TY - JOUR
T1 - Biochemistry, genetics and biotechnology of glycerol utilization in pseudomonas species
AU - Poblete-Castro, Ignacio
AU - Wittmann, Christoph
AU - Nikel, Pablo I.
N1 - Funding Information:
This study was supported by The Novo Nordisk Foundation (Grant NNF10CC1016517) and the Danish Council for Independent Research (, DFF‐Research Project 8021‐00039B) to P.I.N. This work was also supported by CONICYT through the project Fondecyt Inicio 11150174. SWEET
Funding Information:
Funding Information This study was supported by The Novo Nordisk Foundation (Grant NNF10CC1016517) and the Danish Council for Independent Research (SWEET, DFF-Research Project 8021-00039B) to P.I.N. This work was also supported by CONICYT through the project Fondecyt Inicio 11150174. The authors are indebted to Prof. V. de Lorenzo (CNB-CSIC, Madrid) for inspiring discussions and critical reading of the manuscript. This study was supported by The Novo Nordisk Foundation (Grant NNF10CC1016517) and the Danish Council for Independent Research (SWEET, DFF-Research Project 8021-00039B) to P.I.N. This work was also supported by CONICYT through the project Fondecyt Inicio 11150174 to I.P.C. C.W. acknowledges support by the German Ministry for Education and Research (LignoValue, Project 01DN17036). The funding bodies were not involved in the design, collection, analysis or interpretation of this study.
Funding Information:
The authors are indebted to Prof. V. de Lorenzo (CNB‐CSIC, Madrid) for inspiring discussions and critical reading of the manuscript. This study was supported by The Novo Nordisk Foundation (Grant NNF10CC1016517) and the Danish Council for Independent Research (, DFF‐Research Project 8021‐00039B) to P.I.N. This work was also supported by CONICYT through the project Fondecyt Inicio 11150174 to I.P.C. C.W. acknowledges support by the German Ministry for Education and Research (, Project 01DN17036). The funding bodies were not involved in the design, collection, analysis or interpretation of this study. SWEET LignoValue
Publisher Copyright:
© 2019 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.
PY - 2020/1/1
Y1 - 2020/1/1
N2 - The use of renewable waste feedstocks is an environ-ment-friendly choice contributing to the reduction of waste treatment costs and increasing the economic value of industrial by-products. Glycerol (1,2,3-propa-netriol), a simple polyol compound widely distributed in biological systems, constitutes a prime example of a relatively cheap and readily available substrate to be used in bioprocesses. Extensively exploited as an ingredient in the food and pharmaceutical industries, glycerol is also the main by-product of biodiesel production, which has resulted in a progressive drop in substrate price over the years. Consequently, glycerol has become an attractive substrate in biotechnology, and several chemical commodities currently produced from petroleum have been shown to be obtained from this polyol using whole-cell biocata-lysts with both wild-type and engineered bacterial strains. Pseudomonas species, endowed with a versatile and rich metabolism, have been adopted for the conversion of glycerol into value-added products (ranging from simple molecules to structurally complex biopolymers, e.g. polyhydroxyalkanoates), and a number of metabolic engineering strategies have been deployed to increase the number of applications of glycerol as a cost-effective substrate. The unique genetic and metabolic features of glycerol-grown Pseudomonas are presented in this review, along with relevant examples of bioprocesses based on this substrate – and the synthetic biology and metabolic engineering strategies implemented in bacteria of this genus aimed at glycerol valorization.
AB - The use of renewable waste feedstocks is an environ-ment-friendly choice contributing to the reduction of waste treatment costs and increasing the economic value of industrial by-products. Glycerol (1,2,3-propa-netriol), a simple polyol compound widely distributed in biological systems, constitutes a prime example of a relatively cheap and readily available substrate to be used in bioprocesses. Extensively exploited as an ingredient in the food and pharmaceutical industries, glycerol is also the main by-product of biodiesel production, which has resulted in a progressive drop in substrate price over the years. Consequently, glycerol has become an attractive substrate in biotechnology, and several chemical commodities currently produced from petroleum have been shown to be obtained from this polyol using whole-cell biocata-lysts with both wild-type and engineered bacterial strains. Pseudomonas species, endowed with a versatile and rich metabolism, have been adopted for the conversion of glycerol into value-added products (ranging from simple molecules to structurally complex biopolymers, e.g. polyhydroxyalkanoates), and a number of metabolic engineering strategies have been deployed to increase the number of applications of glycerol as a cost-effective substrate. The unique genetic and metabolic features of glycerol-grown Pseudomonas are presented in this review, along with relevant examples of bioprocesses based on this substrate – and the synthetic biology and metabolic engineering strategies implemented in bacteria of this genus aimed at glycerol valorization.
UR - http://www.scopus.com/inward/record.url?scp=85064703747&partnerID=8YFLogxK
U2 - 10.1111/1751-7915.13400
DO - 10.1111/1751-7915.13400
M3 - Review article
C2 - 30883020
AN - SCOPUS:85064703747
SN - 1751-7907
VL - 13
SP - 32
EP - 53
JO - Microbial Biotechnology
JF - Microbial Biotechnology
IS - 1
ER -
