Binding of nuclear proteins to the promoter region of the laccase gene Cs-lcs1 from the basidiomycete Ceriporiopsis subvermispora

Rubén Polanco, Sergio Lobos, Rafael Vicuña

Resultado de la investigación: Article

12 Citas (Scopus)

Resumen

The white rot basidiomycete Ceriporiopsis subvermispora secretes the ligninolytic enzymes manganese-dependent peroxidase (MnP) and laccase to the extracellular medium. The promoter region of the laccase gene (Cs-lcs1) possesses several putative metal responsive elements (MRE), as well as a putative target site responding to copper termed ACE, similar to the one found in yeast. In this work, we show by electrophoretic mobility-shift assays that the migration of DNA probes containing either MRE sites or the ACE element are retarded in their mobility after incubation with nuclear extracts from C. subvermispora. Competition experiments suggested the presence of defined binding proteins recognizing these elements.

Idioma originalEnglish
Páginas (desde-hasta)525-528
Número de páginas4
PublicaciónEnzyme and Microbial Technology
Volumen30
N.º4
DOI
EstadoPublished - 16 abr 2002

Huella dactilar

Coriolaceae
Laccase
Basidiomycota
manganese peroxidase
Nuclear Proteins
Genetic Promoter Regions
Chemical elements
Carrier Proteins
Genes
Metals
Electrophoretic mobility
DNA Probes
Electrophoretic Mobility Shift Assay
Yeast
Copper
Assays
Yeasts
Manganese
DNA
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Applied Microbiology and Biotechnology

Citar esto

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Binding of nuclear proteins to the promoter region of the laccase gene Cs-lcs1 from the basidiomycete Ceriporiopsis subvermispora. / Polanco, Rubén; Lobos, Sergio; Vicuña, Rafael.

En: Enzyme and Microbial Technology, Vol. 30, N.º 4, 16.04.2002, p. 525-528.

Resultado de la investigación: Article

TY - JOUR

T1 - Binding of nuclear proteins to the promoter region of the laccase gene Cs-lcs1 from the basidiomycete Ceriporiopsis subvermispora

AU - Polanco, Rubén

AU - Lobos, Sergio

AU - Vicuña, Rafael

PY - 2002/4/16

Y1 - 2002/4/16

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AB - The white rot basidiomycete Ceriporiopsis subvermispora secretes the ligninolytic enzymes manganese-dependent peroxidase (MnP) and laccase to the extracellular medium. The promoter region of the laccase gene (Cs-lcs1) possesses several putative metal responsive elements (MRE), as well as a putative target site responding to copper termed ACE, similar to the one found in yeast. In this work, we show by electrophoretic mobility-shift assays that the migration of DNA probes containing either MRE sites or the ACE element are retarded in their mobility after incubation with nuclear extracts from C. subvermispora. Competition experiments suggested the presence of defined binding proteins recognizing these elements.

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DO - 10.1016/S0141-0229(01)00520-8

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JO - Enzyme and Microbial Technology

JF - Enzyme and Microbial Technology

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