Biofilm development and cell viability: An undervalued mechanism in the persistence of the fish pathogen Tenacibaculum maritimum

Héctor A. Levipan, D. Tapia-Cammas, Verónica Molina, Rute Irgang, Alicia E. Toranzo, Beatriz Magariños, R. Avendaño-Herrera

Resultado de la investigación: Article

1 Cita (Scopus)

Resumen

Tenacibaculum maritimum is a bacterial pathogen that causes tenacibaculosis outbreaks in major commercial marine fish worldwide. However, scarce knowledge exists regarding T. maritimum life strategies that could act as potential mechanisms for environmental persistence and transmission, such as surface-attached biofilms. This study used high-throughput analyses to quantitatively and qualitatively characterize biofilm formation and activity in T. maritimum strains ACC50.1, PC424.1, PC503.1, and CECT 4276. Additionally, cell-surface properties of the T. maritimum strains, such as hydrophobicity, were determined before inducing biofilm formation. We also briefly examined if pathogenicity against fish varied among T. maritimum strains with differing cell-surface properties. All strains were moderately hydrophobic and rapidly formed biofilms on polystyrene surfaces in a three-step process, with live and dead cells in multilayered-like bacterial aggregates. Virulence in turbot (Scophthalmus maximus) was assessed through a prolonged-immersion challenge. Turbot exhibited the classical signs of tenacibaculosis and mortalities for the T. maritimum strains PC424.1, CECT 4276, and ACC50.1, but no symptoms or mortalities were recorded against PC503.1 or non-challenged controls. Interestingly, while all T. maritimum strains tended to develop profuse biofilms over time, all biofilms showed a decreasing tendency in cell viability. (i.e., a range of fluorescent ratios of live-to-dead sessile cells of ca. 17–51 at 24 h versus 12–22 at 96 h). The fast kinetics of T. maritimum biofilm formation suggests that aquaculture settings can harbor these cellular accretions as transient reservoirs for virulent strains, possibly contributing to tenacibaculosis prevalence to a degree thus far unknown.

Idioma originalEnglish
Número de artículo734267
PublicaciónAquaculture
Volumen511
DOI
EstadoPublished - 15 sep 2019

Huella dactilar

Tenacibaculum maritimum
biofilm
cell viability
viability
persistence
pathogen
pathogens
fish
turbot
mortality
cells
pathogenicity
hydrophobicity
Scophthalmus maximus
virulence
polystyrenes
cell aggregates
aquaculture
marine fish
harbor

ASJC Scopus subject areas

  • Aquatic Science

Citar esto

Levipan, Héctor A. ; Tapia-Cammas, D. ; Molina, Verónica ; Irgang, Rute ; Toranzo, Alicia E. ; Magariños, Beatriz ; Avendaño-Herrera, R. / Biofilm development and cell viability : An undervalued mechanism in the persistence of the fish pathogen Tenacibaculum maritimum. En: Aquaculture. 2019 ; Vol. 511.
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abstract = "Tenacibaculum maritimum is a bacterial pathogen that causes tenacibaculosis outbreaks in major commercial marine fish worldwide. However, scarce knowledge exists regarding T. maritimum life strategies that could act as potential mechanisms for environmental persistence and transmission, such as surface-attached biofilms. This study used high-throughput analyses to quantitatively and qualitatively characterize biofilm formation and activity in T. maritimum strains ACC50.1, PC424.1, PC503.1, and CECT 4276. Additionally, cell-surface properties of the T. maritimum strains, such as hydrophobicity, were determined before inducing biofilm formation. We also briefly examined if pathogenicity against fish varied among T. maritimum strains with differing cell-surface properties. All strains were moderately hydrophobic and rapidly formed biofilms on polystyrene surfaces in a three-step process, with live and dead cells in multilayered-like bacterial aggregates. Virulence in turbot (Scophthalmus maximus) was assessed through a prolonged-immersion challenge. Turbot exhibited the classical signs of tenacibaculosis and mortalities for the T. maritimum strains PC424.1, CECT 4276, and ACC50.1, but no symptoms or mortalities were recorded against PC503.1 or non-challenged controls. Interestingly, while all T. maritimum strains tended to develop profuse biofilms over time, all biofilms showed a decreasing tendency in cell viability. (i.e., a range of fluorescent ratios of live-to-dead sessile cells of ca. 17–51 at 24 h versus 12–22 at 96 h). The fast kinetics of T. maritimum biofilm formation suggests that aquaculture settings can harbor these cellular accretions as transient reservoirs for virulent strains, possibly contributing to tenacibaculosis prevalence to a degree thus far unknown.",
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Biofilm development and cell viability : An undervalued mechanism in the persistence of the fish pathogen Tenacibaculum maritimum. / Levipan, Héctor A.; Tapia-Cammas, D.; Molina, Verónica; Irgang, Rute; Toranzo, Alicia E.; Magariños, Beatriz; Avendaño-Herrera, R.

En: Aquaculture, Vol. 511, 734267, 15.09.2019.

Resultado de la investigación: Article

TY - JOUR

T1 - Biofilm development and cell viability

T2 - An undervalued mechanism in the persistence of the fish pathogen Tenacibaculum maritimum

AU - Levipan, Héctor A.

AU - Tapia-Cammas, D.

AU - Molina, Verónica

AU - Irgang, Rute

AU - Toranzo, Alicia E.

AU - Magariños, Beatriz

AU - Avendaño-Herrera, R.

PY - 2019/9/15

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N2 - Tenacibaculum maritimum is a bacterial pathogen that causes tenacibaculosis outbreaks in major commercial marine fish worldwide. However, scarce knowledge exists regarding T. maritimum life strategies that could act as potential mechanisms for environmental persistence and transmission, such as surface-attached biofilms. This study used high-throughput analyses to quantitatively and qualitatively characterize biofilm formation and activity in T. maritimum strains ACC50.1, PC424.1, PC503.1, and CECT 4276. Additionally, cell-surface properties of the T. maritimum strains, such as hydrophobicity, were determined before inducing biofilm formation. We also briefly examined if pathogenicity against fish varied among T. maritimum strains with differing cell-surface properties. All strains were moderately hydrophobic and rapidly formed biofilms on polystyrene surfaces in a three-step process, with live and dead cells in multilayered-like bacterial aggregates. Virulence in turbot (Scophthalmus maximus) was assessed through a prolonged-immersion challenge. Turbot exhibited the classical signs of tenacibaculosis and mortalities for the T. maritimum strains PC424.1, CECT 4276, and ACC50.1, but no symptoms or mortalities were recorded against PC503.1 or non-challenged controls. Interestingly, while all T. maritimum strains tended to develop profuse biofilms over time, all biofilms showed a decreasing tendency in cell viability. (i.e., a range of fluorescent ratios of live-to-dead sessile cells of ca. 17–51 at 24 h versus 12–22 at 96 h). The fast kinetics of T. maritimum biofilm formation suggests that aquaculture settings can harbor these cellular accretions as transient reservoirs for virulent strains, possibly contributing to tenacibaculosis prevalence to a degree thus far unknown.

AB - Tenacibaculum maritimum is a bacterial pathogen that causes tenacibaculosis outbreaks in major commercial marine fish worldwide. However, scarce knowledge exists regarding T. maritimum life strategies that could act as potential mechanisms for environmental persistence and transmission, such as surface-attached biofilms. This study used high-throughput analyses to quantitatively and qualitatively characterize biofilm formation and activity in T. maritimum strains ACC50.1, PC424.1, PC503.1, and CECT 4276. Additionally, cell-surface properties of the T. maritimum strains, such as hydrophobicity, were determined before inducing biofilm formation. We also briefly examined if pathogenicity against fish varied among T. maritimum strains with differing cell-surface properties. All strains were moderately hydrophobic and rapidly formed biofilms on polystyrene surfaces in a three-step process, with live and dead cells in multilayered-like bacterial aggregates. Virulence in turbot (Scophthalmus maximus) was assessed through a prolonged-immersion challenge. Turbot exhibited the classical signs of tenacibaculosis and mortalities for the T. maritimum strains PC424.1, CECT 4276, and ACC50.1, but no symptoms or mortalities were recorded against PC503.1 or non-challenged controls. Interestingly, while all T. maritimum strains tended to develop profuse biofilms over time, all biofilms showed a decreasing tendency in cell viability. (i.e., a range of fluorescent ratios of live-to-dead sessile cells of ca. 17–51 at 24 h versus 12–22 at 96 h). The fast kinetics of T. maritimum biofilm formation suggests that aquaculture settings can harbor these cellular accretions as transient reservoirs for virulent strains, possibly contributing to tenacibaculosis prevalence to a degree thus far unknown.

KW - Biofilm phenotype

KW - Fish pathogen

KW - Live/dead ratio

KW - Sessile bacteria

KW - Tenacibaculosis

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