Antifungal effect of polygodial on Botrytis cinerea, a fungal pathogen affecting table grapes

Héctor Carrasco, Christian Robles-Kelly, Julia Rubio, Andrés F. Olea, Rolando Martínez, Evelyn Silva-Moreno

Resultado de la investigación: Article

2 Citas (Scopus)

Resumen

The antifungal activity of polygodial, a secondary metabolite extracted from Canelo, on mycelial growth of different Botrytis cinerea isolates has been evaluated. The results show that polygodial affects growth of normal and resistant isolates of B. cinerea with EC50 values ranging between 117 and 175 ppm. In addition, polygodial markedly decreases the germination of B. cinerea, i.e., after six hours of incubation the percentage of germination decreases from 92% (control) to 25% and 5% in the presence of 20 ppm and 80 ppm of polygodial, respectively. Morphological studies indicate that conidia treated with polygodial are smaller, with irregular membrane border, and a lot of cell debris, as compared to conidia in the control. The existence of polygodial-induced membrane damage was confirmed by SYTOX® Green uptake assay. Gene expression studies confirm that the effect of polygodial on B. cinerea is mainly attributed to inhibition of germination and appears at early stages of B. cinerea development. On the other hand, drimenol, a drimane with chemical structure quite similar to polygodial, inhibits the mycelial growth efficiently. Thus, both compounds inhibit mycelial growth by different mechanisms. The different antifungal activities of these compounds are discussed in terms of the electronic density on the double bond.

Idioma originalEnglish
Número de artículo2251
PublicaciónInternational Journal of Molecular Sciences
Volumen18
N.º11
DOI
EstadoPublished - 1 nov 2017

Huella dactilar

Botrytis
pathogens
Vitis
Pathogens
germination
Germination
membranes
Membranes
Fungal Spores
metabolites
gene expression
Growth
Metabolites
borders
debris
Debris
Gene expression
Assays
damage
polygodial

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Citar esto

Carrasco, Héctor ; Robles-Kelly, Christian ; Rubio, Julia ; Olea, Andrés F. ; Martínez, Rolando ; Silva-Moreno, Evelyn. / Antifungal effect of polygodial on Botrytis cinerea, a fungal pathogen affecting table grapes. En: International Journal of Molecular Sciences. 2017 ; Vol. 18, N.º 11.
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abstract = "The antifungal activity of polygodial, a secondary metabolite extracted from Canelo, on mycelial growth of different Botrytis cinerea isolates has been evaluated. The results show that polygodial affects growth of normal and resistant isolates of B. cinerea with EC50 values ranging between 117 and 175 ppm. In addition, polygodial markedly decreases the germination of B. cinerea, i.e., after six hours of incubation the percentage of germination decreases from 92{\%} (control) to 25{\%} and 5{\%} in the presence of 20 ppm and 80 ppm of polygodial, respectively. Morphological studies indicate that conidia treated with polygodial are smaller, with irregular membrane border, and a lot of cell debris, as compared to conidia in the control. The existence of polygodial-induced membrane damage was confirmed by SYTOX{\circledR} Green uptake assay. Gene expression studies confirm that the effect of polygodial on B. cinerea is mainly attributed to inhibition of germination and appears at early stages of B. cinerea development. On the other hand, drimenol, a drimane with chemical structure quite similar to polygodial, inhibits the mycelial growth efficiently. Thus, both compounds inhibit mycelial growth by different mechanisms. The different antifungal activities of these compounds are discussed in terms of the electronic density on the double bond.",
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Antifungal effect of polygodial on Botrytis cinerea, a fungal pathogen affecting table grapes. / Carrasco, Héctor; Robles-Kelly, Christian; Rubio, Julia; Olea, Andrés F.; Martínez, Rolando; Silva-Moreno, Evelyn.

En: International Journal of Molecular Sciences, Vol. 18, N.º 11, 2251, 01.11.2017.

Resultado de la investigación: Article

TY - JOUR

T1 - Antifungal effect of polygodial on Botrytis cinerea, a fungal pathogen affecting table grapes

AU - Carrasco, Héctor

AU - Robles-Kelly, Christian

AU - Rubio, Julia

AU - Olea, Andrés F.

AU - Martínez, Rolando

AU - Silva-Moreno, Evelyn

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N2 - The antifungal activity of polygodial, a secondary metabolite extracted from Canelo, on mycelial growth of different Botrytis cinerea isolates has been evaluated. The results show that polygodial affects growth of normal and resistant isolates of B. cinerea with EC50 values ranging between 117 and 175 ppm. In addition, polygodial markedly decreases the germination of B. cinerea, i.e., after six hours of incubation the percentage of germination decreases from 92% (control) to 25% and 5% in the presence of 20 ppm and 80 ppm of polygodial, respectively. Morphological studies indicate that conidia treated with polygodial are smaller, with irregular membrane border, and a lot of cell debris, as compared to conidia in the control. The existence of polygodial-induced membrane damage was confirmed by SYTOX® Green uptake assay. Gene expression studies confirm that the effect of polygodial on B. cinerea is mainly attributed to inhibition of germination and appears at early stages of B. cinerea development. On the other hand, drimenol, a drimane with chemical structure quite similar to polygodial, inhibits the mycelial growth efficiently. Thus, both compounds inhibit mycelial growth by different mechanisms. The different antifungal activities of these compounds are discussed in terms of the electronic density on the double bond.

AB - The antifungal activity of polygodial, a secondary metabolite extracted from Canelo, on mycelial growth of different Botrytis cinerea isolates has been evaluated. The results show that polygodial affects growth of normal and resistant isolates of B. cinerea with EC50 values ranging between 117 and 175 ppm. In addition, polygodial markedly decreases the germination of B. cinerea, i.e., after six hours of incubation the percentage of germination decreases from 92% (control) to 25% and 5% in the presence of 20 ppm and 80 ppm of polygodial, respectively. Morphological studies indicate that conidia treated with polygodial are smaller, with irregular membrane border, and a lot of cell debris, as compared to conidia in the control. The existence of polygodial-induced membrane damage was confirmed by SYTOX® Green uptake assay. Gene expression studies confirm that the effect of polygodial on B. cinerea is mainly attributed to inhibition of germination and appears at early stages of B. cinerea development. On the other hand, drimenol, a drimane with chemical structure quite similar to polygodial, inhibits the mycelial growth efficiently. Thus, both compounds inhibit mycelial growth by different mechanisms. The different antifungal activities of these compounds are discussed in terms of the electronic density on the double bond.

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