Arabidopsis heterotrimeric G-protein regulates cell wall defense and resistance to necrotrophic fungi

Magdalena Delgado-Cerezo, Clara Sánchez-Rodríguez, Viviana Escudero, Eva Miedes, Paula Virginia Fernández, Lucía Jordá, Camilo Hernández-Blanco, Andrea Sánchez-Vallet, Pawel Bednarek, Paul Schulze-Lefert, Shauna Somerville, José Manuel Estevez, Staffan Persson, Antonio Molina

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

85 Citas (Scopus)

Resumen

The Arabidopsis heterotrimeric G-protein controls defense responses to necrotrophic and vascular fungi. The agb1 mutant impaired in the Gβ subunit displays enhanced susceptibility to these pathogens. Gβ/AGB1 forms an obligate dimer with either one of the Arabidopsis Gγ subunits (γ1/AGG1 and γ2/AGG2). Accordingly, we now demonstrate that the agg1 agg2 double mutant is as susceptible as agb1 plants to the necrotrophic fungus Plectosphaerella cucumerina. To elucidate the molecular basis of heterotrimeric G-protein-mediated resistance, we performed a comparative transcriptomic analysis of agb1-1 mutant and wild-type plants upon inoculation with P. cucumerina. This analysis, together with metabolomic studies, demonstrated that G-protein-mediated resistance was independent of defensive pathways required for resistance to necrotrophic fungi, such as the salicylic acid, jasmonic acid, ethylene, abscisic acid, and tryptophan-derived metabolites signaling, as these pathways were not impaired in agb1 and agg1 agg2 mutants. Notably, many mis-regulated genes in agb1 plants were related with cell wall functions, which was also the case in agg1 agg2 mutant. Biochemical analyses and Fourier Transform InfraRed (FTIR) spectroscopy of cell walls from G-protein mutants revealed that the xylose content was lower in agb1 and agg1 agg2 mutants than in wild-type plants, and that mutant walls had similar FTIR spectratypes, which differed from that of wild-type plants. The data presented here suggest a canonical functionality of the Gβ and Gγ1/γ2 subunits in the control of Arabidopsis immune responses and the regulation of cell wall composition.

Idioma originalInglés
Páginas (desde-hasta)98-114
Número de páginas17
PublicaciónMolecular Plant
Volumen5
N.º1
DOI
EstadoPublicada - ene 2012
Publicado de forma externa

Áreas temáticas de ASJC Scopus

  • Biología molecular
  • Botánica

Huella

Profundice en los temas de investigación de 'Arabidopsis heterotrimeric G-protein regulates cell wall defense and resistance to necrotrophic fungi'. En conjunto forman una huella única.

Citar esto