1016/j.wasman.2019.03.055