Arcobacter ebronensis sp. nov. and Arcobacter aquimarinus sp. nov., two new species isolated from marine environment

Arturo Levican, Sara Rubio-Arcos, Antonio Martinez-Murcia, Luis Collado, María José Figueras

Resultado de la investigación: Article

34 Citas (Scopus)

Resumen

Two strains recovered from mussels (F128-2T) and sea water (W63T) were characterized as Arcobacter sp., but they could not be assigned to any known species using the molecular identification methods specific for this genus (16S rDNA-RFLP and m-PCR) and rpoB gene analysis. The 16S rRNA gene sequence similarity to the type strains of all Arcobacter species ranged from 92.2% to 96.7% with strain F128-2T, and from 94.1% to 99.4% with strain W63T, the most similar being A. bivalviorum (CECT 7835T) and A. defluvii (CECT 7697T), respectively. The phylogenetic analyses of 16S rRNA, and the concatenated sequences of gyrB, gyrA, rpoB, atpA and hsp60 genes confirmed that strains F128-2T and W63T belonged to two new lineages within the genus Arcobacter. Moreover, both strains showed differential phenotypic characteristics and MALDI-TOF mass spectra from all other Arcobacter species. Therefore, it has been demonstrated the existence of two new Arcobacter species and the proposed names are Arcobacter ebronensis (type strain F128-2T=CECT 8441T=LMG 27922T), and Arcobacter aquimarinus (type strain W63T=CECT 8442T=LMG 27923T).

Idioma originalEnglish
Páginas (desde-hasta)30-35
Número de páginas6
PublicaciónSystematic and Applied Microbiology
Volumen38
N.º1
DOI
EstadoPublished - 1 feb 2015
Publicado de forma externa

Huella dactilar

Arcobacter
marine environment
new species
gene
identification method
seawater
phylogenetics
ribosomal RNA
Bivalvia
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Seawater
Ribosomal DNA
rRNA Genes
Restriction Fragment Length Polymorphisms
Genes
Names
mussels
restriction fragment length polymorphism
genes
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Applied Microbiology and Biotechnology

Citar esto

Levican, Arturo ; Rubio-Arcos, Sara ; Martinez-Murcia, Antonio ; Collado, Luis ; Figueras, María José. / Arcobacter ebronensis sp. nov. and Arcobacter aquimarinus sp. nov., two new species isolated from marine environment. En: Systematic and Applied Microbiology. 2015 ; Vol. 38, N.º 1. pp. 30-35.
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abstract = "Two strains recovered from mussels (F128-2T) and sea water (W63T) were characterized as Arcobacter sp., but they could not be assigned to any known species using the molecular identification methods specific for this genus (16S rDNA-RFLP and m-PCR) and rpoB gene analysis. The 16S rRNA gene sequence similarity to the type strains of all Arcobacter species ranged from 92.2{\%} to 96.7{\%} with strain F128-2T, and from 94.1{\%} to 99.4{\%} with strain W63T, the most similar being A. bivalviorum (CECT 7835T) and A. defluvii (CECT 7697T), respectively. The phylogenetic analyses of 16S rRNA, and the concatenated sequences of gyrB, gyrA, rpoB, atpA and hsp60 genes confirmed that strains F128-2T and W63T belonged to two new lineages within the genus Arcobacter. Moreover, both strains showed differential phenotypic characteristics and MALDI-TOF mass spectra from all other Arcobacter species. Therefore, it has been demonstrated the existence of two new Arcobacter species and the proposed names are Arcobacter ebronensis (type strain F128-2T=CECT 8441T=LMG 27922T), and Arcobacter aquimarinus (type strain W63T=CECT 8442T=LMG 27923T).",
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Arcobacter ebronensis sp. nov. and Arcobacter aquimarinus sp. nov., two new species isolated from marine environment. / Levican, Arturo; Rubio-Arcos, Sara; Martinez-Murcia, Antonio; Collado, Luis; Figueras, María José.

En: Systematic and Applied Microbiology, Vol. 38, N.º 1, 01.02.2015, p. 30-35.

Resultado de la investigación: Article

TY - JOUR

T1 - Arcobacter ebronensis sp. nov. and Arcobacter aquimarinus sp. nov., two new species isolated from marine environment

AU - Levican, Arturo

AU - Rubio-Arcos, Sara

AU - Martinez-Murcia, Antonio

AU - Collado, Luis

AU - Figueras, María José

PY - 2015/2/1

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N2 - Two strains recovered from mussels (F128-2T) and sea water (W63T) were characterized as Arcobacter sp., but they could not be assigned to any known species using the molecular identification methods specific for this genus (16S rDNA-RFLP and m-PCR) and rpoB gene analysis. The 16S rRNA gene sequence similarity to the type strains of all Arcobacter species ranged from 92.2% to 96.7% with strain F128-2T, and from 94.1% to 99.4% with strain W63T, the most similar being A. bivalviorum (CECT 7835T) and A. defluvii (CECT 7697T), respectively. The phylogenetic analyses of 16S rRNA, and the concatenated sequences of gyrB, gyrA, rpoB, atpA and hsp60 genes confirmed that strains F128-2T and W63T belonged to two new lineages within the genus Arcobacter. Moreover, both strains showed differential phenotypic characteristics and MALDI-TOF mass spectra from all other Arcobacter species. Therefore, it has been demonstrated the existence of two new Arcobacter species and the proposed names are Arcobacter ebronensis (type strain F128-2T=CECT 8441T=LMG 27922T), and Arcobacter aquimarinus (type strain W63T=CECT 8442T=LMG 27923T).

AB - Two strains recovered from mussels (F128-2T) and sea water (W63T) were characterized as Arcobacter sp., but they could not be assigned to any known species using the molecular identification methods specific for this genus (16S rDNA-RFLP and m-PCR) and rpoB gene analysis. The 16S rRNA gene sequence similarity to the type strains of all Arcobacter species ranged from 92.2% to 96.7% with strain F128-2T, and from 94.1% to 99.4% with strain W63T, the most similar being A. bivalviorum (CECT 7835T) and A. defluvii (CECT 7697T), respectively. The phylogenetic analyses of 16S rRNA, and the concatenated sequences of gyrB, gyrA, rpoB, atpA and hsp60 genes confirmed that strains F128-2T and W63T belonged to two new lineages within the genus Arcobacter. Moreover, both strains showed differential phenotypic characteristics and MALDI-TOF mass spectra from all other Arcobacter species. Therefore, it has been demonstrated the existence of two new Arcobacter species and the proposed names are Arcobacter ebronensis (type strain F128-2T=CECT 8441T=LMG 27922T), and Arcobacter aquimarinus (type strain W63T=CECT 8442T=LMG 27923T).

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