Resumen
1. 1. Carp hepatocytes were isolated by dissociating the liver tissue with collagenase. The procedure yields viable cells with highly preserved ultrastructural and metabolic features. The isolated cells were able to self-aggregate and form tissue. 2. 2. RNA and protein synthesis activity was significantly higher in the carp hepatocytes from summer acclimatized fish compared to the activity present in the cold adapted animal. 3. 3. RNA synthesis assayed in carp hepatocytes suspensions obtained from summer and winter acclimatized fish exhibited a behaviour consistent with an inverse compensation to the cold acclimatization state, being apparently repressed, whereas protein synthesis did not show a compensatory activity.
| Idioma original | English |
|---|---|
| Páginas (desde-hasta) | 31-38 |
| Número de páginas | 8 |
| Publicación | Comparative Biochemistry and Physiology -- Part B: Biochemistry and |
| Volumen | 72 |
| N.º | 1 |
| DOI | |
| Estado | Published - 1982 |
Huella dactilar
ASJC Scopus subject areas
- Medicine(all)
- Biochemistry
- Molecular Biology
- Physiology
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Behaviour of RNA and protein synthesis during the acclimatization of the Carp. Studies with isolated hepatocytes. / Saez, Lino; Goicoechea, Oscar; Amthauer, Rodolfo; Krauskopf, Manuel.
En: Comparative Biochemistry and Physiology -- Part B: Biochemistry and, Vol. 72, N.º 1, 1982, p. 31-38.Resultado de la investigación: Article
TY - JOUR
T1 - Behaviour of RNA and protein synthesis during the acclimatization of the Carp. Studies with isolated hepatocytes
AU - Saez, Lino
AU - Goicoechea, Oscar
AU - Amthauer, Rodolfo
AU - Krauskopf, Manuel
PY - 1982
Y1 - 1982
N2 - 1. 1. Carp hepatocytes were isolated by dissociating the liver tissue with collagenase. The procedure yields viable cells with highly preserved ultrastructural and metabolic features. The isolated cells were able to self-aggregate and form tissue. 2. 2. RNA and protein synthesis activity was significantly higher in the carp hepatocytes from summer acclimatized fish compared to the activity present in the cold adapted animal. 3. 3. RNA synthesis assayed in carp hepatocytes suspensions obtained from summer and winter acclimatized fish exhibited a behaviour consistent with an inverse compensation to the cold acclimatization state, being apparently repressed, whereas protein synthesis did not show a compensatory activity.
AB - 1. 1. Carp hepatocytes were isolated by dissociating the liver tissue with collagenase. The procedure yields viable cells with highly preserved ultrastructural and metabolic features. The isolated cells were able to self-aggregate and form tissue. 2. 2. RNA and protein synthesis activity was significantly higher in the carp hepatocytes from summer acclimatized fish compared to the activity present in the cold adapted animal. 3. 3. RNA synthesis assayed in carp hepatocytes suspensions obtained from summer and winter acclimatized fish exhibited a behaviour consistent with an inverse compensation to the cold acclimatization state, being apparently repressed, whereas protein synthesis did not show a compensatory activity.
UR - http://www.scopus.com/inward/record.url?scp=0020023481&partnerID=8YFLogxK
U2 - 10.1016/0305-0491(82)90007-4
DO - 10.1016/0305-0491(82)90007-4
M3 - Article
C2 - 6179711
AN - SCOPUS:0020023481
VL - 72
SP - 31
EP - 38
JO - Comparative biochemistry and physiology. B, Comparative biochemistry
JF - Comparative biochemistry and physiology. B, Comparative biochemistry
SN - 1096-4959
IS - 1
ER -