Assessing the effects of light on differentiation and virulence of the plant pathogen botrytis cinerea: Characterization of the white collar complex

Paulo Canessa, Julia Schumacher, Montserrat A. Hevia, Paul Tudzynski, Luis F. Larrondo

Resultado de la investigación: Article

66 Citas (Scopus)

Resumen

Organisms are exposed to a tough environment, where acute daily challenges, like light, can strongly affect several aspects of an individual's physiology, including pathogenesis. While several fungal models have been widely employed to understand the physiological and molecular events associated with light perception, various other agricultural-relevant fungi still remain, in terms of their responsiveness to light, in the dark. The fungus Botrytis cinerea is an aggressive pathogen able to cause disease on a wide range of plant species. Natural B. cinerea isolates exhibit a high degree of diversity in their predominant mode of reproduction. Thus, the majority of naturally occurring strains are known to reproduce asexually via conidia and sclerotia, and sexually via apothecia. Studies from the 1970's reported on specific developmental responses to treatments with near-UV, blue, red and far-red light. To unravel the signaling machinery triggering development - and possibly also connected with virulence - we initiated the functional characterization of the transcription factor/photoreceptor BcWCL1 and its partner BcWCL2, that form the White Collar Complex (WCC) in B. cinerea. Using mutants either abolished in or exhibiting enhanced WCC signaling (overexpression of both bcwcl1 and bcwcl2), we demonstrate that the WCC is an integral part of the mentioned machinery by mediating transcriptional responses to white light and the inhibition of conidiation in response to this stimulus. Furthermore, the WCC is required for coping with excessive light, oxidative stress and also to achieve full virulence. Although several transcriptional responses are abolished in the absence of bcwcl1, the expression of some genes is still light induced and a distinct conidiation pattern in response to daily light oscillations is enhanced, revealing a complex underlying photobiology. Though overlaps with well-studied fungal systems exist, the light-associated machinery of B. cinerea appears more complex than those of Neurospora crassa and Aspergillus nidulans.

Idioma originalEnglish
Número de artículoe84223
PublicaciónPLoS ONE
Volumen8
N.º12
DOI
EstadoPublished - 31 dic 2013

Huella dactilar

Botrytis
Pathogens
collars
Botrytis cinerea
plant pathogens
Virulence
virulence
Light
Machinery
Fungi
Aspergillus nidulans
Neurospora crassa
fungi
Photobiology
sclerotia
far-red light
white light
photoreceptors
Oxidative stress
Fungal Spores

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Citar esto

Canessa, Paulo ; Schumacher, Julia ; Hevia, Montserrat A. ; Tudzynski, Paul ; Larrondo, Luis F. / Assessing the effects of light on differentiation and virulence of the plant pathogen botrytis cinerea : Characterization of the white collar complex. En: PLoS ONE. 2013 ; Vol. 8, N.º 12.
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abstract = "Organisms are exposed to a tough environment, where acute daily challenges, like light, can strongly affect several aspects of an individual's physiology, including pathogenesis. While several fungal models have been widely employed to understand the physiological and molecular events associated with light perception, various other agricultural-relevant fungi still remain, in terms of their responsiveness to light, in the dark. The fungus Botrytis cinerea is an aggressive pathogen able to cause disease on a wide range of plant species. Natural B. cinerea isolates exhibit a high degree of diversity in their predominant mode of reproduction. Thus, the majority of naturally occurring strains are known to reproduce asexually via conidia and sclerotia, and sexually via apothecia. Studies from the 1970's reported on specific developmental responses to treatments with near-UV, blue, red and far-red light. To unravel the signaling machinery triggering development - and possibly also connected with virulence - we initiated the functional characterization of the transcription factor/photoreceptor BcWCL1 and its partner BcWCL2, that form the White Collar Complex (WCC) in B. cinerea. Using mutants either abolished in or exhibiting enhanced WCC signaling (overexpression of both bcwcl1 and bcwcl2), we demonstrate that the WCC is an integral part of the mentioned machinery by mediating transcriptional responses to white light and the inhibition of conidiation in response to this stimulus. Furthermore, the WCC is required for coping with excessive light, oxidative stress and also to achieve full virulence. Although several transcriptional responses are abolished in the absence of bcwcl1, the expression of some genes is still light induced and a distinct conidiation pattern in response to daily light oscillations is enhanced, revealing a complex underlying photobiology. Though overlaps with well-studied fungal systems exist, the light-associated machinery of B. cinerea appears more complex than those of Neurospora crassa and Aspergillus nidulans.",
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Assessing the effects of light on differentiation and virulence of the plant pathogen botrytis cinerea : Characterization of the white collar complex. / Canessa, Paulo; Schumacher, Julia; Hevia, Montserrat A.; Tudzynski, Paul; Larrondo, Luis F.

En: PLoS ONE, Vol. 8, N.º 12, e84223, 31.12.2013.

Resultado de la investigación: Article

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