Association of N-cadherin levels and downstream effectors of Rho GTPases with dendritic spine loss induced by chronic stress in rat hippocampal neurons

Patricia Castañeda, Mauricio Muñoz, Gonzalo García-Rojo, José L. Ulloa, Javier A. Bravo, Ruth Márquez, M. Alexandra García-Pérez, Damaris Arancibia, Karina Araneda, Paulina S. Rojas, David Mondaca-Ruff, Gabriela Díaz-Véliz, Sergio Mora, Esteban Aliaga, Jenny L. Fiedler

Resultado de la investigación: Contribución a una revistaArtículo

28 Citas (Scopus)

Resumen

Chronic stress promotes cognitive impairment and dendritic spine loss in hippocampal neurons. In this animal model of depression, spine loss probably involves a weakening of the interaction between pre- and postsynaptic cell adhesion molecules, such as N-cadherin, followed by disruption of the cytoskeleton. N-cadherin, in concert with catenin, stabilizes the cytoskeleton through Rho-family GTPases. Via their effector LIM kinase (LIMK), RhoA and ras-related C3 botulinum toxin substrate 1 (RAC) GTPases phosphorylate and inhibit cofilin, an actin-depolymerizing molecule, favoring spine growth. Additionally, RhoA, through Rho kinase (ROCK), inactivates myosin phosphatase through phosphorylation of the myosin-binding subunit (MYPT1), producing actomyosin contraction and probable spine loss. Some micro-RNAs negatively control the translation of specific mRNAs involved in Rho GTPase signaling. For example, miR-138 indirectly activates RhoA, and miR-134 reduces LIMK1 levels, resulting in spine shrinkage; in contrast, miR-132 activates RAC1, promoting spine formation. We evaluated whether N-cadherin/β-catenin and Rho signaling is sensitive to chronic restraint stress. Stressed rats exhibit anhedonia, impaired associative learning, and immobility in the forced swim test and reduction in N-cadherin levels but not β-catenin in the hippocampus. We observed a reduction in spine number in the apical dendrites of CA1 pyramidal neurons, with no effect on the levels of miR-132 or miR-134. Although the stress did not modify the RAC-LIMK-cofilin signaling pathway, we observed increased phospho-MYPT1 levels, probably mediated by RhoA-ROCK activation. Furthermore, chronic stress raises the levels of miR-138 in accordance with the observed activation of the RhoA-ROCK pathway. Our findings suggest that a dysregulation of RhoA-ROCK activity by chronic stress could potentially underlie spine loss in hippocampal neurons.

Idioma originalInglés
Páginas (desde-hasta)1476-1491
Número de páginas16
PublicaciónJournal of Neuroscience Research
Volumen93
N.º10
DOI
EstadoPublicada - 1 oct 2015

Áreas temáticas de ASJC Scopus

  • Neurociencia celular y molecular

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    Castañeda, P., Muñoz, M., García-Rojo, G., Ulloa, J. L., Bravo, J. A., Márquez, R., García-Pérez, M. A., Arancibia, D., Araneda, K., Rojas, P. S., Mondaca-Ruff, D., Díaz-Véliz, G., Mora, S., Aliaga, E., & Fiedler, J. L. (2015). Association of N-cadherin levels and downstream effectors of Rho GTPases with dendritic spine loss induced by chronic stress in rat hippocampal neurons. Journal of Neuroscience Research, 93(10), 1476-1491. https://doi.org/10.1002/jnr.23602