Resumen
Lipid phosphate phosphatases (LPPs, E.C. 3.1.3.4) catalyse the dephosphorylation of diacylglycerol pyrophosphate (DGPP) and phosphatidic acid (PA), which are secondary messengers in abscisic acid (ABA) signalling. In this study, we investigated the effect of ABA on the expression of AtLPP genes as they encode putative ABA-signalling partners. We observed that AtLPP2 expression was down-regulated by ABA and we performed experiments on Atlpp2-2, an AtLPP2 knockout mutant, to determine whether AtLPP2 was involved in ABA signalling. We observed that Atlpp2-2 plantlets contained about twice as much PA as the wild-type Col-0 and exhibited higher PA kinase (PAK) activity than Col-0 plants. In addition, we showed that ABA stimulated diacylglycerol kinase (DGK) activity independently of AtLPP2 activity but that the ABA-stimulation of PAK activity recorded in Col-0 was dependent on AtLPP2. In order to evaluate the involvement of AtLPP2 activity in guard cell function, we measured the ABA sensitivity of Atlpp2-2 stomata. The inhibition of stomatal opening was less sensitive to ABA in Atlpp2-2 than in Col-0. Watered and water-stressed plants of the two genotypes accumulated ABA to the same extent, thus leading us to consider Atlpp2-2 an ABA-signalling mutant. Taken together our observations show that AtLPP2 is a part of ABA signalling and participate to the regulation of stomatal movements.
| Idioma original | English |
|---|---|
| Páginas (desde-hasta) | 357-362 |
| Número de páginas | 6 |
| Publicación | Plant Physiology and Biochemistry |
| Volumen | 49 |
| N.º | 3 |
| DOI | |
| Estado | Published - 1 mar 2011 |
Huella dactilar
ASJC Scopus subject areas
- Physiology
- Genetics
- Plant Science
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Arabidopsis thaliana lipid phosphate phosphatase 2 is involved in abscisic acid signalling in leaves. / Paradis, Sophie; Villasuso, Ana Laura; Aguayo, Susana Saez; Maldiney, Régis; Habricot, Yvette; Zalejski, Christine; Machado, Estela; Sotta, Bruno; Miginiac, Emile; Jeannette, Emmanuelle.
En: Plant Physiology and Biochemistry, Vol. 49, N.º 3, 01.03.2011, p. 357-362.Resultado de la investigación: Article
TY - JOUR
T1 - Arabidopsis thaliana lipid phosphate phosphatase 2 is involved in abscisic acid signalling in leaves
AU - Paradis, Sophie
AU - Villasuso, Ana Laura
AU - Aguayo, Susana Saez
AU - Maldiney, Régis
AU - Habricot, Yvette
AU - Zalejski, Christine
AU - Machado, Estela
AU - Sotta, Bruno
AU - Miginiac, Emile
AU - Jeannette, Emmanuelle
PY - 2011/3/1
Y1 - 2011/3/1
N2 - Lipid phosphate phosphatases (LPPs, E.C. 3.1.3.4) catalyse the dephosphorylation of diacylglycerol pyrophosphate (DGPP) and phosphatidic acid (PA), which are secondary messengers in abscisic acid (ABA) signalling. In this study, we investigated the effect of ABA on the expression of AtLPP genes as they encode putative ABA-signalling partners. We observed that AtLPP2 expression was down-regulated by ABA and we performed experiments on Atlpp2-2, an AtLPP2 knockout mutant, to determine whether AtLPP2 was involved in ABA signalling. We observed that Atlpp2-2 plantlets contained about twice as much PA as the wild-type Col-0 and exhibited higher PA kinase (PAK) activity than Col-0 plants. In addition, we showed that ABA stimulated diacylglycerol kinase (DGK) activity independently of AtLPP2 activity but that the ABA-stimulation of PAK activity recorded in Col-0 was dependent on AtLPP2. In order to evaluate the involvement of AtLPP2 activity in guard cell function, we measured the ABA sensitivity of Atlpp2-2 stomata. The inhibition of stomatal opening was less sensitive to ABA in Atlpp2-2 than in Col-0. Watered and water-stressed plants of the two genotypes accumulated ABA to the same extent, thus leading us to consider Atlpp2-2 an ABA-signalling mutant. Taken together our observations show that AtLPP2 is a part of ABA signalling and participate to the regulation of stomatal movements.
AB - Lipid phosphate phosphatases (LPPs, E.C. 3.1.3.4) catalyse the dephosphorylation of diacylglycerol pyrophosphate (DGPP) and phosphatidic acid (PA), which are secondary messengers in abscisic acid (ABA) signalling. In this study, we investigated the effect of ABA on the expression of AtLPP genes as they encode putative ABA-signalling partners. We observed that AtLPP2 expression was down-regulated by ABA and we performed experiments on Atlpp2-2, an AtLPP2 knockout mutant, to determine whether AtLPP2 was involved in ABA signalling. We observed that Atlpp2-2 plantlets contained about twice as much PA as the wild-type Col-0 and exhibited higher PA kinase (PAK) activity than Col-0 plants. In addition, we showed that ABA stimulated diacylglycerol kinase (DGK) activity independently of AtLPP2 activity but that the ABA-stimulation of PAK activity recorded in Col-0 was dependent on AtLPP2. In order to evaluate the involvement of AtLPP2 activity in guard cell function, we measured the ABA sensitivity of Atlpp2-2 stomata. The inhibition of stomatal opening was less sensitive to ABA in Atlpp2-2 than in Col-0. Watered and water-stressed plants of the two genotypes accumulated ABA to the same extent, thus leading us to consider Atlpp2-2 an ABA-signalling mutant. Taken together our observations show that AtLPP2 is a part of ABA signalling and participate to the regulation of stomatal movements.
KW - Abscisic acid
KW - Arabidopsis
KW - Diacylglycerol kinase
KW - Lipid phosphate phosphatase
KW - Phosphatidic acid
KW - Phosphatidic acid kinase
KW - Stomatal movements
UR - http://www.scopus.com/inward/record.url?scp=79951678079&partnerID=8YFLogxK
U2 - 10.1016/j.plaphy.2011.01.010
DO - 10.1016/j.plaphy.2011.01.010
M3 - Article
C2 - 21277215
AN - SCOPUS:79951678079
VL - 49
SP - 357
EP - 362
JO - Plant Physiology and Biochemistry
JF - Plant Physiology and Biochemistry
SN - 0981-9428
IS - 3
ER -