Analysis of the germination of individual Clostridium perfringens spores and its heterogeneity

G. Wang, P. Zhang, D. Paredes-Sabja, C. Green, P. Setlow, M. R. Sarker, Y. Q. Li

Resultado de la investigación: Article

30 Citas (Scopus)

Resumen

Aims: To analyse the germination and its heterogeneity of individual spores of Clostridium perfringens. Methods and Results: Germination of individual wild-type Cl. perfringens spores was followed by monitoring Ca-dipicolinic acid (CaDPA) release and by differential interference contrast (DIC) microscopy. Following the addition of KCl that acts via germinant receptors (GRs), there was a long variable lag period (T lag) with slow release of c.25% of CaDPA, then rapid release of remaining CaDPA in c.2min (ΔT release) and a parallel decrease in DIC image intensity, and a final decrease of c.25% in DIC image intensity during spore cortex hydrolysis. Spores lacking the essential cortex-lytic enzyme (CLE) (sleC spores) exhibited the same features during GR-dependent germination, but with longer average T lag values, and no decrease in DIC image intensity because of cortex hydrolysis after full CaDPA release. The T lag of wild-type spores in KCl germination was increased significantly by lower germinant concentrations and suboptimal heat activation. Wild-type and sleC spores had identical average T lag and ΔT release values in dodecylamine germination that does not utilize GRs. Conclusions: Most of these results were essentially identical to those reported for the germination of individual spores of Bacillus species. However, individual sleC Cl. perfringens spores germinated inefficiently with either KCl or exogenous CaDPA, in contrast to CLE-deficient Bacillus spores, indicating that germination of these species' spores is not completely identical. Significance and Impact of the Study: This work provides information on the kinetic germination and its heterogeneity of individual spores of Cl. perfringens. 2011 The Authors.

Idioma originalEnglish
Páginas (desde-hasta)1212-1223
Número de páginas12
PublicaciónJournal of Applied Microbiology
Volumen111
N.º5
DOI
EstadoPublished - nov 2011

Huella dactilar

Clostridium perfringens
Germination
Spores
Bacillus
Hydrolysis
Interference Microscopy
Enzymes
Hot Temperature

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biotechnology
  • Medicine(all)

Citar esto

Wang, G. ; Zhang, P. ; Paredes-Sabja, D. ; Green, C. ; Setlow, P. ; Sarker, M. R. ; Li, Y. Q. / Analysis of the germination of individual Clostridium perfringens spores and its heterogeneity. En: Journal of Applied Microbiology. 2011 ; Vol. 111, N.º 5. pp. 1212-1223.
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abstract = "Aims: To analyse the germination and its heterogeneity of individual spores of Clostridium perfringens. Methods and Results: Germination of individual wild-type Cl. perfringens spores was followed by monitoring Ca-dipicolinic acid (CaDPA) release and by differential interference contrast (DIC) microscopy. Following the addition of KCl that acts via germinant receptors (GRs), there was a long variable lag period (T lag) with slow release of c.25{\%} of CaDPA, then rapid release of remaining CaDPA in c.2min (ΔT release) and a parallel decrease in DIC image intensity, and a final decrease of c.25{\%} in DIC image intensity during spore cortex hydrolysis. Spores lacking the essential cortex-lytic enzyme (CLE) (sleC spores) exhibited the same features during GR-dependent germination, but with longer average T lag values, and no decrease in DIC image intensity because of cortex hydrolysis after full CaDPA release. The T lag of wild-type spores in KCl germination was increased significantly by lower germinant concentrations and suboptimal heat activation. Wild-type and sleC spores had identical average T lag and ΔT release values in dodecylamine germination that does not utilize GRs. Conclusions: Most of these results were essentially identical to those reported for the germination of individual spores of Bacillus species. However, individual sleC Cl. perfringens spores germinated inefficiently with either KCl or exogenous CaDPA, in contrast to CLE-deficient Bacillus spores, indicating that germination of these species' spores is not completely identical. Significance and Impact of the Study: This work provides information on the kinetic germination and its heterogeneity of individual spores of Cl. perfringens. 2011 The Authors.",
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Analysis of the germination of individual Clostridium perfringens spores and its heterogeneity. / Wang, G.; Zhang, P.; Paredes-Sabja, D.; Green, C.; Setlow, P.; Sarker, M. R.; Li, Y. Q.

En: Journal of Applied Microbiology, Vol. 111, N.º 5, 11.2011, p. 1212-1223.

Resultado de la investigación: Article

TY - JOUR

T1 - Analysis of the germination of individual Clostridium perfringens spores and its heterogeneity

AU - Wang, G.

AU - Zhang, P.

AU - Paredes-Sabja, D.

AU - Green, C.

AU - Setlow, P.

AU - Sarker, M. R.

AU - Li, Y. Q.

PY - 2011/11

Y1 - 2011/11

N2 - Aims: To analyse the germination and its heterogeneity of individual spores of Clostridium perfringens. Methods and Results: Germination of individual wild-type Cl. perfringens spores was followed by monitoring Ca-dipicolinic acid (CaDPA) release and by differential interference contrast (DIC) microscopy. Following the addition of KCl that acts via germinant receptors (GRs), there was a long variable lag period (T lag) with slow release of c.25% of CaDPA, then rapid release of remaining CaDPA in c.2min (ΔT release) and a parallel decrease in DIC image intensity, and a final decrease of c.25% in DIC image intensity during spore cortex hydrolysis. Spores lacking the essential cortex-lytic enzyme (CLE) (sleC spores) exhibited the same features during GR-dependent germination, but with longer average T lag values, and no decrease in DIC image intensity because of cortex hydrolysis after full CaDPA release. The T lag of wild-type spores in KCl germination was increased significantly by lower germinant concentrations and suboptimal heat activation. Wild-type and sleC spores had identical average T lag and ΔT release values in dodecylamine germination that does not utilize GRs. Conclusions: Most of these results were essentially identical to those reported for the germination of individual spores of Bacillus species. However, individual sleC Cl. perfringens spores germinated inefficiently with either KCl or exogenous CaDPA, in contrast to CLE-deficient Bacillus spores, indicating that germination of these species' spores is not completely identical. Significance and Impact of the Study: This work provides information on the kinetic germination and its heterogeneity of individual spores of Cl. perfringens. 2011 The Authors.

AB - Aims: To analyse the germination and its heterogeneity of individual spores of Clostridium perfringens. Methods and Results: Germination of individual wild-type Cl. perfringens spores was followed by monitoring Ca-dipicolinic acid (CaDPA) release and by differential interference contrast (DIC) microscopy. Following the addition of KCl that acts via germinant receptors (GRs), there was a long variable lag period (T lag) with slow release of c.25% of CaDPA, then rapid release of remaining CaDPA in c.2min (ΔT release) and a parallel decrease in DIC image intensity, and a final decrease of c.25% in DIC image intensity during spore cortex hydrolysis. Spores lacking the essential cortex-lytic enzyme (CLE) (sleC spores) exhibited the same features during GR-dependent germination, but with longer average T lag values, and no decrease in DIC image intensity because of cortex hydrolysis after full CaDPA release. The T lag of wild-type spores in KCl germination was increased significantly by lower germinant concentrations and suboptimal heat activation. Wild-type and sleC spores had identical average T lag and ΔT release values in dodecylamine germination that does not utilize GRs. Conclusions: Most of these results were essentially identical to those reported for the germination of individual spores of Bacillus species. However, individual sleC Cl. perfringens spores germinated inefficiently with either KCl or exogenous CaDPA, in contrast to CLE-deficient Bacillus spores, indicating that germination of these species' spores is not completely identical. Significance and Impact of the Study: This work provides information on the kinetic germination and its heterogeneity of individual spores of Cl. perfringens. 2011 The Authors.

KW - Clostridium perfringens

KW - Dipicolinic acid

KW - Spore germination

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