Apoptosis related genes expressed in cultured Fallopian tube epithelial cells infected in vitro with Neisseria gonorrhoeae

Paz A. Reyes, Macarena F. Vargas, Katherine P. García, Paulina S. Rubilar, Patricia A. Navarrete, Pamela M. Fuentes, Miguel A. Ríos, Pedro A. Orihuela, Renato H. Vargas, Víctor H. Rubio, John Heckels, Myron Christodoulides, Hugo Cárdenas, Luis A. Velásquez

Resultado de la investigación: Article

2 Citas (Scopus)

Resumen

Background: Infection of the Fallopian tubes (FT) by Neisseria gonorrhoeae (Ngo) can lead to acute salpingitis, an inflammatory condition resulting in damage primarily to the ciliated cells, with loss of ciliary activity and sloughing of the cells from the epithelium. Recently, we have shown that Ngo infection induced apoptosis in FT epithelium cells by a TNF-α dependent mechanism that could contribute to the cell and tissue damage observed in gonococcal salpingitis. Aim: To investigate the apoptosis-related genes expressed during apoptosis induction in cultured FT epithelial cells infected in vitro by Ngo. Materials and Methods: In the current study, we used cDNA macroarrays and real time PCR to identify and determine the expression levels of apoptosis related genes during the in vitro gonococci infection of FT epithelial cells. Results: Significant apoptosis was induced following infection with Ngo. Macroarray analysis identified the expression of multiple genes of the TNF receptor family (TNFRSF1B, -4, -6, -10A, -10B and -10D) and the Bcl-2 family (BAK1, BAX, BLK, HRK and MCL-1) without differences between controls and infected cells. This lack of difference was confirmed by RT-PCR of BAX, Bcl-2, TNFRS1A (TNFR-I) and TNFRSF1B (TNFR-II). Conclusion: Several genes related to apoptosis are expressed in primary cultures of epithelial cells of the human Fallopian tube. Infection with Ngo induces apoptosis without changes in the pattern of gene expression of several apoptosis-related genes. Results strongly suggest that Ngo regulates apoptosis in the FT by post-transcriptional mechanisms that need to be further addressed.

Idioma originalEnglish
Páginas (desde-hasta)319-327
Número de páginas9
PublicaciónBiological Research
Volumen40
N.º3
DOI
EstadoPublished - 1 ene 2007

Huella dactilar

Neisseria gonorrhoeae
fallopian tubes
Fallopian Tubes
epithelial cells
apoptosis
Genes
Epithelial Cells
Apoptosis
genes
salpingitis
Salpingitis
Infection
infection
cells
epithelium
Epithelium
Neisseria
Receptors, Tumor Necrosis Factor, Type I
Gene Expression
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Citar esto

Reyes, P. A., Vargas, M. F., García, K. P., Rubilar, P. S., Navarrete, P. A., Fuentes, P. M., ... Velásquez, L. A. (2007). Apoptosis related genes expressed in cultured Fallopian tube epithelial cells infected in vitro with Neisseria gonorrhoeae. Biological Research, 40(3), 319-327. https://doi.org/10.4067/S0716-97602007000400006
Reyes, Paz A. ; Vargas, Macarena F. ; García, Katherine P. ; Rubilar, Paulina S. ; Navarrete, Patricia A. ; Fuentes, Pamela M. ; Ríos, Miguel A. ; Orihuela, Pedro A. ; Vargas, Renato H. ; Rubio, Víctor H. ; Heckels, John ; Christodoulides, Myron ; Cárdenas, Hugo ; Velásquez, Luis A. / Apoptosis related genes expressed in cultured Fallopian tube epithelial cells infected in vitro with Neisseria gonorrhoeae. En: Biological Research. 2007 ; Vol. 40, N.º 3. pp. 319-327.
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title = "Apoptosis related genes expressed in cultured Fallopian tube epithelial cells infected in vitro with Neisseria gonorrhoeae",
abstract = "Background: Infection of the Fallopian tubes (FT) by Neisseria gonorrhoeae (Ngo) can lead to acute salpingitis, an inflammatory condition resulting in damage primarily to the ciliated cells, with loss of ciliary activity and sloughing of the cells from the epithelium. Recently, we have shown that Ngo infection induced apoptosis in FT epithelium cells by a TNF-α dependent mechanism that could contribute to the cell and tissue damage observed in gonococcal salpingitis. Aim: To investigate the apoptosis-related genes expressed during apoptosis induction in cultured FT epithelial cells infected in vitro by Ngo. Materials and Methods: In the current study, we used cDNA macroarrays and real time PCR to identify and determine the expression levels of apoptosis related genes during the in vitro gonococci infection of FT epithelial cells. Results: Significant apoptosis was induced following infection with Ngo. Macroarray analysis identified the expression of multiple genes of the TNF receptor family (TNFRSF1B, -4, -6, -10A, -10B and -10D) and the Bcl-2 family (BAK1, BAX, BLK, HRK and MCL-1) without differences between controls and infected cells. This lack of difference was confirmed by RT-PCR of BAX, Bcl-2, TNFRS1A (TNFR-I) and TNFRSF1B (TNFR-II). Conclusion: Several genes related to apoptosis are expressed in primary cultures of epithelial cells of the human Fallopian tube. Infection with Ngo induces apoptosis without changes in the pattern of gene expression of several apoptosis-related genes. Results strongly suggest that Ngo regulates apoptosis in the FT by post-transcriptional mechanisms that need to be further addressed.",
keywords = "Apoptosis, Fallopian tube, Neisseria gonorrhoeae, Salpingitis, TNF-α",
author = "Reyes, {Paz A.} and Vargas, {Macarena F.} and Garc{\'i}a, {Katherine P.} and Rubilar, {Paulina S.} and Navarrete, {Patricia A.} and Fuentes, {Pamela M.} and R{\'i}os, {Miguel A.} and Orihuela, {Pedro A.} and Vargas, {Renato H.} and Rubio, {V{\'i}ctor H.} and John Heckels and Myron Christodoulides and Hugo C{\'a}rdenas and Vel{\'a}squez, {Luis A.}",
year = "2007",
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doi = "10.4067/S0716-97602007000400006",
language = "English",
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pages = "319--327",
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Reyes, PA, Vargas, MF, García, KP, Rubilar, PS, Navarrete, PA, Fuentes, PM, Ríos, MA, Orihuela, PA, Vargas, RH, Rubio, VH, Heckels, J, Christodoulides, M, Cárdenas, H & Velásquez, LA 2007, 'Apoptosis related genes expressed in cultured Fallopian tube epithelial cells infected in vitro with Neisseria gonorrhoeae', Biological Research, vol. 40, n.º 3, pp. 319-327. https://doi.org/10.4067/S0716-97602007000400006

Apoptosis related genes expressed in cultured Fallopian tube epithelial cells infected in vitro with Neisseria gonorrhoeae. / Reyes, Paz A.; Vargas, Macarena F.; García, Katherine P.; Rubilar, Paulina S.; Navarrete, Patricia A.; Fuentes, Pamela M.; Ríos, Miguel A.; Orihuela, Pedro A.; Vargas, Renato H.; Rubio, Víctor H.; Heckels, John; Christodoulides, Myron; Cárdenas, Hugo; Velásquez, Luis A.

En: Biological Research, Vol. 40, N.º 3, 01.01.2007, p. 319-327.

Resultado de la investigación: Article

TY - JOUR

T1 - Apoptosis related genes expressed in cultured Fallopian tube epithelial cells infected in vitro with Neisseria gonorrhoeae

AU - Reyes, Paz A.

AU - Vargas, Macarena F.

AU - García, Katherine P.

AU - Rubilar, Paulina S.

AU - Navarrete, Patricia A.

AU - Fuentes, Pamela M.

AU - Ríos, Miguel A.

AU - Orihuela, Pedro A.

AU - Vargas, Renato H.

AU - Rubio, Víctor H.

AU - Heckels, John

AU - Christodoulides, Myron

AU - Cárdenas, Hugo

AU - Velásquez, Luis A.

PY - 2007/1/1

Y1 - 2007/1/1

N2 - Background: Infection of the Fallopian tubes (FT) by Neisseria gonorrhoeae (Ngo) can lead to acute salpingitis, an inflammatory condition resulting in damage primarily to the ciliated cells, with loss of ciliary activity and sloughing of the cells from the epithelium. Recently, we have shown that Ngo infection induced apoptosis in FT epithelium cells by a TNF-α dependent mechanism that could contribute to the cell and tissue damage observed in gonococcal salpingitis. Aim: To investigate the apoptosis-related genes expressed during apoptosis induction in cultured FT epithelial cells infected in vitro by Ngo. Materials and Methods: In the current study, we used cDNA macroarrays and real time PCR to identify and determine the expression levels of apoptosis related genes during the in vitro gonococci infection of FT epithelial cells. Results: Significant apoptosis was induced following infection with Ngo. Macroarray analysis identified the expression of multiple genes of the TNF receptor family (TNFRSF1B, -4, -6, -10A, -10B and -10D) and the Bcl-2 family (BAK1, BAX, BLK, HRK and MCL-1) without differences between controls and infected cells. This lack of difference was confirmed by RT-PCR of BAX, Bcl-2, TNFRS1A (TNFR-I) and TNFRSF1B (TNFR-II). Conclusion: Several genes related to apoptosis are expressed in primary cultures of epithelial cells of the human Fallopian tube. Infection with Ngo induces apoptosis without changes in the pattern of gene expression of several apoptosis-related genes. Results strongly suggest that Ngo regulates apoptosis in the FT by post-transcriptional mechanisms that need to be further addressed.

AB - Background: Infection of the Fallopian tubes (FT) by Neisseria gonorrhoeae (Ngo) can lead to acute salpingitis, an inflammatory condition resulting in damage primarily to the ciliated cells, with loss of ciliary activity and sloughing of the cells from the epithelium. Recently, we have shown that Ngo infection induced apoptosis in FT epithelium cells by a TNF-α dependent mechanism that could contribute to the cell and tissue damage observed in gonococcal salpingitis. Aim: To investigate the apoptosis-related genes expressed during apoptosis induction in cultured FT epithelial cells infected in vitro by Ngo. Materials and Methods: In the current study, we used cDNA macroarrays and real time PCR to identify and determine the expression levels of apoptosis related genes during the in vitro gonococci infection of FT epithelial cells. Results: Significant apoptosis was induced following infection with Ngo. Macroarray analysis identified the expression of multiple genes of the TNF receptor family (TNFRSF1B, -4, -6, -10A, -10B and -10D) and the Bcl-2 family (BAK1, BAX, BLK, HRK and MCL-1) without differences between controls and infected cells. This lack of difference was confirmed by RT-PCR of BAX, Bcl-2, TNFRS1A (TNFR-I) and TNFRSF1B (TNFR-II). Conclusion: Several genes related to apoptosis are expressed in primary cultures of epithelial cells of the human Fallopian tube. Infection with Ngo induces apoptosis without changes in the pattern of gene expression of several apoptosis-related genes. Results strongly suggest that Ngo regulates apoptosis in the FT by post-transcriptional mechanisms that need to be further addressed.

KW - Apoptosis

KW - Fallopian tube

KW - Neisseria gonorrhoeae

KW - Salpingitis

KW - TNF-α

UR - http://www.scopus.com/inward/record.url?scp=41849127454&partnerID=8YFLogxK

U2 - 10.4067/S0716-97602007000400006

DO - 10.4067/S0716-97602007000400006

M3 - Article

C2 - 18449459

AN - SCOPUS:41849127454

VL - 40

SP - 319

EP - 327

JO - Biological Research

JF - Biological Research

SN - 0716-9760

IS - 3

ER -