Behaviour of RNA and protein synthesis during the acclimatization of the Carp. Studies with isolated hepatocytes

Lino Saez, Oscar Goicoechea, Rodolfo Amthauer, Manuel Krauskopf

Resultado de la investigación: Article

43 Citas (Scopus)

Resumen

1. 1. Carp hepatocytes were isolated by dissociating the liver tissue with collagenase. The procedure yields viable cells with highly preserved ultrastructural and metabolic features. The isolated cells were able to self-aggregate and form tissue. 2. 2. RNA and protein synthesis activity was significantly higher in the carp hepatocytes from summer acclimatized fish compared to the activity present in the cold adapted animal. 3. 3. RNA synthesis assayed in carp hepatocytes suspensions obtained from summer and winter acclimatized fish exhibited a behaviour consistent with an inverse compensation to the cold acclimatization state, being apparently repressed, whereas protein synthesis did not show a compensatory activity.

Idioma originalEnglish
Páginas (desde-hasta)31-38
Número de páginas8
PublicaciónComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volumen72
N.º1
DOI
EstadoPublished - 1982

Huella dactilar

Carps
Acclimatization
Hepatocytes
RNA
Fish
Fishes
Tissue
Proteins
Collagenases
Liver
Suspensions
Animals

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry
  • Molecular Biology
  • Physiology

Citar esto

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Behaviour of RNA and protein synthesis during the acclimatization of the Carp. Studies with isolated hepatocytes. / Saez, Lino; Goicoechea, Oscar; Amthauer, Rodolfo; Krauskopf, Manuel.

En: Comparative Biochemistry and Physiology -- Part B: Biochemistry and, Vol. 72, N.º 1, 1982, p. 31-38.

Resultado de la investigación: Article

TY - JOUR

T1 - Behaviour of RNA and protein synthesis during the acclimatization of the Carp. Studies with isolated hepatocytes

AU - Saez, Lino

AU - Goicoechea, Oscar

AU - Amthauer, Rodolfo

AU - Krauskopf, Manuel

PY - 1982

Y1 - 1982

N2 - 1. 1. Carp hepatocytes were isolated by dissociating the liver tissue with collagenase. The procedure yields viable cells with highly preserved ultrastructural and metabolic features. The isolated cells were able to self-aggregate and form tissue. 2. 2. RNA and protein synthesis activity was significantly higher in the carp hepatocytes from summer acclimatized fish compared to the activity present in the cold adapted animal. 3. 3. RNA synthesis assayed in carp hepatocytes suspensions obtained from summer and winter acclimatized fish exhibited a behaviour consistent with an inverse compensation to the cold acclimatization state, being apparently repressed, whereas protein synthesis did not show a compensatory activity.

AB - 1. 1. Carp hepatocytes were isolated by dissociating the liver tissue with collagenase. The procedure yields viable cells with highly preserved ultrastructural and metabolic features. The isolated cells were able to self-aggregate and form tissue. 2. 2. RNA and protein synthesis activity was significantly higher in the carp hepatocytes from summer acclimatized fish compared to the activity present in the cold adapted animal. 3. 3. RNA synthesis assayed in carp hepatocytes suspensions obtained from summer and winter acclimatized fish exhibited a behaviour consistent with an inverse compensation to the cold acclimatization state, being apparently repressed, whereas protein synthesis did not show a compensatory activity.

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U2 - 10.1016/0305-0491(82)90007-4

DO - 10.1016/0305-0491(82)90007-4

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VL - 72

SP - 31

EP - 38

JO - Comparative biochemistry and physiology. B, Comparative biochemistry

JF - Comparative biochemistry and physiology. B, Comparative biochemistry

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