ATP Induces IL-1 β Secretion in Neisseria gonorrhoeae-Infected Human Macrophages by a Mechanism Not Related to the NLRP3/ASC/Caspase-1 Axis

Killen Garci�, Gisselle Escobar, Pablo Mendoza, Caroll Beltran, Claudio Perez, Sergio Arancibia, Rolando Vernal, Paula I. Rodas, Claudio Acun�-Castillo, Alejandro Escobar

Resultado de la investigación: Article

1 Cita (Scopus)

Resumen

Neisseria gonorrhoeae (Ngo) has developed multiple immune evasion mechanisms involving the innate and adaptive immune responses. Recent findings have reported that Ngo reduces the IL-1β secretion of infected human monocyte-derived macrophages (MDM). Here, we investigate the role of adenosine triphosphate (ATP) in production and release of IL-1β in Ngo-infected MDM. We found that the exposure of Ngo-infected MDM to ATP increases IL-1β levels about ten times compared with unexposed Ngo-infected MDM (P<0.01). However, we did not observe any changes in inflammasome transcriptional activation of speck-like protein containing a caspase recruitment domain (CARD) (ASC, P>0.05) and caspase-1 (CASP1, P>0.05). In addition, ATP was not able to modify caspase-1 activity in Ngo-infected MDM but was able to increase pyroptosis (P>0.01). Notably ATP treatment defined an increase of positive staining for IL-1β with a distinctive intracellular pattern of distribution. Collectively, these data demonstrate that ATP induces IL-1β secretion by a mechanism not related to the NLRP3/ASC/caspase-1 axis and likely is acting at the level of vesicle trafficking or pore formation.

Idioma originalEnglish
Número de artículo1258504
PublicaciónMediators of Inflammation
Volumen2016
DOI
EstadoPublished - 1 ene 2016

Huella dactilar

Caspase 1
Neisseria gonorrhoeae
Interleukin-1
Adenosine Triphosphate
Macrophages
Immune Evasion
Adaptive Immunity
Innate Immunity
Staining and Labeling

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Citar esto

Garci�, Killen ; Escobar, Gisselle ; Mendoza, Pablo ; Beltran, Caroll ; Perez, Claudio ; Arancibia, Sergio ; Vernal, Rolando ; Rodas, Paula I. ; Acun�-Castillo, Claudio ; Escobar, Alejandro. / ATP Induces IL-1 β Secretion in Neisseria gonorrhoeae-Infected Human Macrophages by a Mechanism Not Related to the NLRP3/ASC/Caspase-1 Axis. En: Mediators of Inflammation. 2016 ; Vol. 2016.
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title = "ATP Induces IL-1 β Secretion in Neisseria gonorrhoeae-Infected Human Macrophages by a Mechanism Not Related to the NLRP3/ASC/Caspase-1 Axis",
abstract = "Neisseria gonorrhoeae (Ngo) has developed multiple immune evasion mechanisms involving the innate and adaptive immune responses. Recent findings have reported that Ngo reduces the IL-1β secretion of infected human monocyte-derived macrophages (MDM). Here, we investigate the role of adenosine triphosphate (ATP) in production and release of IL-1β in Ngo-infected MDM. We found that the exposure of Ngo-infected MDM to ATP increases IL-1β levels about ten times compared with unexposed Ngo-infected MDM (P<0.01). However, we did not observe any changes in inflammasome transcriptional activation of speck-like protein containing a caspase recruitment domain (CARD) (ASC, P>0.05) and caspase-1 (CASP1, P>0.05). In addition, ATP was not able to modify caspase-1 activity in Ngo-infected MDM but was able to increase pyroptosis (P>0.01). Notably ATP treatment defined an increase of positive staining for IL-1β with a distinctive intracellular pattern of distribution. Collectively, these data demonstrate that ATP induces IL-1β secretion by a mechanism not related to the NLRP3/ASC/caspase-1 axis and likely is acting at the level of vesicle trafficking or pore formation.",
author = "Killen Garci{\"i}¿½ and Gisselle Escobar and Pablo Mendoza and Caroll Beltran and Claudio Perez and Sergio Arancibia and Rolando Vernal and Rodas, {Paula I.} and Claudio Acun{\"i}¿½-Castillo and Alejandro Escobar",
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Garci�, K, Escobar, G, Mendoza, P, Beltran, C, Perez, C, Arancibia, S, Vernal, R, Rodas, PI, Acun�-Castillo, C & Escobar, A 2016, 'ATP Induces IL-1 β Secretion in Neisseria gonorrhoeae-Infected Human Macrophages by a Mechanism Not Related to the NLRP3/ASC/Caspase-1 Axis', Mediators of Inflammation, vol. 2016, 1258504. https://doi.org/10.1155/2016/1258504

ATP Induces IL-1 β Secretion in Neisseria gonorrhoeae-Infected Human Macrophages by a Mechanism Not Related to the NLRP3/ASC/Caspase-1 Axis. / Garci�, Killen; Escobar, Gisselle; Mendoza, Pablo; Beltran, Caroll; Perez, Claudio; Arancibia, Sergio; Vernal, Rolando; Rodas, Paula I.; Acun�-Castillo, Claudio; Escobar, Alejandro.

En: Mediators of Inflammation, Vol. 2016, 1258504, 01.01.2016.

Resultado de la investigación: Article

TY - JOUR

T1 - ATP Induces IL-1 β Secretion in Neisseria gonorrhoeae-Infected Human Macrophages by a Mechanism Not Related to the NLRP3/ASC/Caspase-1 Axis

AU - Garci�, Killen

AU - Escobar, Gisselle

AU - Mendoza, Pablo

AU - Beltran, Caroll

AU - Perez, Claudio

AU - Arancibia, Sergio

AU - Vernal, Rolando

AU - Rodas, Paula I.

AU - Acun�-Castillo, Claudio

AU - Escobar, Alejandro

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Neisseria gonorrhoeae (Ngo) has developed multiple immune evasion mechanisms involving the innate and adaptive immune responses. Recent findings have reported that Ngo reduces the IL-1β secretion of infected human monocyte-derived macrophages (MDM). Here, we investigate the role of adenosine triphosphate (ATP) in production and release of IL-1β in Ngo-infected MDM. We found that the exposure of Ngo-infected MDM to ATP increases IL-1β levels about ten times compared with unexposed Ngo-infected MDM (P<0.01). However, we did not observe any changes in inflammasome transcriptional activation of speck-like protein containing a caspase recruitment domain (CARD) (ASC, P>0.05) and caspase-1 (CASP1, P>0.05). In addition, ATP was not able to modify caspase-1 activity in Ngo-infected MDM but was able to increase pyroptosis (P>0.01). Notably ATP treatment defined an increase of positive staining for IL-1β with a distinctive intracellular pattern of distribution. Collectively, these data demonstrate that ATP induces IL-1β secretion by a mechanism not related to the NLRP3/ASC/caspase-1 axis and likely is acting at the level of vesicle trafficking or pore formation.

AB - Neisseria gonorrhoeae (Ngo) has developed multiple immune evasion mechanisms involving the innate and adaptive immune responses. Recent findings have reported that Ngo reduces the IL-1β secretion of infected human monocyte-derived macrophages (MDM). Here, we investigate the role of adenosine triphosphate (ATP) in production and release of IL-1β in Ngo-infected MDM. We found that the exposure of Ngo-infected MDM to ATP increases IL-1β levels about ten times compared with unexposed Ngo-infected MDM (P<0.01). However, we did not observe any changes in inflammasome transcriptional activation of speck-like protein containing a caspase recruitment domain (CARD) (ASC, P>0.05) and caspase-1 (CASP1, P>0.05). In addition, ATP was not able to modify caspase-1 activity in Ngo-infected MDM but was able to increase pyroptosis (P>0.01). Notably ATP treatment defined an increase of positive staining for IL-1β with a distinctive intracellular pattern of distribution. Collectively, these data demonstrate that ATP induces IL-1β secretion by a mechanism not related to the NLRP3/ASC/caspase-1 axis and likely is acting at the level of vesicle trafficking or pore formation.

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U2 - 10.1155/2016/1258504

DO - 10.1155/2016/1258504

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