Arabidopsis thaliana AtUTr7 encodes a golgi-localized UDP-glucose/UDP- galactose transporter that affects lateral root emergence

Michael Handford, Cecilia Rodríguez-Furlán, Lorena Marchant, Marcelo Segura, Daniela Gómez, Elena Alvarez-Buylla, Guang Yan Xiong, Markus Pauly, Ariel Orellana

Resultado de la investigación: Article

18 Citas (Scopus)

Resumen

Nucleotide sugar transporters (NSTs) are antiporters comprising a gene family that plays a fundamental role in the biosynthesis of complex cell wall polysaccharides and glycoproteins in plants. However, due to the limited number of related mutants that have observable phenotypes, the biological function(s) of most NSTs in cell wall biosynthesis and assembly have remained elusive. Here, we report the characterization of AtUTr7 from Arabidopsis (Arabidopsis thaliana (L.) Heynh.), which is homologous to multi-specific UDP-sugar transporters from Drosophila melanogaster, humans, and Caenorhabditis elegans. We show that AtUTr7 possesses the common structural characteristics conserved among NSTs. Using a green fluorescent protein (GFP) tagged version, we demonstrate that AtUTr7 is localized in the Golgi apparatus. We also show that AtUTr7 is widely expressed, especially in the roots and in specific floral organs. Additionally, the results of an in vitro nucleotide sugar transport assay carried out with a tobacco and a yeast expression system suggest that AtUTr7 is capable of transferring UDP-Gal and UDP-Glc, but not a range of other UDP- and GDP-sugars, into the Golgi lumen. Mutants lacking expression of AtUTr7 exhibited an early proliferation of lateral roots as well as distorted root hairs when cultivated at high sucrose concentrations. Furthermore, the distribution of homogalacturonan with a low degree of methyl esterification differed in lateral root tips of the mutant compared to wild-type plants, although additional analytical procedures revealed no further differences in the composition of the root cell walls. This evidence suggests that the transport of UDP-Gal and UDP-Glc into the Golgi under conditions of high root biomass production plays a role in lateral root and root hair development.

Idioma originalEnglish
Páginas (desde-hasta)1263-1280
Número de páginas18
PublicaciónMolecular Plant
Volumen5
N.º6
DOI
EstadoPublished - 1 ene 2012

Huella dactilar

Uridine Diphosphate Glucose
Uridine Diphosphate
Arabidopsis
galactose
transporters
Uridine Diphosphate Sugars
Nucleotides
Arabidopsis thaliana
Cell Wall
sugars
glucose
nucleotides
Guanosine Diphosphate Sugars
cell walls
root hairs
Antiporters
mutants
Meristem
Esterification
Caenorhabditis elegans

ASJC Scopus subject areas

  • Molecular Biology
  • Plant Science

Citar esto

Handford, Michael ; Rodríguez-Furlán, Cecilia ; Marchant, Lorena ; Segura, Marcelo ; Gómez, Daniela ; Alvarez-Buylla, Elena ; Xiong, Guang Yan ; Pauly, Markus ; Orellana, Ariel. / Arabidopsis thaliana AtUTr7 encodes a golgi-localized UDP-glucose/UDP- galactose transporter that affects lateral root emergence. En: Molecular Plant. 2012 ; Vol. 5, N.º 6. pp. 1263-1280.
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title = "Arabidopsis thaliana AtUTr7 encodes a golgi-localized UDP-glucose/UDP- galactose transporter that affects lateral root emergence",
abstract = "Nucleotide sugar transporters (NSTs) are antiporters comprising a gene family that plays a fundamental role in the biosynthesis of complex cell wall polysaccharides and glycoproteins in plants. However, due to the limited number of related mutants that have observable phenotypes, the biological function(s) of most NSTs in cell wall biosynthesis and assembly have remained elusive. Here, we report the characterization of AtUTr7 from Arabidopsis (Arabidopsis thaliana (L.) Heynh.), which is homologous to multi-specific UDP-sugar transporters from Drosophila melanogaster, humans, and Caenorhabditis elegans. We show that AtUTr7 possesses the common structural characteristics conserved among NSTs. Using a green fluorescent protein (GFP) tagged version, we demonstrate that AtUTr7 is localized in the Golgi apparatus. We also show that AtUTr7 is widely expressed, especially in the roots and in specific floral organs. Additionally, the results of an in vitro nucleotide sugar transport assay carried out with a tobacco and a yeast expression system suggest that AtUTr7 is capable of transferring UDP-Gal and UDP-Glc, but not a range of other UDP- and GDP-sugars, into the Golgi lumen. Mutants lacking expression of AtUTr7 exhibited an early proliferation of lateral roots as well as distorted root hairs when cultivated at high sucrose concentrations. Furthermore, the distribution of homogalacturonan with a low degree of methyl esterification differed in lateral root tips of the mutant compared to wild-type plants, although additional analytical procedures revealed no further differences in the composition of the root cell walls. This evidence suggests that the transport of UDP-Gal and UDP-Glc into the Golgi under conditions of high root biomass production plays a role in lateral root and root hair development.",
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Handford, M, Rodríguez-Furlán, C, Marchant, L, Segura, M, Gómez, D, Alvarez-Buylla, E, Xiong, GY, Pauly, M & Orellana, A 2012, 'Arabidopsis thaliana AtUTr7 encodes a golgi-localized UDP-glucose/UDP- galactose transporter that affects lateral root emergence', Molecular Plant, vol. 5, n.º 6, pp. 1263-1280. https://doi.org/10.1093/mp/sss074

Arabidopsis thaliana AtUTr7 encodes a golgi-localized UDP-glucose/UDP- galactose transporter that affects lateral root emergence. / Handford, Michael; Rodríguez-Furlán, Cecilia; Marchant, Lorena; Segura, Marcelo; Gómez, Daniela; Alvarez-Buylla, Elena; Xiong, Guang Yan; Pauly, Markus; Orellana, Ariel.

En: Molecular Plant, Vol. 5, N.º 6, 01.01.2012, p. 1263-1280.

Resultado de la investigación: Article

TY - JOUR

T1 - Arabidopsis thaliana AtUTr7 encodes a golgi-localized UDP-glucose/UDP- galactose transporter that affects lateral root emergence

AU - Handford, Michael

AU - Rodríguez-Furlán, Cecilia

AU - Marchant, Lorena

AU - Segura, Marcelo

AU - Gómez, Daniela

AU - Alvarez-Buylla, Elena

AU - Xiong, Guang Yan

AU - Pauly, Markus

AU - Orellana, Ariel

PY - 2012/1/1

Y1 - 2012/1/1

N2 - Nucleotide sugar transporters (NSTs) are antiporters comprising a gene family that plays a fundamental role in the biosynthesis of complex cell wall polysaccharides and glycoproteins in plants. However, due to the limited number of related mutants that have observable phenotypes, the biological function(s) of most NSTs in cell wall biosynthesis and assembly have remained elusive. Here, we report the characterization of AtUTr7 from Arabidopsis (Arabidopsis thaliana (L.) Heynh.), which is homologous to multi-specific UDP-sugar transporters from Drosophila melanogaster, humans, and Caenorhabditis elegans. We show that AtUTr7 possesses the common structural characteristics conserved among NSTs. Using a green fluorescent protein (GFP) tagged version, we demonstrate that AtUTr7 is localized in the Golgi apparatus. We also show that AtUTr7 is widely expressed, especially in the roots and in specific floral organs. Additionally, the results of an in vitro nucleotide sugar transport assay carried out with a tobacco and a yeast expression system suggest that AtUTr7 is capable of transferring UDP-Gal and UDP-Glc, but not a range of other UDP- and GDP-sugars, into the Golgi lumen. Mutants lacking expression of AtUTr7 exhibited an early proliferation of lateral roots as well as distorted root hairs when cultivated at high sucrose concentrations. Furthermore, the distribution of homogalacturonan with a low degree of methyl esterification differed in lateral root tips of the mutant compared to wild-type plants, although additional analytical procedures revealed no further differences in the composition of the root cell walls. This evidence suggests that the transport of UDP-Gal and UDP-Glc into the Golgi under conditions of high root biomass production plays a role in lateral root and root hair development.

AB - Nucleotide sugar transporters (NSTs) are antiporters comprising a gene family that plays a fundamental role in the biosynthesis of complex cell wall polysaccharides and glycoproteins in plants. However, due to the limited number of related mutants that have observable phenotypes, the biological function(s) of most NSTs in cell wall biosynthesis and assembly have remained elusive. Here, we report the characterization of AtUTr7 from Arabidopsis (Arabidopsis thaliana (L.) Heynh.), which is homologous to multi-specific UDP-sugar transporters from Drosophila melanogaster, humans, and Caenorhabditis elegans. We show that AtUTr7 possesses the common structural characteristics conserved among NSTs. Using a green fluorescent protein (GFP) tagged version, we demonstrate that AtUTr7 is localized in the Golgi apparatus. We also show that AtUTr7 is widely expressed, especially in the roots and in specific floral organs. Additionally, the results of an in vitro nucleotide sugar transport assay carried out with a tobacco and a yeast expression system suggest that AtUTr7 is capable of transferring UDP-Gal and UDP-Glc, but not a range of other UDP- and GDP-sugars, into the Golgi lumen. Mutants lacking expression of AtUTr7 exhibited an early proliferation of lateral roots as well as distorted root hairs when cultivated at high sucrose concentrations. Furthermore, the distribution of homogalacturonan with a low degree of methyl esterification differed in lateral root tips of the mutant compared to wild-type plants, although additional analytical procedures revealed no further differences in the composition of the root cell walls. This evidence suggests that the transport of UDP-Gal and UDP-Glc into the Golgi under conditions of high root biomass production plays a role in lateral root and root hair development.

KW - cell wall

KW - Golgi apparatus

KW - nucleotide sugar transporters

KW - nucleotide sugars

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Handford M, Rodríguez-Furlán C, Marchant L, Segura M, Gómez D, Alvarez-Buylla E y otros. Arabidopsis thaliana AtUTr7 encodes a golgi-localized UDP-glucose/UDP- galactose transporter that affects lateral root emergence. Molecular Plant. 2012 ene 1;5(6):1263-1280. https://doi.org/10.1093/mp/sss074