TY - JOUR
T1 - AtUTr2 is an Arabidopsis thaliana nucleotide sugar transporter located in the Golgi apparatus capable of transporting UDP-galactose
AU - Norambuena, Lorena
AU - Nilo, Ricardo
AU - Handford, Michael
AU - Reyes, Francisca
AU - Marchant, Lorena
AU - Meisel, Lee
AU - Orellana, Ariel
N1 - Funding Information:
Acknowledgements We would like to thank Daniela Gómez for help in the experiments expressing AtUTr2 in tobacco and the people from the laboratory of Plant Molecular Genetics for helpful discussions. This work was supported by grants Fondecyt 2010066, 1000675, 1030551 and ICM P 02-009-F. Michael Handford was supported by The Leverhulme Trust. The GenBank accession number of the sequence reported in this paper is DQ015901
PY - 2005/10
Y1 - 2005/10
N2 - The synthesis of noncellulosic polysaccharides and glycoproteins in the plant cell Golgi apparatus requires UDP-galactose as a substrate. We have cloned and characterized a nucleotide sugar transporter from Arabidopsis thaliana (L.) Heynh. named AtUTr2. Expression in tobacco and Saccharomyces cerevisiae and subsequent biochemical characterization indicate that AtUTr2 transports UDP-galactose, but not UDP-glucose, UDP-N-acetyl glucosamine, UDP-xylose, UDP-glucuronic acid, GDP-fucose or GDP-mannose. Experiments expressing an AtUTr2-GFP fusion protein in onion epidermal cells suggest that AtUTr2 is located in the Golgi apparatus. Finally, northern analysis indicates that the AtUTr2 transcript was more abundant in roots and calli although it was also present in other Arabidopsis organs but at lower levels. Therefore, AtUTr2 is a nucleotide sugar transporter capable of transporting UDP-galactose that may play an important role in the synthesis of galactose-containing glycoconjugates in Arabidopsis.
AB - The synthesis of noncellulosic polysaccharides and glycoproteins in the plant cell Golgi apparatus requires UDP-galactose as a substrate. We have cloned and characterized a nucleotide sugar transporter from Arabidopsis thaliana (L.) Heynh. named AtUTr2. Expression in tobacco and Saccharomyces cerevisiae and subsequent biochemical characterization indicate that AtUTr2 transports UDP-galactose, but not UDP-glucose, UDP-N-acetyl glucosamine, UDP-xylose, UDP-glucuronic acid, GDP-fucose or GDP-mannose. Experiments expressing an AtUTr2-GFP fusion protein in onion epidermal cells suggest that AtUTr2 is located in the Golgi apparatus. Finally, northern analysis indicates that the AtUTr2 transcript was more abundant in roots and calli although it was also present in other Arabidopsis organs but at lower levels. Therefore, AtUTr2 is a nucleotide sugar transporter capable of transporting UDP-galactose that may play an important role in the synthesis of galactose-containing glycoconjugates in Arabidopsis.
KW - Arabidopsis thaliana
KW - Gene functional characterization
KW - Golgi apparatus
KW - Nucleotide sugar transporters
UR - http://www.scopus.com/inward/record.url?scp=26644433878&partnerID=8YFLogxK
U2 - 10.1007/s00425-005-1557-x
DO - 10.1007/s00425-005-1557-x
M3 - Article
C2 - 15891899
AN - SCOPUS:26644433878
SN - 0032-0935
VL - 222
SP - 521
EP - 529
JO - Planta
JF - Planta
IS - 3
ER -