Betaglycan expression is transcriptionally up-regulated during skeletal muscle differentiation: Cloning of murine betaglycan gene promoter and its modulation by MyoD, retinoic acid, and transforming growth factor-β

Fernando López-Casillas, Cecilia Riquelme, Yoshiaki Pérez-Kato, M. Veronica Ponce-Castaneda, Nelson Osses, Jose Esparza-Lopez, Gerardo Gonzalez-Nunez, Claudio Cabello-Verrugio, Valentín Mendoza, Victor Troncoso, Enrique Brandan

Resultado de la investigación: Article

37 Citas (Scopus)

Resumen

Betaglycan is a membrane-anchored proteoglycan coreceptor that binds transforming growth factor β (TGF-β) via its core protein and basic fibroblast growth factor through its glycosaminoglycan chains. In this study we evaluated the expression of betaglycan during the C2C12 skeletal muscle differentiation. Betaglycan expression, as determined by Northern and Western blot, was up-regulated during the conversion of myoblasts to myotubes. The mouse betaglycan gene promoter was cloned, and its sequence showed putative binding sites for SP1, Smad3, Smad4, muscle regulatory factor elements such as MyoD and MEF2, and retinoic acid receptor. Transcriptional activity of the mouse betaglycan promoter reporter was also up-regulated in differentiating C2C12 cells. We found that MyoD, but not myogenin, stimulated this transcriptional activity even in the presence of high serum. Betaglycan promoter activity was increased by RA and inhibited by the three isoforms of TGF-β. On the other hand, basic fibroblast growth factor, BMP-2, and hepatocyte growth factor/scatter factor, which are inhibitors of myogenesis, had little effect. In myotubes, up-regulated betaglycan was also detectable by TGF-β affinity labeling and immunofluorescence microscopy studies. The latter indicated that betaglycan was localized both on the cell surface and in the ECM. Forced expression of betaglycan in C2C12 myoblasts increases their responsiveness to TGF-β2, suggesting that it performs a TGF-β presentation function in this cell lineage. These results indicate that betaglycan expression is up-regulated during myogenesis and that MyoD and RA modulate its expression by a mechanism that is independent of myogenin.

Idioma originalEnglish
Páginas (desde-hasta)382-390
Número de páginas9
PublicaciónJournal of Biological Chemistry
Volumen278
N.º1
DOI
EstadoPublished - 3 ene 2003

Huella dactilar

Cloning
Transforming Growth Factors
Tretinoin
Muscle
Organism Cloning
Skeletal Muscle
Genes
Modulation
Fibroblast Growth Factor 2
Myogenin
Hepatocyte Growth Factor
Muscle Development
Myoblasts
Skeletal Muscle Fibers
betaglycan
Military electronic countermeasures
Retinoic Acid Receptors
Cell Lineage
Proteoglycans
Glycosaminoglycans

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Citar esto

López-Casillas, Fernando ; Riquelme, Cecilia ; Pérez-Kato, Yoshiaki ; Veronica Ponce-Castaneda, M. ; Osses, Nelson ; Esparza-Lopez, Jose ; Gonzalez-Nunez, Gerardo ; Cabello-Verrugio, Claudio ; Mendoza, Valentín ; Troncoso, Victor ; Brandan, Enrique. / Betaglycan expression is transcriptionally up-regulated during skeletal muscle differentiation : Cloning of murine betaglycan gene promoter and its modulation by MyoD, retinoic acid, and transforming growth factor-β. En: Journal of Biological Chemistry. 2003 ; Vol. 278, N.º 1. pp. 382-390.
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title = "Betaglycan expression is transcriptionally up-regulated during skeletal muscle differentiation: Cloning of murine betaglycan gene promoter and its modulation by MyoD, retinoic acid, and transforming growth factor-β",
abstract = "Betaglycan is a membrane-anchored proteoglycan coreceptor that binds transforming growth factor β (TGF-β) via its core protein and basic fibroblast growth factor through its glycosaminoglycan chains. In this study we evaluated the expression of betaglycan during the C2C12 skeletal muscle differentiation. Betaglycan expression, as determined by Northern and Western blot, was up-regulated during the conversion of myoblasts to myotubes. The mouse betaglycan gene promoter was cloned, and its sequence showed putative binding sites for SP1, Smad3, Smad4, muscle regulatory factor elements such as MyoD and MEF2, and retinoic acid receptor. Transcriptional activity of the mouse betaglycan promoter reporter was also up-regulated in differentiating C2C12 cells. We found that MyoD, but not myogenin, stimulated this transcriptional activity even in the presence of high serum. Betaglycan promoter activity was increased by RA and inhibited by the three isoforms of TGF-β. On the other hand, basic fibroblast growth factor, BMP-2, and hepatocyte growth factor/scatter factor, which are inhibitors of myogenesis, had little effect. In myotubes, up-regulated betaglycan was also detectable by TGF-β affinity labeling and immunofluorescence microscopy studies. The latter indicated that betaglycan was localized both on the cell surface and in the ECM. Forced expression of betaglycan in C2C12 myoblasts increases their responsiveness to TGF-β2, suggesting that it performs a TGF-β presentation function in this cell lineage. These results indicate that betaglycan expression is up-regulated during myogenesis and that MyoD and RA modulate its expression by a mechanism that is independent of myogenin.",
author = "Fernando L{\'o}pez-Casillas and Cecilia Riquelme and Yoshiaki P{\'e}rez-Kato and {Veronica Ponce-Castaneda}, M. and Nelson Osses and Jose Esparza-Lopez and Gerardo Gonzalez-Nunez and Claudio Cabello-Verrugio and Valent{\'i}n Mendoza and Victor Troncoso and Enrique Brandan",
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López-Casillas, F, Riquelme, C, Pérez-Kato, Y, Veronica Ponce-Castaneda, M, Osses, N, Esparza-Lopez, J, Gonzalez-Nunez, G, Cabello-Verrugio, C, Mendoza, V, Troncoso, V & Brandan, E 2003, 'Betaglycan expression is transcriptionally up-regulated during skeletal muscle differentiation: Cloning of murine betaglycan gene promoter and its modulation by MyoD, retinoic acid, and transforming growth factor-β', Journal of Biological Chemistry, vol. 278, n.º 1, pp. 382-390. https://doi.org/10.1074/jbc.M208520200

Betaglycan expression is transcriptionally up-regulated during skeletal muscle differentiation : Cloning of murine betaglycan gene promoter and its modulation by MyoD, retinoic acid, and transforming growth factor-β. / López-Casillas, Fernando; Riquelme, Cecilia; Pérez-Kato, Yoshiaki; Veronica Ponce-Castaneda, M.; Osses, Nelson; Esparza-Lopez, Jose; Gonzalez-Nunez, Gerardo; Cabello-Verrugio, Claudio; Mendoza, Valentín; Troncoso, Victor; Brandan, Enrique.

En: Journal of Biological Chemistry, Vol. 278, N.º 1, 03.01.2003, p. 382-390.

Resultado de la investigación: Article

TY - JOUR

T1 - Betaglycan expression is transcriptionally up-regulated during skeletal muscle differentiation

T2 - Cloning of murine betaglycan gene promoter and its modulation by MyoD, retinoic acid, and transforming growth factor-β

AU - López-Casillas, Fernando

AU - Riquelme, Cecilia

AU - Pérez-Kato, Yoshiaki

AU - Veronica Ponce-Castaneda, M.

AU - Osses, Nelson

AU - Esparza-Lopez, Jose

AU - Gonzalez-Nunez, Gerardo

AU - Cabello-Verrugio, Claudio

AU - Mendoza, Valentín

AU - Troncoso, Victor

AU - Brandan, Enrique

PY - 2003/1/3

Y1 - 2003/1/3

N2 - Betaglycan is a membrane-anchored proteoglycan coreceptor that binds transforming growth factor β (TGF-β) via its core protein and basic fibroblast growth factor through its glycosaminoglycan chains. In this study we evaluated the expression of betaglycan during the C2C12 skeletal muscle differentiation. Betaglycan expression, as determined by Northern and Western blot, was up-regulated during the conversion of myoblasts to myotubes. The mouse betaglycan gene promoter was cloned, and its sequence showed putative binding sites for SP1, Smad3, Smad4, muscle regulatory factor elements such as MyoD and MEF2, and retinoic acid receptor. Transcriptional activity of the mouse betaglycan promoter reporter was also up-regulated in differentiating C2C12 cells. We found that MyoD, but not myogenin, stimulated this transcriptional activity even in the presence of high serum. Betaglycan promoter activity was increased by RA and inhibited by the three isoforms of TGF-β. On the other hand, basic fibroblast growth factor, BMP-2, and hepatocyte growth factor/scatter factor, which are inhibitors of myogenesis, had little effect. In myotubes, up-regulated betaglycan was also detectable by TGF-β affinity labeling and immunofluorescence microscopy studies. The latter indicated that betaglycan was localized both on the cell surface and in the ECM. Forced expression of betaglycan in C2C12 myoblasts increases their responsiveness to TGF-β2, suggesting that it performs a TGF-β presentation function in this cell lineage. These results indicate that betaglycan expression is up-regulated during myogenesis and that MyoD and RA modulate its expression by a mechanism that is independent of myogenin.

AB - Betaglycan is a membrane-anchored proteoglycan coreceptor that binds transforming growth factor β (TGF-β) via its core protein and basic fibroblast growth factor through its glycosaminoglycan chains. In this study we evaluated the expression of betaglycan during the C2C12 skeletal muscle differentiation. Betaglycan expression, as determined by Northern and Western blot, was up-regulated during the conversion of myoblasts to myotubes. The mouse betaglycan gene promoter was cloned, and its sequence showed putative binding sites for SP1, Smad3, Smad4, muscle regulatory factor elements such as MyoD and MEF2, and retinoic acid receptor. Transcriptional activity of the mouse betaglycan promoter reporter was also up-regulated in differentiating C2C12 cells. We found that MyoD, but not myogenin, stimulated this transcriptional activity even in the presence of high serum. Betaglycan promoter activity was increased by RA and inhibited by the three isoforms of TGF-β. On the other hand, basic fibroblast growth factor, BMP-2, and hepatocyte growth factor/scatter factor, which are inhibitors of myogenesis, had little effect. In myotubes, up-regulated betaglycan was also detectable by TGF-β affinity labeling and immunofluorescence microscopy studies. The latter indicated that betaglycan was localized both on the cell surface and in the ECM. Forced expression of betaglycan in C2C12 myoblasts increases their responsiveness to TGF-β2, suggesting that it performs a TGF-β presentation function in this cell lineage. These results indicate that betaglycan expression is up-regulated during myogenesis and that MyoD and RA modulate its expression by a mechanism that is independent of myogenin.

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U2 - 10.1074/jbc.M208520200

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VL - 278

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EP - 390

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

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