Arabidopsis thaliana lipid phosphate phosphatase 2 is involved in abscisic acid signalling in leaves

Sophie Paradis, Ana Laura Villasuso, Susana Saez Aguayo, Régis Maldiney, Yvette Habricot, Christine Zalejski, Estela Machado, Bruno Sotta, Emile Miginiac, Emmanuelle Jeannette

Resultado de la investigación: Article

17 Citas (Scopus)

Resumen

Lipid phosphate phosphatases (LPPs, E.C. 3.1.3.4) catalyse the dephosphorylation of diacylglycerol pyrophosphate (DGPP) and phosphatidic acid (PA), which are secondary messengers in abscisic acid (ABA) signalling. In this study, we investigated the effect of ABA on the expression of AtLPP genes as they encode putative ABA-signalling partners. We observed that AtLPP2 expression was down-regulated by ABA and we performed experiments on Atlpp2-2, an AtLPP2 knockout mutant, to determine whether AtLPP2 was involved in ABA signalling. We observed that Atlpp2-2 plantlets contained about twice as much PA as the wild-type Col-0 and exhibited higher PA kinase (PAK) activity than Col-0 plants. In addition, we showed that ABA stimulated diacylglycerol kinase (DGK) activity independently of AtLPP2 activity but that the ABA-stimulation of PAK activity recorded in Col-0 was dependent on AtLPP2. In order to evaluate the involvement of AtLPP2 activity in guard cell function, we measured the ABA sensitivity of Atlpp2-2 stomata. The inhibition of stomatal opening was less sensitive to ABA in Atlpp2-2 than in Col-0. Watered and water-stressed plants of the two genotypes accumulated ABA to the same extent, thus leading us to consider Atlpp2-2 an ABA-signalling mutant. Taken together our observations show that AtLPP2 is a part of ABA signalling and participate to the regulation of stomatal movements.

Idioma originalEnglish
Páginas (desde-hasta)357-362
Número de páginas6
PublicaciónPlant Physiology and Biochemistry
Volumen49
N.º3
DOI
EstadoPublished - 1 mar 2011

Huella dactilar

Abscisic Acid
Arabidopsis
abscisic acid
Arabidopsis thaliana
phosphates
lipids
leaves
Phosphatidic Acids
lipid phosphate phosphatase
phosphatidate phosphatase
acids
phosphotransferases (kinases)
diacylglycerol kinase
Diacylglycerol Kinase
stomatal movement
knockout mutants
dephosphorylation
pyrophosphates
guard cells
diacylglycerols

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Citar esto

Paradis, Sophie ; Villasuso, Ana Laura ; Aguayo, Susana Saez ; Maldiney, Régis ; Habricot, Yvette ; Zalejski, Christine ; Machado, Estela ; Sotta, Bruno ; Miginiac, Emile ; Jeannette, Emmanuelle. / Arabidopsis thaliana lipid phosphate phosphatase 2 is involved in abscisic acid signalling in leaves. En: Plant Physiology and Biochemistry. 2011 ; Vol. 49, N.º 3. pp. 357-362.
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title = "Arabidopsis thaliana lipid phosphate phosphatase 2 is involved in abscisic acid signalling in leaves",
abstract = "Lipid phosphate phosphatases (LPPs, E.C. 3.1.3.4) catalyse the dephosphorylation of diacylglycerol pyrophosphate (DGPP) and phosphatidic acid (PA), which are secondary messengers in abscisic acid (ABA) signalling. In this study, we investigated the effect of ABA on the expression of AtLPP genes as they encode putative ABA-signalling partners. We observed that AtLPP2 expression was down-regulated by ABA and we performed experiments on Atlpp2-2, an AtLPP2 knockout mutant, to determine whether AtLPP2 was involved in ABA signalling. We observed that Atlpp2-2 plantlets contained about twice as much PA as the wild-type Col-0 and exhibited higher PA kinase (PAK) activity than Col-0 plants. In addition, we showed that ABA stimulated diacylglycerol kinase (DGK) activity independently of AtLPP2 activity but that the ABA-stimulation of PAK activity recorded in Col-0 was dependent on AtLPP2. In order to evaluate the involvement of AtLPP2 activity in guard cell function, we measured the ABA sensitivity of Atlpp2-2 stomata. The inhibition of stomatal opening was less sensitive to ABA in Atlpp2-2 than in Col-0. Watered and water-stressed plants of the two genotypes accumulated ABA to the same extent, thus leading us to consider Atlpp2-2 an ABA-signalling mutant. Taken together our observations show that AtLPP2 is a part of ABA signalling and participate to the regulation of stomatal movements.",
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Paradis, S, Villasuso, AL, Aguayo, SS, Maldiney, R, Habricot, Y, Zalejski, C, Machado, E, Sotta, B, Miginiac, E & Jeannette, E 2011, 'Arabidopsis thaliana lipid phosphate phosphatase 2 is involved in abscisic acid signalling in leaves', Plant Physiology and Biochemistry, vol. 49, n.º 3, pp. 357-362. https://doi.org/10.1016/j.plaphy.2011.01.010

Arabidopsis thaliana lipid phosphate phosphatase 2 is involved in abscisic acid signalling in leaves. / Paradis, Sophie; Villasuso, Ana Laura; Aguayo, Susana Saez; Maldiney, Régis; Habricot, Yvette; Zalejski, Christine; Machado, Estela; Sotta, Bruno; Miginiac, Emile; Jeannette, Emmanuelle.

En: Plant Physiology and Biochemistry, Vol. 49, N.º 3, 01.03.2011, p. 357-362.

Resultado de la investigación: Article

TY - JOUR

T1 - Arabidopsis thaliana lipid phosphate phosphatase 2 is involved in abscisic acid signalling in leaves

AU - Paradis, Sophie

AU - Villasuso, Ana Laura

AU - Aguayo, Susana Saez

AU - Maldiney, Régis

AU - Habricot, Yvette

AU - Zalejski, Christine

AU - Machado, Estela

AU - Sotta, Bruno

AU - Miginiac, Emile

AU - Jeannette, Emmanuelle

PY - 2011/3/1

Y1 - 2011/3/1

N2 - Lipid phosphate phosphatases (LPPs, E.C. 3.1.3.4) catalyse the dephosphorylation of diacylglycerol pyrophosphate (DGPP) and phosphatidic acid (PA), which are secondary messengers in abscisic acid (ABA) signalling. In this study, we investigated the effect of ABA on the expression of AtLPP genes as they encode putative ABA-signalling partners. We observed that AtLPP2 expression was down-regulated by ABA and we performed experiments on Atlpp2-2, an AtLPP2 knockout mutant, to determine whether AtLPP2 was involved in ABA signalling. We observed that Atlpp2-2 plantlets contained about twice as much PA as the wild-type Col-0 and exhibited higher PA kinase (PAK) activity than Col-0 plants. In addition, we showed that ABA stimulated diacylglycerol kinase (DGK) activity independently of AtLPP2 activity but that the ABA-stimulation of PAK activity recorded in Col-0 was dependent on AtLPP2. In order to evaluate the involvement of AtLPP2 activity in guard cell function, we measured the ABA sensitivity of Atlpp2-2 stomata. The inhibition of stomatal opening was less sensitive to ABA in Atlpp2-2 than in Col-0. Watered and water-stressed plants of the two genotypes accumulated ABA to the same extent, thus leading us to consider Atlpp2-2 an ABA-signalling mutant. Taken together our observations show that AtLPP2 is a part of ABA signalling and participate to the regulation of stomatal movements.

AB - Lipid phosphate phosphatases (LPPs, E.C. 3.1.3.4) catalyse the dephosphorylation of diacylglycerol pyrophosphate (DGPP) and phosphatidic acid (PA), which are secondary messengers in abscisic acid (ABA) signalling. In this study, we investigated the effect of ABA on the expression of AtLPP genes as they encode putative ABA-signalling partners. We observed that AtLPP2 expression was down-regulated by ABA and we performed experiments on Atlpp2-2, an AtLPP2 knockout mutant, to determine whether AtLPP2 was involved in ABA signalling. We observed that Atlpp2-2 plantlets contained about twice as much PA as the wild-type Col-0 and exhibited higher PA kinase (PAK) activity than Col-0 plants. In addition, we showed that ABA stimulated diacylglycerol kinase (DGK) activity independently of AtLPP2 activity but that the ABA-stimulation of PAK activity recorded in Col-0 was dependent on AtLPP2. In order to evaluate the involvement of AtLPP2 activity in guard cell function, we measured the ABA sensitivity of Atlpp2-2 stomata. The inhibition of stomatal opening was less sensitive to ABA in Atlpp2-2 than in Col-0. Watered and water-stressed plants of the two genotypes accumulated ABA to the same extent, thus leading us to consider Atlpp2-2 an ABA-signalling mutant. Taken together our observations show that AtLPP2 is a part of ABA signalling and participate to the regulation of stomatal movements.

KW - Abscisic acid

KW - Arabidopsis

KW - Diacylglycerol kinase

KW - Lipid phosphate phosphatase

KW - Phosphatidic acid

KW - Phosphatidic acid kinase

KW - Stomatal movements

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U2 - 10.1016/j.plaphy.2011.01.010

DO - 10.1016/j.plaphy.2011.01.010

M3 - Article

C2 - 21277215

AN - SCOPUS:79951678079

VL - 49

SP - 357

EP - 362

JO - Plant Physiology and Biochemistry

JF - Plant Physiology and Biochemistry

SN - 0981-9428

IS - 3

ER -