Arsenic Response of Three Altiplanic Exiguobacterium Strains With Different Tolerance Levels Against the Metalloid Species: A Proteomics Study

Juan Castro-Severyn, Coral Pardo-Esté, Yoelvis Sulbaran, Carolina Cabezas, Valentina Gariazzo, Alan Briones, Naiyulin Morales, Martial Séveno, Mathilde Decourcelle, Nicolas Salvetat, Francisco Remonsellez, Eduardo Castro-Nallar, Franck Molina, Laurence Molina, Claudia P. Saavedra

Resultado de la investigación: Article

Resumen

Exiguobacterium is a polyextremophile bacterial genus with a physiology that allows it to develop in different adverse environments. The Salar de Huasco is one of these environments due to its altitude, atmospheric pressure, solar radiation, temperature variations, pH, salinity, and the presence of toxic compounds such as arsenic. However, the physiological and/or molecular mechanisms that enable them to prosper in these environments have not yet been described. Our research group has isolated several strains of Exiguobacterium genus from different sites of Salar de Huasco, which show different resistance levels to As(III) and As(V). In this work, we compare the protein expression patterns of the three strains in response to arsenic by a proteomic approach; strains were grown in absence of the metalloid and in presence of As(III) and As(V) sublethal concentrations and the protein separation was carried out in 2D electrophoresis gels (2D-GE). In total, 999 spots were detected, between 77 and 173 of which showed significant changes for As(III) among the three strains, and between 90 and 143 for As(V), respectively, compared to the corresponding control condition. Twenty-seven of those were identified by mass spectrometry (MS). Among these identified proteins, the ArsA [ATPase from the As(III) efflux pump] was found to be up-regulated in response to both arsenic conditions in the three strains, as well as the Co-enzyme A disulfide reductase (Cdr) in the two more resistant strains. Interestingly, in this genus the gene that codifies for Cdr is found within the genic context of the ars operon. We suggest that this protein could be restoring antioxidants molecules, necessary for the As(V) reduction. Additionally, among the proteins that change their expression against As, we found several with functions relevant to stress response, e.g., Hpf, LuxS, GLpX, GlnE, and Fur. This study allowed us to shed light into the physiology necessary for these bacteria to be able to tolerate the toxicity and stress generated by the presence of arsenic in their niche.

Idioma originalEnglish
Número de artículo2161
PublicaciónFrontiers in Microbiology
Volumen10
DOI
EstadoPublished - 26 sep 2019

Huella dactilar

Metalloids
Arsenic
Proteomics
Proteins
Disulfides
Oxidoreductases
Atmospheric Pressure
Poisons
Salinity
Electrophoresis, Gel, Two-Dimensional
Enzymes
Operon
Adenosine Triphosphatases
Mass Spectrometry
Antioxidants
Radiation
Bacteria
Temperature
Research
Genes

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Citar esto

Castro-Severyn, Juan ; Pardo-Esté, Coral ; Sulbaran, Yoelvis ; Cabezas, Carolina ; Gariazzo, Valentina ; Briones, Alan ; Morales, Naiyulin ; Séveno, Martial ; Decourcelle, Mathilde ; Salvetat, Nicolas ; Remonsellez, Francisco ; Castro-Nallar, Eduardo ; Molina, Franck ; Molina, Laurence ; Saavedra, Claudia P. / Arsenic Response of Three Altiplanic Exiguobacterium Strains With Different Tolerance Levels Against the Metalloid Species : A Proteomics Study. En: Frontiers in Microbiology. 2019 ; Vol. 10.
@article{3f935fa710c243298fc64caf4c17592a,
title = "Arsenic Response of Three Altiplanic Exiguobacterium Strains With Different Tolerance Levels Against the Metalloid Species: A Proteomics Study",
abstract = "Exiguobacterium is a polyextremophile bacterial genus with a physiology that allows it to develop in different adverse environments. The Salar de Huasco is one of these environments due to its altitude, atmospheric pressure, solar radiation, temperature variations, pH, salinity, and the presence of toxic compounds such as arsenic. However, the physiological and/or molecular mechanisms that enable them to prosper in these environments have not yet been described. Our research group has isolated several strains of Exiguobacterium genus from different sites of Salar de Huasco, which show different resistance levels to As(III) and As(V). In this work, we compare the protein expression patterns of the three strains in response to arsenic by a proteomic approach; strains were grown in absence of the metalloid and in presence of As(III) and As(V) sublethal concentrations and the protein separation was carried out in 2D electrophoresis gels (2D-GE). In total, 999 spots were detected, between 77 and 173 of which showed significant changes for As(III) among the three strains, and between 90 and 143 for As(V), respectively, compared to the corresponding control condition. Twenty-seven of those were identified by mass spectrometry (MS). Among these identified proteins, the ArsA [ATPase from the As(III) efflux pump] was found to be up-regulated in response to both arsenic conditions in the three strains, as well as the Co-enzyme A disulfide reductase (Cdr) in the two more resistant strains. Interestingly, in this genus the gene that codifies for Cdr is found within the genic context of the ars operon. We suggest that this protein could be restoring antioxidants molecules, necessary for the As(V) reduction. Additionally, among the proteins that change their expression against As, we found several with functions relevant to stress response, e.g., Hpf, LuxS, GLpX, GlnE, and Fur. This study allowed us to shed light into the physiology necessary for these bacteria to be able to tolerate the toxicity and stress generated by the presence of arsenic in their niche.",
keywords = "arsenic, Exiguobacterium, polyextremophile, proteomic, tolerance",
author = "Juan Castro-Severyn and Coral Pardo-Est{\'e} and Yoelvis Sulbaran and Carolina Cabezas and Valentina Gariazzo and Alan Briones and Naiyulin Morales and Martial S{\'e}veno and Mathilde Decourcelle and Nicolas Salvetat and Francisco Remonsellez and Eduardo Castro-Nallar and Franck Molina and Laurence Molina and Saavedra, {Claudia P.}",
year = "2019",
month = "9",
day = "26",
doi = "10.3389/fmicb.2019.02161",
language = "English",
volume = "10",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Media S.A.",

}

Castro-Severyn, J, Pardo-Esté, C, Sulbaran, Y, Cabezas, C, Gariazzo, V, Briones, A, Morales, N, Séveno, M, Decourcelle, M, Salvetat, N, Remonsellez, F, Castro-Nallar, E, Molina, F, Molina, L & Saavedra, CP 2019, 'Arsenic Response of Three Altiplanic Exiguobacterium Strains With Different Tolerance Levels Against the Metalloid Species: A Proteomics Study', Frontiers in Microbiology, vol. 10, 2161. https://doi.org/10.3389/fmicb.2019.02161

Arsenic Response of Three Altiplanic Exiguobacterium Strains With Different Tolerance Levels Against the Metalloid Species : A Proteomics Study. / Castro-Severyn, Juan; Pardo-Esté, Coral; Sulbaran, Yoelvis; Cabezas, Carolina; Gariazzo, Valentina; Briones, Alan; Morales, Naiyulin; Séveno, Martial; Decourcelle, Mathilde; Salvetat, Nicolas; Remonsellez, Francisco; Castro-Nallar, Eduardo; Molina, Franck; Molina, Laurence; Saavedra, Claudia P.

En: Frontiers in Microbiology, Vol. 10, 2161, 26.09.2019.

Resultado de la investigación: Article

TY - JOUR

T1 - Arsenic Response of Three Altiplanic Exiguobacterium Strains With Different Tolerance Levels Against the Metalloid Species

T2 - A Proteomics Study

AU - Castro-Severyn, Juan

AU - Pardo-Esté, Coral

AU - Sulbaran, Yoelvis

AU - Cabezas, Carolina

AU - Gariazzo, Valentina

AU - Briones, Alan

AU - Morales, Naiyulin

AU - Séveno, Martial

AU - Decourcelle, Mathilde

AU - Salvetat, Nicolas

AU - Remonsellez, Francisco

AU - Castro-Nallar, Eduardo

AU - Molina, Franck

AU - Molina, Laurence

AU - Saavedra, Claudia P.

PY - 2019/9/26

Y1 - 2019/9/26

N2 - Exiguobacterium is a polyextremophile bacterial genus with a physiology that allows it to develop in different adverse environments. The Salar de Huasco is one of these environments due to its altitude, atmospheric pressure, solar radiation, temperature variations, pH, salinity, and the presence of toxic compounds such as arsenic. However, the physiological and/or molecular mechanisms that enable them to prosper in these environments have not yet been described. Our research group has isolated several strains of Exiguobacterium genus from different sites of Salar de Huasco, which show different resistance levels to As(III) and As(V). In this work, we compare the protein expression patterns of the three strains in response to arsenic by a proteomic approach; strains were grown in absence of the metalloid and in presence of As(III) and As(V) sublethal concentrations and the protein separation was carried out in 2D electrophoresis gels (2D-GE). In total, 999 spots were detected, between 77 and 173 of which showed significant changes for As(III) among the three strains, and between 90 and 143 for As(V), respectively, compared to the corresponding control condition. Twenty-seven of those were identified by mass spectrometry (MS). Among these identified proteins, the ArsA [ATPase from the As(III) efflux pump] was found to be up-regulated in response to both arsenic conditions in the three strains, as well as the Co-enzyme A disulfide reductase (Cdr) in the two more resistant strains. Interestingly, in this genus the gene that codifies for Cdr is found within the genic context of the ars operon. We suggest that this protein could be restoring antioxidants molecules, necessary for the As(V) reduction. Additionally, among the proteins that change their expression against As, we found several with functions relevant to stress response, e.g., Hpf, LuxS, GLpX, GlnE, and Fur. This study allowed us to shed light into the physiology necessary for these bacteria to be able to tolerate the toxicity and stress generated by the presence of arsenic in their niche.

AB - Exiguobacterium is a polyextremophile bacterial genus with a physiology that allows it to develop in different adverse environments. The Salar de Huasco is one of these environments due to its altitude, atmospheric pressure, solar radiation, temperature variations, pH, salinity, and the presence of toxic compounds such as arsenic. However, the physiological and/or molecular mechanisms that enable them to prosper in these environments have not yet been described. Our research group has isolated several strains of Exiguobacterium genus from different sites of Salar de Huasco, which show different resistance levels to As(III) and As(V). In this work, we compare the protein expression patterns of the three strains in response to arsenic by a proteomic approach; strains were grown in absence of the metalloid and in presence of As(III) and As(V) sublethal concentrations and the protein separation was carried out in 2D electrophoresis gels (2D-GE). In total, 999 spots were detected, between 77 and 173 of which showed significant changes for As(III) among the three strains, and between 90 and 143 for As(V), respectively, compared to the corresponding control condition. Twenty-seven of those were identified by mass spectrometry (MS). Among these identified proteins, the ArsA [ATPase from the As(III) efflux pump] was found to be up-regulated in response to both arsenic conditions in the three strains, as well as the Co-enzyme A disulfide reductase (Cdr) in the two more resistant strains. Interestingly, in this genus the gene that codifies for Cdr is found within the genic context of the ars operon. We suggest that this protein could be restoring antioxidants molecules, necessary for the As(V) reduction. Additionally, among the proteins that change their expression against As, we found several with functions relevant to stress response, e.g., Hpf, LuxS, GLpX, GlnE, and Fur. This study allowed us to shed light into the physiology necessary for these bacteria to be able to tolerate the toxicity and stress generated by the presence of arsenic in their niche.

KW - arsenic

KW - Exiguobacterium

KW - polyextremophile

KW - proteomic

KW - tolerance

UR - http://www.scopus.com/inward/record.url?scp=85073121457&partnerID=8YFLogxK

U2 - 10.3389/fmicb.2019.02161

DO - 10.3389/fmicb.2019.02161

M3 - Article

AN - SCOPUS:85073121457

VL - 10

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

M1 - 2161

ER -